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1.
J Biol Chem ; 300(5): 107283, 2024 May.
Article in English | MEDLINE | ID: mdl-38608728

ABSTRACT

Over the past 3 decades, a diverse collection of small protein domains have been used as scaffolds to generate general purpose protein-binding reagents using a variety of protein display and enrichment technologies. To expand the repertoire of scaffolds and protein surfaces that might serve this purpose, we have explored the utility of (i) a pair of anti-parallel alpha-helices in a small highly disulfide-bonded 4-helix bundle, the CC4 domain from reversion-inducing Cysteine-rich Protein with Kazal Motifs and (ii) a concave beta-sheet surface and two adjacent loops in the human FN3 domain, the scaffold for the widely used monobody platform. Using M13 phage display and next generation sequencing, we observe that, in both systems, libraries of ∼30 million variants contain binding proteins with affinities in the low µM range for baits corresponding to the extracellular domains of multiple mammalian proteins. CC4- and FN3-based binding proteins were fused to the N- and/or C-termini of Fc domains and used for immunostaining of transfected cells. Additionally, FN3-based binding proteins were inserted into VP1 of AAV to direct AAV infection to cells expressing a defined surface receptor. Finally, FN3-based binding proteins were inserted into the Pvc13 tail fiber protein of an extracellular contractile injection system particle to direct protein cargo delivery to cells expressing a defined surface receptor. These experiments support the utility of CC4 helices B and C and of FN3 beta-strands C, D, and F together with adjacent loops CD and FG as surfaces for engineering general purpose protein-binding reagents.


Subject(s)
Peptide Library , Protein Conformation, alpha-Helical , Protein Conformation, beta-Strand , Animals , Humans , Bacteriophage M13 , Cell Surface Display Techniques , HEK293 Cells , Protein Binding
2.
Proc Natl Acad Sci U S A ; 120(42): e2311983120, 2023 10 17.
Article in English | MEDLINE | ID: mdl-37812717

ABSTRACT

The lacrimal gland is of central interest in ophthalmology both as the source of the aqueous component of tear fluid and as the site of autoimmune pathology in the context of Sjogren's syndrome (SjS). To provide a foundational description of mouse lacrimal gland cell types and their patterns of gene expression, we have analyzed single-cell transcriptomes from wild-type (Balb/c) mice and from two genetically based SjS models, MRL/lpr and NOD (nonobese diabetic).H2b, and defined the localization of multiple cell-type-specific protein and mRNA markers. This analysis has uncovered a previously undescribed cell type, Car6+ cells, which are located at the junction of the acini and the connecting ducts. More than a dozen secreted polypeptides that are likely to be components of tear fluid are expressed by acinar cells and show pronounced sex differences in expression. Additional examples of gene expression heterogeneity within a single cell type were identified, including a gradient of Claudin4 along the length of the ductal system and cell-to-cell heterogeneity in transcription factor expression within acinar and myoepithelial cells. The patterns of expression of channels, transporters, and pumps in acinar, Car6+, and ductal cells make strong predictions regarding the mechanisms of water and electrolyte secretion. In MRL/lpr and NOD.H2b lacrimal glands, distinctive changes in parenchymal gene expression and in immune cell subsets reveal widespread interferon responses, a T cell-dominated infiltrate in the MRL/lpr model, and a mixed B cell and T cell infiltrate in the NOD.H2b model.


Subject(s)
Lacrimal Apparatus , Sjogren's Syndrome , Female , Mice , Male , Animals , Sjogren's Syndrome/metabolism , Lacrimal Apparatus/metabolism , Mice, Inbred MRL lpr , Mice, Inbred NOD , Mice, Inbred BALB C , Disease Models, Animal
3.
Elife ; 122023 Jun 15.
Article in English | MEDLINE | ID: mdl-37318981

