Subject(s)
Anemia, Hemolytic, Autoimmune/chemically induced , Antibiotic Prophylaxis/adverse effects , Cefotetan/adverse effects , Cephamycins/adverse effects , Anemia, Hemolytic, Autoimmune/blood , Anemia, Hemolytic, Autoimmune/therapy , Cholecystectomy , Erythrocyte Transfusion , Female , Hemoglobins/analysis , Herniorrhaphy , Humans , Middle Aged , Time FactorsABSTRACT
The workshop on "Correlations of Aging and Space Effects on Biosystems" was held to explore the possibility that research in the space environment would be useful to the understanding of some phenomena of aging on Earth and that life sciences in space would benefit from this collaboration. The genesis and purpose of this workshop and the rationale for the concept are presented below. The unique characteristics of the space environment are described as background for discussion of the utility of this approach and the author's belief that carefully thought out selected projects would be of mutual benefit to the space and aging research communities.
Subject(s)
Aging , Space Flight , HumansSubject(s)
Antiviral Agents/therapeutic use , Deoxy Sugars/therapeutic use , Deoxyglucose/therapeutic use , Herpes Simplex/drug therapy , Keratitis, Dendritic/drug therapy , Animals , Antiviral Agents/pharmacology , Culture Techniques , Deoxyglucose/pharmacology , Female , Genital Diseases, Female/drug therapy , Glycolysis/drug effects , Humans , Rabbits , Simplexvirus/drug effects , Simplexvirus/metabolismSubject(s)
Ceramides/biosynthesis , Deoxy Sugars/pharmacology , Deoxyglucose/pharmacology , G(M3) Ganglioside/biosynthesis , Gangliosides/biosynthesis , Glycosphingolipids/biosynthesis , Simplexvirus , Cell Line , Glucosyltransferases/metabolism , Microsomes/enzymology , Palmitates/metabolism , Serine/metabolism , Uridine Diphosphate GlucoseABSTRACT
Human high density lipoprotein enriched in free cholesterol was obtained by exposing the lipoprotein to lipid dispersions having a free cholesterol/lecithin molar ratio greater than two. The metabolism of cholesterol was studied in tissue culture cells exposed to normal and cholesterol-enriched lipoproteins. Incubation of Fu5-AH rat hepatoma cells in medium containing cholesterol-enriched lipoprotein resulted in the accumulation of cellular cholesterol whereas normal high density lipoprotein produced no change in cellular content. The accumulated sterol was recovered primarily as esterified cholesterol and was derived almost entirely from lipoprotein free cholesterol. The esterification of incorporated free cholesterol and the cellular cholesterol content were directly related to the molar ratio of free cholesterol to phospholipid in the lipoprotein and to the concentration of lipoprotein in the culture medium. Isotopic experiments utilizing lipoprotein labeled with 125I or [4-14C]cholesteryl oleate demonstrated that a large fraction of the cholesterol incorporated from lipoprotein enriched in free cholesterol occurred by mechanisms that did not result in lipoprotein internalization and degradation. The response of other tissue culture cells to cholesterol/phospholipid dispersions is presented. The data indicate that the lipid composition of a lipoprotein can regulate free cholesterol uptake and esterification as well as cellular cholesterol content.
