Anti-fouling potential and in-silico analysis of carotenoid and fatty acids from Rauvolfia tetraphylla L.

Study investigated the antifouling potential ofRauvolfia tetraphyllaL. fruit, leaf and stem extracts against the marine fouling organisms throughin-vitroand in-silicoapproach. Methanolic crude extract of R. tetraphylla L.leaf exhibited maximum antibacterial potential against six fouling organisms isolated from Parangipettai coast and was further taken up for column fractionation. Twenty-four fractions were obtained, among which five fractions showed inhibitory efficiency against microfoulers of Bacillus megaterium. The active compounds present in the bioactive fraction were identified by FTIR, GC-MS and NMR (13C; 1H). The bioactive compounds that exhibited maximum antifouling activity were identified as Lycopersene (80%), Hexadecanoic acid; 1, 2-Benzenedicarboxylic acid, dioctyl ester; Heptadecene - (8) - carbonic acid - (1) and Oleic acid. Molecular docking studies of the potent anti-fouling compounds Lycopersene, Hexadecanoic acid, 1, 2-Benzenedicarboxylic acid, dioctyl ester and Oleic acid showed the binding energy of 6.6, - 3.8, -5.3 and -5.9 (Kcal/mol) and hence these compounds will act as a potential biocide to control the aquatic foulers. Moreover, further studies need to carry out in terms of toxicity, field assessment and clinical trial in order to take these biocides for a patent.

Lycopersene: A review on extraction, identification and purification and applications.

Lycopersene is a stable and safe triterpenoid. Lycopersen had utilized as an antioxidant, antimutagenic, antiproliferative, cytotoxicity, antibacterial and pesticide. Obtaining pure Lycopersene from natural sources is very important for fundamental research and the above applications. The present overview provides an up-to-date and comprehensive summary of the various methods used for extraction, isolation and purification of Lycopersene from natural sources with its applications in food, cosmetics and pharmaceutical fields. Many different extraction methods ranging from conventional techniques (e.g. Soxhlet extraction, Maceration and Rotary evaporator) and other advanced extraction technologies (e.g. Solid-phase microextraction, steam distillation with Clevenger, Clevenger apparatus, Chromatography on SiO2 column, centrifuge, sonication) had been used to obtain Lycopersene from flora and fauna. Purification techniques, alone or in combination, have been investigated for isolation and purification of Lycopersene from crude extracts in various natural sources. The review of Lycopersene identified cap areas like purity and application in various fields.