ABSTRACT

Bacterial meningitis is a major cause of morbidity and mortality, especially among infants and the elderly. Here, we study mice to assess the response of each of the major meningeal cell types to early postnatal E. coli infection using single nucleus RNA sequencing (snRNAseq), immunostaining, and genetic and pharamacologic perturbations of immune cells and immune signaling. Flatmounts of the dissected leptomeninges and dura were used to facilitiate high-quality confocal imaging and quantification of cell abundances and morphologies. Upon infection, the major meningeal cell types - including endothelial cells (ECs), macrophages, and fibroblasts - exhibit distinctive changes in their transcriptomes. Additionally, ECs in the leptomeninges redistribute CLDN5 and PECAM1, and leptomeningeal capillaries exhibit foci with reduced blood-brain barrier integrity. The vascular response to infection appears to be largely driven by TLR4 signaling, as determined by the nearly identical responses induced by infection and LPS administration and by the blunted response to infection in Tlr4-/- mice. Interestingly, knocking out Ccr2, encoding a major chemoattractant for monocytes, or acute depletion of leptomeningeal macrophages, following intracebroventricular injection of liposomal clodronate, had little or no effect on the response of leptomeningeal ECs to E. coli infection. Taken together, these data imply that EC responses to infection are largely driven by the intrinsic EC response to LPS.


Subject(s)
Endothelial Cells , Meningitis, Bacterial , Mice , Animals , Endothelial Cells/metabolism , Lipopolysaccharides/metabolism , Escherichia coli/genetics , Toll-Like Receptor 4/genetics , Toll-Like Receptor 4/metabolism
4.
Annu Rev Neurosci ; 45: 87-108, 2022 07 08.
Article in English | MEDLINE | ID: mdl-35803586

ABSTRACT

During development, the central nervous system (CNS) vasculature grows to precisely meet the metabolic demands of neurons and glia. In addition, the vast majority of the CNS vasculature acquires a unique set of molecular and cellular properties-collectively referred to as the blood-brain barrier-that minimize passive diffusion of molecules between the blood and the CNS parenchyma. Both of these processes are controlled by signals emanating from neurons and glia. In this review, we describe the nature and mechanisms-of-action of these signals, with an emphasis on vascular endothelial growth factor (VEGF) and beta-catenin (canonical Wnt) signaling, the two best-understood systems that regulate CNS vascular development. We highlight foundational discoveries, interactions between different signaling systems, the integration of genetic and cell biological studies, advances that are of clinical relevance, and questions for future research.


Subject(s)
Vascular Endothelial Growth Factor A , Wnt Signaling Pathway , Blood-Brain Barrier/metabolism , Central Nervous System , Vascular Endothelial Growth Factor A/metabolism , Wnt Signaling Pathway/physiology
5.
Elife ; 102021 11 16.
Article in English | MEDLINE | ID: mdl-34783308

ABSTRACT

The iris controls the level of retinal illumination by controlling pupil diameter. It is a site of diverse ophthalmologic diseases and it is a potential source of cells for ocular auto-transplantation. The present study provides foundational data on the mouse iris based on single nucleus RNA sequencing. More specifically, this work has (1) defined all of the major cell types in the mouse iris and ciliary body, (2) led to the discovery of two types of iris stromal cells and two types of iris sphincter cells, (3) revealed the differences in cell type-specific transcriptomes in the resting vs. dilated states, and (4) identified and validated antibody and in situ hybridization probes that can be used to visualize the major iris cell types. By immunostaining for specific iris cell types, we have observed and quantified distortions in nuclear morphology associated with iris dilation and clarified the neural crest contribution to the iris by showing that Wnt1-Cre-expressing progenitors contribute to nearly all iris cell types, whereas Sox10-Cre-expressing progenitors contribute only to stromal cells. This work should be useful as a point of reference for investigations of iris development, disease, and pharmacology, for the isolation and propagation of defined iris cell types, and for iris cell engineering and transplantation.


Subject(s)
Iris/cytology , Iris/metabolism , Transcriptome , Animals , Ciliary Body/metabolism , Female , Mice , Mice, Transgenic , Neural Crest , Pupil/physiology , Sequence Analysis, RNA
6.
Elife ; 92020 10 21.
Article in English | MEDLINE | ID: mdl-33084572

ABSTRACT

In the hippocampus, a widely accepted model posits that the dentate gyrus improves learning and memory by enhancing discrimination between inputs. To test this model, we studied conditional knockout mice in which the vast majority of dentate granule cells (DGCs) fail to develop - including nearly all DGCs in the dorsal hippocampus - secondary to eliminating Wntless (Wls) in a subset of cortical progenitors with Gfap-Cre. Other cells in the Wlsfl/-;Gfap-Cre hippocampus were minimally affected, as determined by single nucleus RNA sequencing. CA3 pyramidal cells, the targets of DGC-derived mossy fibers, exhibited normal morphologies with a small reduction in the numbers of synaptic spines. Wlsfl/-;Gfap-Cre mice have a modest performance decrement in several complex spatial tasks, including active place avoidance. They were also modestly impaired in one simpler spatial task, finding a visible platform in the Morris water maze. These experiments support a role for DGCs in enhancing spatial learning and memory.


