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Background High-grade B-cell lymphomas (HGBLs), with c-Myc, Bcl2, and/or Bcl6 rearrangement, are aggressive neoplasms with poor clinical outcomes. Cyclin D1 is a proto-oncogene that is generally expressed by mantle cell lymphoma, its variants, and many other neoplasms. Aim The aim of this study is to investigate the expression and frequency of cyclin D1 in HGBL and its association with double expressor and triple expressor lymphomas. A few pieces of research have been reported on the expression of cyclin D1 in triple-hit lymphoma, renaming it quadruple-hit lymphoma. In the present study, we also used immunohistochemical (IHC) stains to look at the expression of cyclin D1 in double expressor and triple expressor. Methodology This is a cross-sectional descriptive study involving 60 cases, including both double and triple expressors, diagnosed by using hematoxylin and eosin (H&E) staining with subsequent IHC stains. We analyzed the expression of cyclin D1 in double expressor and triple expressor. Results The expression and frequency of cyclin D1 were interpreted in terms of positivity in double expressors and triple expressors. Cyclin D1 positivity was observed in three (5%) cases of double expressors and two (3.33%) cases of triple expressors. Overall, five (9%) cases of HGBL were positive for cyclin D1. Conclusion This study showed a very low frequency of cyclin D1 expression in double and triple expressor lymphomas. However, a few cases of HGBL showed expression of cyclin D1 and were not limited to only mantle cell lymphoma and its variants.
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Introduction Among female genital tract-related malignancies, ovarian tumors are the leading cause of mortality. The present study was conducted to identify the various histopathological spectrums of ovarian neoplasm (ON) according to the World Health Organization (WHO) classification in a single center. Material and methods This cross-sectional study was conducted over a period of one year (November 2020 to October 2021) in the Department of Histopathology, Chughtai Institute of Pathology (CIP), Lahore, Pakistan. The study included 390 cases of ovarian neoplasms (ONs). After proper fixation and thorough gross examination, sections were routinely processed and examined. The distribution of the various histomorphological spectrum of ovarian tumors was studied according to the WHO classification. Results Out of the 390 cases studied, 320 (82.05%) were benign, 11 (2.82%) borderline, 57 (14.61%) malignant, and two (0.52%) metastatic tumors. Surface epithelial tumors (SETs) (246 (63.08%)) were the most common of all, followed by germ cell tumors (GCTs) (115 (29.48%)) and sex cord-stromal tumors (SSTs) (27 (6.92%)). The largest percentage (36.15%) of ONs was seen in 21-30 years of age group. Conclusion The present study shows various histopathological features of ONs. Benign tumors are more common than malignant tumors. Among the benign tumors, serous tumors were most common of all. Thus, an accurate histological diagnosis is important to initiate a proper management plan.
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Background The term triple-negative breast cancer (TNBC) refers to a particular class of aggressive, poorly differentiated neoplasms that show the absence of estrogen (ER), progesterone (PR), and human epidermal growth factor receptor 2 (HER2) antibodies. SOX10 (SRY-related HMG-box 10) is a nuclear transcription factor that is commonly used to identify cancers of neural origin, but it has recently been linked to TNBC. The purpose of this study is to determine SOX10 expression in TNBC, its association with tumor grade for molecular categorization, and to determine the diagnostic significance of SOX10 in TNBC at the metastatic site in the case of an unknown primary. Methodology SOX10 was used to stain a tissue microarray of 100 patients. According to the tumor grade, SOX10 staining was classified as negative (<1%), patchy (1-10%), focal (10-70%), and diffuse (70-100%). SPSS version 22 (IBM Corp., Armonk, NY, USA) software was used to conduct the statistical analysis. Results The expression of SOX10 regarding positivity and intensity was higher in high-grade tumors than in intermediate-grade tumors (p = 0.001 and p = 0.007, respectively). Conclusions SOX10 is a reliable novel marker for the diagnosis of TNBC and has diagnostic utility in the unknown primary at the metastatic site.
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OBJECTIVES: In this report, we share our experience about the common types of childhood hepatic tumours during 10 years (2001-2010) and compare them with other studies. METHODS: During 10 years (2001-2010), all the hepatic tumours of childhood received at Pathology Department of the Children Hospital and Institute of Child Health, Lahore Pakistan are recorded. This includes both resected specimens and biopsies. All the slides were reviewed and the pathologic diagnosis was confirmed. RESULTS: We diagnosed 48 liver tumour cases in children (below 18 years of age). Among these tumours, 39 (81.25%) were malignant. Male to female ratio was 2:1. Hepatoblastoma was the most common liver tumour in this age group accounting for 69.23% of all malignant tumours (27 cases). The second most common primary tumour was hepatocellular carcinoma diagnosed in six patients (15.38%). Other malignant tumours were undifferentiated embryonal sarcoma and biliary rhabdomyosarcoma. Benign tumours included mesenchymal hamartoma, infantile haemangioendotheloima, hemangioma and benign cyst. There were also three metastatic tumours during these 10 years. In one case there was tumour necrosis only and as such no definitive diagnosis was rendered. CONCLUSION: The spectrum of hepatic tumours in children is different from that found in the older age group and most of them are malignant.
