Blastocystis in captivated and free-ranging wild animals worldwide: a review.

Blastocystis is the most prevalent eukaryotic gastrointestinal symbiont found in humans and animals worldwide. Increased human infection rates are associated with raising concerns about the involvement of the parasite in public health. Over the last decade, the number of linked epidemiology studies has been prudently grown. Microscopy has been used to detect the presence of protozoan and the advent of molecular techniques has made detection easier. However, due to its limited host specificity and zoonotic potential, animals, either livestock or wildlife animals, may serve as a potential reservoir for Blastocystis infection transmission. The approach utilised in this study aided in understanding the distribution and prevalence of Blastocystis in animals, particularly captivated and free-ranging wild animals worldwide due to increased interest. This review will help comprehend the epidemiological aspects, demographic, subtypes, and the zoonotic potential of Blastocystis in wildlife and captive animals.

Prevalence of Blastocystis sp. in freshwater fishes, poultry, and caprine from Penang, Malaysia.

Blastocystis is an enigmatic intestinal protist of humans and many animals. There is growing interest in its potential as an enteric pathogen, and the possible role of domestic and in-contact animals as reservoirs for human infection. The purpose of this study was to determine the prevalence of Blastocystis infection in fishes, poultry, and caprine in Penang, Malaysia. A total of 353 faecal samples/intestinal contents, consisting of 123 intestinal contents from freshwater fishes, 96 intestinal contents of commercially barn-reared chickens, 84 intestinal contents of barn-reared quails, and 50 faecal samples of caprine (29 from meat goats and 21 from dairy goats) were collected. Faecal sample/intestinal content from each animal was subjected to in-vitro cultivation method using Jones' medium supplemented with 10% horse serum. The respective colonization frequencies for dairy and meat goats were 47.6% and 31.0%, whereas 26.1% was recorded for chickens and 16.7% for quails. None of the freshwater fishes were found infected with Blastocystis. The organism was most commonly seen as spherically shaped vacuolated forms and cell diameter was significantly larger in poultry than in caprine. For further studies, molecular characterization of Blastocystis in poultry and livestock animals in the study area is highly recommended.

Natural infection ofBlastocystis ST6 among commercial quails (Coturnix coturnix) in Penang, Malaysia.

Blastocystis sp. is a unicellular, anaerobic intestinal protist regularly reported in humans and various animals worldwide. There seems to be little research on Blastocystis infection in poultry in Malaysia, and none on Blastocystis in quail specifically. In Malaysia, the consumption of quail meat and eggs is rapidly gaining popularity as a significant source of protein. It is, therefore, essential to explore the presence of Blastocystis in Malaysian quails in order to aid in the understanding of Blastocystis in this group of birds and their role in its transmission. Intestinal contents were collected from 90 commercial quails raised on two farms in Penang, Malaysia, in a multi-layer cage system with adequate farm management. Detection of Blastocystis sp. was by cultivation in modified Jones' medium supplemented with 10% horse serum. Giemsa-stained slides made from positive cultures were used for morphological studies whereas Blastocystis subtyping was conducted by using Polymerase Chain Reaction (PCR). A prevalence of 17.8% (16/90) was recorded for Blastocystis sp. in quail in this study. The most common forms detected in the in vitro culture medium were vacuolar and granular forms with cell diameters ranging from 9.09 µm to 33.33 µm. None of the quail birds screened had any visible gastrointestinal symptoms or signs. All successfully sequenced isolates were identified as Blastocystis sp. ST6, one of the potentially zoonotic subtypes of Blastocystis. This study posits that the quail birds may serve as reservoirs of zoonotic subtypes of Blastocystis. More studies are required to understand the source of Blastocystis infection to poultry under intensive care and the role of poultry animals in the transmission of Blastocystis to humans.

Natural infection of Blastocystis ST6 among commercial quails (Coturnix coturnix) in Penang, Malaysia