Subject(s)
Avoidance Learning , Dentate Gyrus/abnormalities , Memory , Receptors, G-Protein-Coupled/genetics , Spatial Learning , Animals , Dentate Gyrus/growth & development , Dentate Gyrus/physiopathology , Disease Models, Animal , Female , Male , Mice , Mice, Knockout , Morris Water Maze Test , Receptors, G-Protein-Coupled/metabolism , Sequence Analysis, RNA
7.
J Clin Invest ; 129(9): 3807-3820, 2019 08 12.
Article in English | MEDLINE | ID: mdl-31403471

ABSTRACT

Vascular development in the mammalian retina is a paradigm for CNS vascular development in general, and its study is revealing fundamental mechanisms that explain the efficacy of antiangiogenic therapies in retinal vascular disease. During development of the mammalian retina, hypoxic astrocytes are hypothesized to secrete VEGF, which attracts growing endothelial cells as they migrate radially from the optic disc. However, published tests of this model using astrocyte-specific deletion of Vegf in the developing mouse retina appear to contradict this theory. Here, we report that selectively eliminating Vegf in neonatal retinal astrocytes with a Gfap-Cre line that recombines with approximately 100% efficiency had no effect on proliferation or radial migration of astrocytes, but completely blocked radial migration of endothelial cells, strongly supporting the hypoxic astrocyte model. Using additional Cre driver lines, we found evidence for essential and partially redundant actions of retina-derived (paracrine) and astrocyte-derived (autocrine) VEGF in controlling astrocyte proliferation and migration. We also extended previous studies by showing that HIF-1α in retinal neurons and HIF-2α in Müller glia play distinct roles in retinal vascular development and disease, adding to a growing body of data that point to the specialization of these 2 hypoxia-sensing transcription factors.


Subject(s)
Basic Helix-Loop-Helix Transcription Factors/metabolism , Brain/metabolism , Hypoxia-Inducible Factor 1, alpha Subunit/metabolism , Neovascularization, Physiologic , Retina/metabolism , Vascular Endothelial Growth Factor A/metabolism , Animals , Astrocytes/metabolism , Brain/blood supply , Cell Movement , Cell Proliferation , Cerebral Cortex/metabolism , Endothelial Cells/metabolism , Genotype , Green Fluorescent Proteins/metabolism , Hypoxia , Mice , Phenotype , Retinal Vessels/growth & development
8.
Proc Natl Acad Sci U S A ; 116(18): 9103-9114, 2019 04 30.
Article in English | MEDLINE | ID: mdl-30988181

ABSTRACT

The mammalian CNS is capable of tolerating chronic hypoxia, but cell type-specific responses to this stress have not been systematically characterized. In the Norrin KO (NdpKO ) mouse, a model of familial exudative vitreoretinopathy (FEVR), developmental hypovascularization of the retina produces chronic hypoxia of inner nuclear-layer (INL) neurons and Muller glia. We used single-cell RNA sequencing, untargeted metabolomics, and metabolite labeling from 13C-glucose to compare WT and NdpKO retinas. In NdpKO retinas, we observe gene expression responses consistent with hypoxia in Muller glia and retinal neurons, and we find a metabolic shift that combines reduced flux through the TCA cycle with increased synthesis of serine, glycine, and glutathione. We also used single-cell RNA sequencing to compare the responses of individual cell types in NdpKO retinas with those in the hypoxic cerebral cortex of mice that were housed for 1 week in a reduced oxygen environment (7.5% oxygen). In the hypoxic cerebral cortex, glial transcriptome responses most closely resemble the response of Muller glia in the NdpKO retina. In both retina and brain, vascular endothelial cells activate a previously dormant tip cell gene expression program, which likely underlies the adaptive neoangiogenic response to chronic hypoxia. These analyses of retina and brain transcriptomes at single-cell resolution reveal both shared and cell type-specific changes in gene expression in response to chronic hypoxia, implying both shared and distinct cell type-specific physiologic responses.


