Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 2 de 2
Filter
Add more filters










Database
Language
Publication year range
2.
Eur J Clin Microbiol Infect Dis ; 18(1): 35-41, 1999 Jan.
Article in English | MEDLINE | ID: mdl-10192712

ABSTRACT

Decision analysis methods were used to compare four mycobacteriology laboratory strategies with respect to time to confirmation and exclusion of smear-positive and smear-negative cases of pulmonary tuberculosis. Strategies assessed included the following: (i) polymerase chain reaction (PCR) on all respiratory specimens; (ii) PCR on smear-positive specimens and on the broth of vials for other specimens attaining a growth index >10 in a radiometric culture detection system; (iii) PCR on smear-positive specimens only; and (iv) radiometric culture detection, with DNA probe for species identification of vials attaining a growth index >999. Strategies i and ii had predicted average times to confirm cases of 5 and 7.6 days, respectively, and remained within 3 days of each other over a broad range of PCR performance with smear-negative specimens. In contrast, case-confirmation times using strategies iii and iv were 10.4 and 15.3 days, respectively. Only 10% of specimens were processed by PCR in strategy ii. Times to confirm smear-negative cases were comparable for strategies i and ii when PCR sensitivity was <40% with these specimens. Times to exclude pulmonary tuberculosis were similar for all strategies. Given the current suboptimal performance of PCR with smear-negative specimens, strategy ii offers accelerated case confirmation with limited PCR usage.


Subject(s)
Mycobacterium tuberculosis/genetics , Polymerase Chain Reaction , Tuberculosis, Pulmonary/diagnosis , Bacteriological Techniques , Clinical Laboratory Techniques , DNA, Bacterial/analysis , Decision Support Techniques , Humans , Sensitivity and Specificity
SELECTION OF CITATIONS
SEARCH DETAIL
...