ABSTRACT
The DNA synthesis, the cytolytic activity, and the ultrastructure of cytolytic T lymphocytes (CTL) derived from mixed mouse thymocyte culture on day 5 were studied. CTL of thymic origin were absorbed by centrifugation on the surface of target cell (TC) monolayers. At different time intervals after absorption, single tubular structures (TS) and complex of tubular structures (CTS) linked with ergastoplasmic reticulum, secretory granules, Golgi apparatus, coated vesicles, and multivesicular bodies were detected in the CTL cytoplasm. The linkage of CTS with ergastoplasmic reticulum, ribosomes, and secretory lymphocyte mechanism suggests the possibility of a secretory-receptor mechanism of TC cytolysis. The release of numerous secretory vacuoles was accompanied by the enlargement of the cytoplasmatic lymphocyte membrane surface, which seemed to cause its shedding. 'Membranosomas' were observed on CTL membrane; their role is still obscure and awaits further study.
Subject(s)
Intracellular Membranes/ultrastructure , T-Lymphocytes, Cytotoxic/ultrastructure , Animals , Cytoplasm/ultrastructure , Endoplasmic Reticulum/ultrastructure , Lymphocyte Culture Test, Mixed , Mice , Mice, Inbred BALB C , Mice, Inbred C3H , Microscopy, Electron , Spleen/cytology , Thymus Gland/cytologySubject(s)
Cytoplasmic Granules/ultrastructure , Cytotoxicity, Immunologic , Organoids/ultrastructure , T-Lymphocytes/ultrastructure , Vacuoles/ultrastructure , Animals , Cytoplasmic Granules/metabolism , Lipids , Lysosomes/ultrastructure , Mice , Mice, Inbred C3H , Mice, Inbred DBA , Mitochondria/ultrastructureSubject(s)
Cytotoxicity, Immunologic , Lymphocyte Activation , Lymphocytes/immunology , Vitamin B 6 Deficiency/immunology , Animals , Cytotoxicity, Immunologic/drug effects , Female , Histocompatibility Antigens , Lymphocyte Activation/drug effects , Lymphocyte Culture Test, Mixed , Mice , Pyridoxine/pharmacology , Time FactorsABSTRACT
Blastomogenic activity of p-hydroxyphenyllactic acid was established by prolonged s.c. administration into C57BL/6 and CC57BR mice. Phenyllactic acid was also tested and was not blastomogenic under the same conditions. The preparations in aqueous solutions were injected into the dorsal region twice weekly in 1.5-mg doses. The total dose per mouse was 42 mg p-hydroxyphenyllactic acid or 50 mg phenyllactic acid. The resorptive character and many forms of blastomogenic activity of p-hydroxphenyllactic acid were shown. It induced neoplasms of varied histogenesis (leukemias, adenomas, hepatomas, and tumors of the vascular tissue),including benign and malignant tumors and precancerous conditions of the urniary bladder.