Subject(s)
Hypoxia/metabolism , Neuroglia/metabolism , Neurons/metabolism , Animals , Brain/metabolism , Disease Models, Animal , Endothelial Cells/metabolism , Familial Exudative Vitreoretinopathies/genetics , Familial Exudative Vitreoretinopathies/physiopathology , Female , Male , Mice , Mice, Inbred C57BL , Nerve Tissue Proteins/metabolism , Retina/metabolism , Retina/physiology , Retinal Neurons/metabolism , Retinal Vessels/metabolism , Sequence Analysis, RNA/methods , Single-Cell Analysis/methods
9.
Elife ; 82019 04 01.
Article in English | MEDLINE | ID: mdl-30932813

ABSTRACT

The brain, spinal cord, and retina are supplied by capillaries that do not permit free diffusion of molecules between serum and parenchyma, a property that defines the blood-brain and blood-retina barriers. Exceptions to this pattern are found in circumventricular organs (CVOs), small midline brain structures that are supplied by high permeability capillaries. In the eye and brain, high permeability capillaries are also present in the choriocapillaris, which supplies the retinal pigment epithelium and photoreceptors, and the ciliary body and choroid plexus, the sources of aqueous humor and cerebrospinal fluid, respectively. We show here that (1) endothelial cells in these high permeability vascular systems have very low beta-catenin signaling compared to barrier-competent endothelial cells, and (2) elevating beta-catenin signaling leads to a partial conversion of permeable endothelial cells to a barrier-type state. In one CVO, the area postrema, high permeability is maintained, in part, by local production of Wnt inhibitory factor-1.


Subject(s)
Capillary Permeability , Choroid/physiology , Circumventricular Organs/physiology , Gene Expression Regulation , Signal Transduction , beta Catenin/metabolism , Animals , Blood-Brain Barrier , Blood-Retinal Barrier , Endothelial Cells/physiology , Mice
10.
Elife ; 72018 10 22.
Article in English | MEDLINE | ID: mdl-30345971

ABSTRACT

Defining protein-protein interactions (PPIs) is central to the biological sciences. Here, we present a novel platform - Affinity Capture of Polyribosomes followed by RNA sequencing (ACAPseq) - for identifying PPIs. ACAPseq harnesses the power of massively parallel RNA sequencing (RNAseq) to quantify the enrichment of polyribosomes based on the affinity of their associated nascent polypeptides for an immobilized protein 'bait'. This method was developed and tested using neonatal mouse brain polyribosomes and a variety of extracellular domains as baits. Of 92 baits tested, 25 identified one or more binding partners that appear to be biologically relevant; additional candidate partners remain to be validated. ACAPseq can detect binding to targets that are present at less than 1 part in 100,000 in the starting polyribosome preparation. One of the observed PPIs was analyzed in detail, revealing the mode of homophilic binding for Protocadherin-9 (PCDH9), a non-clustered Protocadherin family member.


Subject(s)
High-Throughput Nucleotide Sequencing , Polyribosomes/genetics , Protein Interaction Mapping/methods , Protein Interaction Maps/genetics , Animals , Animals, Newborn/growth & development , Animals, Newborn/metabolism , Brain/growth & development , Brain/metabolism , Mice
11.
Elife ; 72018 09 06.
Article in English | MEDLINE | ID: mdl-30188322

ABSTRACT

Vascular endothelial cell (EC) function depends on appropriate organ-specific molecular and cellular specializations. To explore genomic mechanisms that control this specialization, we have analyzed and compared the transcriptome, accessible chromatin, and DNA methylome landscapes from mouse brain, liver, lung, and kidney ECs. Analysis of transcription factor (TF) gene expression and TF motifs at candidate cis-regulatory elements reveals both shared and organ-specific EC regulatory networks. In the embryo, only those ECs that are adjacent to or within the central nervous system (CNS) exhibit canonical Wnt signaling, which correlates precisely with blood-brain barrier (BBB) differentiation and Zic3 expression. In the early postnatal brain, single-cell RNA-seq of purified ECs reveals (1) close relationships between veins and mitotic cells and between arteries and tip cells, (2) a division of capillary ECs into vein-like and artery-like classes, and (3) new endothelial subtype markers, including new validated tip cell markers.