@#Blastocystis sp. is a unicellular, anaerobic intestinal protist regularly reported in humans and various animals worldwide. There seems to be little research on Blastocystis infection in poultry in Malaysia, and none on Blastocystis in quail specifically. In Malaysia, the consumption of quail meat and eggs is rapidly gaining popularity as a significant source of protein. It is, therefore, essential to explore the presence of Blastocystis in Malaysian quails in order to aid in the understanding of Blastocystis in this group of birds and their role in its transmission. Intestinal contents were collected from 90 commercial quails raised on two farms in Penang, Malaysia, in a multi-layer cage system with adequate farm management. Detection of Blastocystis sp. was by cultivation in modified Jones’ medium supplemented with 10% horse serum. Giemsa-stained slides made from positive cultures were used for morphological studies whereas Blastocystis subtyping was conducted by using Polymerase Chain Reaction (PCR). A prevalence of 17.8% (16/90) was recorded for Blastocystis sp. in quail in this study. The most common forms detected in the in vitro culture medium were vacuolar and granular forms with cell diameters ranging from 9.09μm to 33.33μm. None of the quail birds screened had any visible gastrointestinal symptoms or signs. All successfully sequenced isolates were identified as Blastocystis sp. ST6, one of the potentially zoonotic subtypes of Blastocystis. This study posits that the quail birds may serve as reservoirs of zoonotic subtypes of Blastocystis. More studies are required to understand the source of Blastocystis infection to poultry under intensive care and the role of poultry animals in the transmission of Blastocystis to humans.

Blastocystis in captivated and free-ranging wild animals worldwide: a review

@#Blastocystis is the most prevalent eukaryotic gastrointestinal symbiont found in humans and animals worldwide. Increased human infection rates are associated with raising concerns about the involvement of the parasite in public health. Over the last decade, the number of linked epidemiology studies has been prudently grown. Microscopy has been used to detect the presence of protozoan and the advent of molecular techniques has made detection easier. However, due to its limited host specificity and zoonotic potential, animals, either livestock or wildlife animals, may serve as a potential reservoir for Blastocystis infection transmission. The approach utilised in this study aided in understanding the distribution and prevalence of Blastocystis in animals, particularly captivated and free-ranging wild animals worldwide due to increased interest. This review will help comprehend the epidemiological aspects, demographic, subtypes, and the zoonotic potential of Blastocystis in wildlife and captive animals.

Molecular subtyping and phylogeny of Blastocystis sp. isolated from turkey (Meleagris gallopavo) populations in Penang, Malaysi.

Most poultry farms in Malaysia preferred rearing chickens either for eggs or/and meat than turkeys. This is due to several challenges such as parasitic load and heat stress in rearing turkey. Blastocystis is one of the most common protozoan parasites infecting poultry. As no study was conducted on Blastocystis infection in turkey in Malaysia, this study aims to determine the current status, the morphological characteristics and subtyping of Blastocystis from turkey reared either in closed house or free-range system in Penang, Malaysia. It was found that the prevalence of Blastocystis sp. infection in turkeys were moderately high with 41.6% (25/60) in the closed house and 45.0% (45/100) in free-range system as infection was higher in the female turkeys with no gastrointestinal signs and symptoms. Vacuolar form was the most common form found in the in vitro culture ranged between 5 to 20 µm in diameter with a rough surface coat and undulating cell surface viewed under the scanning electron microscope. Meanwhile, the ultrastructure of the cells from turkey isolates were varies with partially expanded electron-opaque vacuoles to electron-dense in fully distended vacuoles. Interestingly, sequence analysis for 30 positive Blastocystis isolates from turkeys revealed one subtypes with three alleles namely, ST7 allele 99 (73.4%, n=22), ST7 allele 100 (23.3%, n=7) and ST7 allele 101 (3.3%, n=1). Findings from this study added to our understanding on Blastocystis infection in turkey production.

Molecular subtyping and phylogeny of Blastocystis sp. isolated from turkey (Meleagris gallopavo) populations in Penang, Malaysia

@#Most poultry farms in Malaysia preferred rearing chickens either for eggs or/and meat than turkeys. This is due to several challenges such as parasitic load and heat stress in rearing turkey. Blastocystis is one of the most common protozoan parasites infecting poultry. As no study was conducted on Blastocystis infection in turkey in Malaysia, this study aims to determine the current status, the morphological characteristics and subtyping of Blastocystis from turkey reared either in closed house or free-range system in Penang, Malaysia. It was found that the prevalence of Blastocystis sp. infection in turkeys were moderately high with 41.6% (25/60) in the closed house and 45.0% (45/100) in free-range system as infection was higher in the female turkeys with no gastrointestinal signs and symptoms. Vacuolar form was the most common form found in the in vitro culture ranged between 5 to 20 μm in diameter with a rough surface coat and undulating cell surface viewed under the scanning electron microscope. Meanwhile, the ultrastructure of the cells from turkey isolates were varies with partially expanded electron-opaque vacuoles to electron-dense in fully distended vacuoles. Interestingly, sequence analysis for 30 positive Blastocystis isolates from turkeys revealed one subtypes with three alleles namely, ST7 allele 99 (73.4%, n=22), ST7 allele 100 (23.3%, n=7) and ST7 allele 101 (3.3%, n=1). Findings from this study added to our understanding on Blastocystis infection in turkey production.