ABSTRACT
The influence of delta sleep-inducing peptide (DSIP, 60 and 120 nmole/kg, intraperitoneally) on the content of substance P (SP) in the hypothalamus of rats was studied in male rats of the August line. It was demonstrated that the administration of DSIP significantly increases the average content of SP in the hypothalamus, as well as its content in animals resistant to and predisposed to emotional stress. A daily one-time administration of DSIP before placing the rats in conditions of stress increases the content of SP in the hypothalamus which was decreased during emotional stress. The preliminary one-time administration of DSIP to animals subjected to a stressor influence also increases the SP content in the hypothalamus. It was established that a one-time administration of DSIP in a dose of 60 nmole/kg sharply decreases the classical manifestations of stress such as the hypertrophy of the adrenals and involution of the thymus.
Subject(s)
Delta Sleep-Inducing Peptide/pharmacology , Emotions/drug effects , Hypothalamus/metabolism , Stress, Psychological/prevention & control , Substance P/metabolism , Adrenal Glands/drug effects , Adrenal Glands/metabolism , Animals , Hypothalamus/drug effects , Male , Organ Size/drug effects , Rats , Stress, Psychological/psychology , Thymus Gland/drug effects , Thymus Gland/metabolismABSTRACT
The influence of the delta-sleep inducing peptide (DSIP, 60 and 120 nmol/kg, intraperitoneally) on the content of substance P (SP) in rats hypothalamus was studied on males of August line. DSIP administration significantly increased the mean SP content in the hypothalamus and also its content in animals, stable and predisposed to emotional stress. Daily DSIP administration before putting the rats in conditions of stress increased the SP content in the hypothalamus decreased at the emotional stress. Preliminary single DSIP administration to the animals subjected to stress also increased the SP content. Single DSIP administration in a dose of 60 nmol/kg sharply reduced classical stress manifestations, such as hypertrophy of adrenals and thymus involution.
Subject(s)
Delta Sleep-Inducing Peptide/pharmacology , Hypothalamus/drug effects , Stress, Psychological/physiopathology , Substance P/drug effects , Animals , Delta Sleep-Inducing Peptide/therapeutic use , Dose-Response Relationship, Drug , Drug Evaluation, Preclinical , Hypothalamus/chemistry , Hypothalamus/physiology , Male , Rats , Rats, Inbred Strains , Stress, Psychological/prevention & control , Substance P/analysis , Substance P/physiologyABSTRACT
A study was made of the content of substance P in the blood and hypothalamus of Wistar rat brain in acute and chronic emotional stress and after intraperitoneal injection of substance P in a dose of 250 mg/kg. A possibility was demonstrated of inducing long-term changes in the content of substance P in the hypothalamus after a single injection. Exposure to a single 24-hour stress was followed by an increase in the substance P content in the hypothalamus.