Subject(s)
Central Nervous System/cytology , Endothelial Cells/cytology , Endothelial Cells/metabolism , Epigenesis, Genetic , Transcription, Genetic , Amino Acid Motifs , Animals , Base Sequence , Brain/metabolism , Chromatin/metabolism , DNA Methylation/genetics , Dopa Decarboxylase/metabolism , Green Fluorescent Proteins/metabolism , Liver/metabolism , Lung/metabolism , Male , Mice, Transgenic , Multigene Family , Organ Specificity , RNA, Messenger/genetics , RNA, Messenger/metabolism , Sequence Analysis, RNA , Single-Cell Analysis , Transcription Factors/metabolism , Wnt Signaling Pathway
12.
Dev Biol ; 419(2): 298-310, 2016 11 15.
Article in English | MEDLINE | ID: mdl-27612405

ABSTRACT

The dorsal surface of the mouse tongue is covered by ~7000 papillae, asymmetric epithelial protrusions that are precisely oriented to create a stereotyped macroscopic pattern. Within the context of this large-scale pattern, neighboring papillae exhibit a high degree of local order that minimizes the differences in their orientations. We show here that the orientations of lingual papillae are under the control of the core planar cell polarity (PCP) genes Vangl1, Vangl2, and Celsr1. Using K14-Cre and Nkx2.5-Cre to induce conditional knockout of Vangl1 and/or Vangl2 in the tongue epithelium, we observe more severe disruptions to local order among papillae with inactivation of larger numbers of Vangl genes, a greater role for Vangl2 than Vangl1, and a more severe phenotype with the Vangl2 Looptail (Lp) allele than the Vangl2 null allele, consistent with a dominant negative mode of action of the Vangl2Lp allele. Interestingly, Celsr1-/- tongues show disruption of both local and global order, with many papillae in the anterior tongue showing a reversed orientation. To quantify each of these phenotypes, we have developed and applied three procedures for sampling the orientations of papillae and assessing the degree of order on different spatial scales. The experiments reported here establish the dorsal surface of the mouse tongue as a favorable system for studying PCP control of epithelial patterning.


Subject(s)
Body Patterning/physiology , Carrier Proteins/physiology , Membrane Proteins/physiology , Mice/anatomy & histology , Nerve Tissue Proteins/physiology , Receptors, G-Protein-Coupled/physiology , Tongue/anatomy & histology , Alleles , Animals , Body Patterning/genetics , Carrier Proteins/genetics , Cell Polarity/physiology , Epithelial Cells/metabolism , Gene Deletion , Gene Dosage , Gene Knockout Techniques , Genetic Association Studies , Membrane Proteins/deficiency , Membrane Proteins/genetics , Models, Biological , Nerve Tissue Proteins/deficiency , Nerve Tissue Proteins/genetics , Phenotype , Receptors, G-Protein-Coupled/deficiency , Receptors, G-Protein-Coupled/genetics , Tongue/embryology
13.
Curr Top Dev Biol ; 117: 113-39, 2016.
Article in English | MEDLINE | ID: mdl-26969975

ABSTRACT

Frizzled proteins are the principal receptors for the Wnt family of ligands. They mediate canonical Wnt signaling together with Lrp5 and Lrp6 coreceptors. In conjunction with Celsr, Vangl, and a small number of additional membrane and membrane-associated proteins, they also play a central role in tissue polarity/planar cell polarity (PCP) signaling. Targeted mutations in 9 of the 10 mammalian Frizzled genes have revealed their roles in an extraordinarily diverse set of developmental and homeostatic processes, including morphogenetic movements responsible for palate, ventricular septum, ocular furrow, and neural tube closure; survival of thalamic neurons; bone formation; central nervous system (CNS) angiogenesis and blood-brain barrier formation and maintenance; and a wide variety of processes that orient subcellular, cellular, and multicellular structures relative to the body axes. The last group likely reflects the mammalian equivalent of tissue polarity/PCP signaling, as defined in Drosophila, and it includes CNS axon guidance, hair follicle and tongue papilla orientation, and inner ear sensory hair bundle orientation. Frizzled receptors are ubiquitous among multicellular animals and, with other signaling molecules, they very likely evolved to permit the development of the complex tissue architectures that provide multicellular animals with their enormous selective advantage.


Subject(s)
Cell Polarity , Disease , Frizzled Receptors/metabolism , Hair Follicle/cytology , Neurons/cytology , Animals , Hair Follicle/metabolism , Humans , Neurons/metabolism , Signal Transduction
14.
Invest Ophthalmol Vis Sci ; 55(12): 8614-25, 2014 Nov 20.
Article in English | MEDLINE | ID: mdl-25414188

ABSTRACT

PURPOSE: To define the role of hypoxia and vascular endothelial growth factor (VEGF) in modifying the pattern, density, and permeability of the retinal vasculature in mouse models in which Norrin/Frizzled4 signaling is impaired. METHODS: Retinal vascular structure was analyzed in mice with mutation of Ndp (the gene coding for Norrin) or Frizzle4 (Fz4) with or without three additional perturbations: (1) retinal hyperoxia and reduction of VEGF, (2) reduced induction of VEGF in response to hypoxia, or (3) reduced responsiveness of vascular endothelial cells (ECs) to VEGF. These perturbations were produced, respectively, by (1) genetic ablation of rod photoreceptors in the retinal degeneration 1 (rd1) mutant background, (2) conditional deletion of the gene coding for hypoxia-inducible factor (HIF)-2alpha either in all neural retina cells or specifically in Müller glia, and (3) conditional deletion of the VEGF coreceptor neuropilin1 (NRP1) in ECs. RESULTS: All three conditions reduced vascular proliferation. Eliminating HIF2-alpha in Müller glia blocked VEGF induction in the inner nuclear layer, identifying HIF2-alpha as the transcription factor responsible for the hypoxia response in these cells. When Norrin/Frizzled4 signaling was eliminated, a secondary elevation in VEGF levels was required to compromise the barrier to transendothelial movement of high molecular weight compounds. CONCLUSIONS: In the absence of Norrin or Frizzled4, the vascular phenotype is determined by the primary defect in Norrin/Frizzled4 signaling (i.e., canonical Wnt signaling) and compensatory responses resulting from hypoxia. This work may be useful in guiding therapeutic strategies for the treatment of familial exudative vitreoretinopathy (FEVR).


Subject(s)
Eye Proteins/physiology , Frizzled Receptors/physiology , Hypoxia/physiopathology , Nerve Tissue Proteins/metabolism , Retinal Vessels/physiopathology , Signal Transduction/physiology , Animals , Basic Helix-Loop-Helix Transcription Factors/physiology , Disease Models, Animal , Endothelium, Vascular/physiopathology , Ependymoglial Cells/metabolism , Eye Proteins/metabolism , Mice , Mice, Transgenic , Neuropilin-1/physiology , Phenotype , Retinal Vessels/growth & development , Vascular Endothelial Growth Factor A/metabolism
15.
J Clin Invest ; 124(9): 3825-46, 2014 Sep.
Article in English | MEDLINE | ID: mdl-25083995

ABSTRACT

Canonical WNT signaling is required for proper vascularization of the CNS during embryonic development. Here, we used mice with targeted mutations in genes encoding canonical WNT pathway members to evaluate the exact contribution of these components in CNS vascular development and in specification of the blood-brain barrier (BBB) and blood-retina barrier (BRB). We determined that vasculature in various CNS regions is differentially sensitive to perturbations in canonical WNT signaling. The closely related WNT signaling coreceptors LDL receptor-related protein 5 (LRP5) and LRP6 had redundant functions in brain vascular development and barrier maintenance; however, loss of LRP5 alone dramatically altered development of the retinal vasculature. The BBB in the cerebellum and pons/interpeduncular nuclei was highly sensitive to decrements in canonical WNT signaling, and WNT signaling was required to maintain plasticity of barrier properties in mature CNS vasculature. Brain and retinal vascular defects resulting from ablation of Norrin/Frizzled4 signaling were ameliorated by stabilizing ß-catenin, while inhibition of ß-catenin-dependent transcription recapitulated the vascular development and barrier defects associated with loss of receptor, coreceptor, or ligand, indicating that Norrin/Frizzled4 signaling acts predominantly through ß-catenin-dependent transcriptional regulation. Together, these data strongly support a model in which identical or nearly identical canonical WNT signaling mechanisms mediate neural tube and retinal vascularization and maintain the BBB and BRB.


Subject(s)
Blood-Brain Barrier/physiology , Blood-Retinal Barrier/physiology , Neovascularization, Physiologic/physiology , Wnt Signaling Pathway/physiology , Animals , Eye Proteins/genetics , Frizzled Receptors/physiology , Low Density Lipoprotein Receptor-Related Protein-5/physiology , Low Density Lipoprotein Receptor-Related Protein-6/physiology , Mice , Nerve Tissue Proteins/genetics , Retina/physiology , Tamoxifen/analogs & derivatives , Tamoxifen/pharmacology , beta Catenin/physiology
16.
Neuron ; 81(1): 103-19, 2014 Jan 08.
Article in English | MEDLINE | ID: mdl-24411735

ABSTRACT

Female eutherian mammals use X chromosome inactivation (XCI) to epigenetically regulate gene expression from ∼4% of the genome. To quantitatively map the topography of XCI for defined cell types at single cell resolution, we have generated female mice that carry X-linked, Cre-activated, and nuclear-localized fluorescent reporters--GFP on one X chromosome and tdTomato on the other. Using these reporters in combination with different Cre drivers, we have defined the topographies of XCI mosaicism for multiple CNS cell types and of retinal vascular dysfunction in a model of Norrie disease. Depending on cell type, fluctuations in the XCI mosaic are observed over a wide range of spatial scales, from neighboring cells to left versus right sides of the body. These data imply a major role for XCI in generating female-specific, genetically directed, stochastic diversity in eutherian mammals on spatial scales that would be predicted to affect CNS function within and between individuals.


Subject(s)
Mosaicism , Nerve Tissue Proteins/metabolism , Neurons/metabolism , X Chromosome Inactivation/genetics , X Chromosome/genetics , Animals , Animals, Newborn , Brain/cytology , Brain/embryology , Brain/growth & development , Choline O-Acetyltransferase/metabolism , Embryo, Mammalian , Female , Functional Laterality/genetics , Gene Expression Regulation, Developmental/genetics , Green Fluorescent Proteins/genetics , Humans , Hypoxanthine Phosphoribosyltransferase/genetics , Mice , Mice, Transgenic , Neurons/classification , Polymorphism, Single Nucleotide/genetics , Retina/cytology
17.
Proc Natl Acad Sci U S A ; 110(40): E3830-9, 2013 Oct 01.
Article in English | MEDLINE | ID: mdl-24043815

ABSTRACT

Endothelin signaling is required for neural crest migration and homeostatic regulation of blood pressure. Here, we report that constitutive overexpression of Endothelin-2 (Edn2) in the mouse retina perturbs vascular development by inhibiting endothelial cell migration across the retinal surface and subsequent endothelial cell invasion into the retina. Developing endothelial cells exist in one of two states: tip cells at the growing front and stalk cells in the vascular plexus behind the front. This division of endothelial cell states is one of the central organizing principles of angiogenesis. In the developing retina, Edn2 overexpression leads to overproduction of endothelial tip cells by both morphologic and molecular criteria. Spatially localized overexpression of Edn2 produces a correspondingly localized endothelial response. Edn2 overexpression in the early embryo inhibits vascular development at midgestation, but Edn2 overexpression in developing skin and brain has no discernible effect on vascular structure. Inhibition of retinal angiogenesis by Edn2 requires expression of Endothelin receptor A but not Endothelin receptor B in the neural retina. Taken together, these observations imply that the neural retina responds to Edn2 by synthesizing one or more factors that promote the endothelial tip cell state and inhibit angiogenesis. The response to Edn2 is sufficiently potent that it overrides the activities of other homeostatic regulators of angiogenesis, such as Vegf.


Subject(s)
Angiogenesis Inhibitors/metabolism , Endothelial Cells/physiology , Endothelin-2/metabolism , Receptor, Endothelin A/metabolism , Retinal Vessels/embryology , Signal Transduction/physiology , Animals , Base Sequence , Endothelial Cells/cytology , Histocytochemistry , In Situ Hybridization , Mice , Microarray Analysis , Molecular Sequence Data , Retinal Vessels/metabolism , Sequence Analysis, RNA
18.
J Clin Invest ; 123(6): 2643-53, 2013 Jun.
Article in English | MEDLINE | ID: mdl-23676500

ABSTRACT

To explore the physiological functions of endothelin-2 (ET-2), we generated gene-targeted mouse models. Global Et2 knockout mice exhibited severe growth retardation and juvenile lethality. Despite normal milk intake, they suffered from internal starvation characterized by hypoglycemia, ketonemia, and increased levels of starvation-induced genes. Although ET-2 is abundantly expressed in the gastrointestinal tract, the intestine was morphologically and functionally normal. Moreover, intestinal epithelium-specific Et2 knockout mice showed no abnormalities in growth and survival. Global Et2 knockout mice were also profoundly hypothermic. Housing Et2 knockout mice in a warm environment significantly extended their median lifespan. However, neuron-specific Et2 knockout mice displayed a normal core body temperature. Low levels of Et2 mRNA were also detected in the lung, with transient increases soon after birth. The lungs of Et2 knockout mice showed emphysematous structural changes with an increase in total lung capacity, resulting in chronic hypoxemia, hypercapnia, and increased erythropoietin synthesis. Finally, systemically inducible ET-2 deficiency in neonatal and adult mice fully reproduced the phenotype previously observed in global Et2 knockout mice. Together, these findings reveal that ET-2 is critical for the growth and survival of postnatal mice and plays important roles in energy homeostasis, thermoregulation, and the maintenance of lung morphology and function.


Subject(s)
Endothelin-2/deficiency , Growth Disorders/genetics , Hypothermia/genetics , Pulmonary Emphysema/genetics , Animals , Blood Glucose , Body Temperature Regulation/genetics , Dietary Fats/metabolism , Endothelin-2/genetics , Energy Metabolism/genetics , Gene Expression , Genes, Lethal , Intestinal Absorption/genetics , Intestine, Small/metabolism , Intestine, Small/physiopathology , Lung/metabolism , Lung/pathology , Lung/physiopathology , Mice , Mice, Knockout , Organ Specificity , RNA, Messenger/genetics , RNA, Messenger/metabolism
19.
Elife ; 2: e00638, 2013 Mar 19.
Article in English | MEDLINE | ID: mdl-23538852

ABSTRACT

The use of the least squares method to calculate the best-fitting line through a two-dimensional scatter plot typically requires the user to assume that one of the variables depends on the other. However, in many cases the relationship between the two variables is more complex, and it is not valid to say that one variable is independent and the other is dependent. When analysing such data researchers should consider plotting the three regression lines that can be calculated for any two-dimensional scatter plot.


Subject(s)
Biomedical Research , Least-Squares Analysis , Regression Analysis
20.
Cell ; 151(6): 1332-44, 2012 Dec 07.
Article in English | MEDLINE | ID: mdl-23217714

ABSTRACT

Norrin/Frizzled4 (Fz4) signaling activates the canonical Wnt pathway to control retinal vascular development. Using genetically engineered mice, we show that precocious Norrin production leads to premature retinal vascular invasion and delayed Norrin production leads to characteristic defects in intraretinal vascular architecture. In genetic mosaics, wild-type endothelial cells (ECs) instruct neighboring Fz4(-/-) ECs to produce an architecturally normal mosaic vasculature, a cell nonautonomous effect. However, over the ensuing weeks, Fz4(-/-) ECs are selectively eliminated from the mosaic vasculature, implying the existence of a quality control program that targets defective ECs. In the adult retina and cerebellum, gain or loss of Norrin/Fz4 signaling results in a cell-autonomous gain or loss, respectively, of blood retina barrier and blood brain barrier function, indicating an ongoing requirement for Frizzled signaling in barrier maintenance and substantial plasticity in mature CNS vascular structure.


Subject(s)
Blood-Brain Barrier , Blood-Retinal Barrier , Endothelial Cells/metabolism , Eye Proteins/metabolism , Frizzled Receptors/metabolism , Nerve Tissue Proteins/metabolism , Retina/metabolism , Signal Transduction , Animals , Cell Movement , Cell Proliferation , Endothelial Cells/cytology , Eye Proteins/genetics , Frizzled Receptors/genetics , Humans , Mice , Mosaicism , Nerve Tissue Proteins/genetics , Neuronal Plasticity , Retina/cytology , Retina/embryology , Vascular Endothelial Growth Factor A/metabolism
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