Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 6 de 6
Filter
Add more filters










Database
Language
Publication year range
2.
Exp Gerontol ; 26(2-3): 123-9, 1991.
Article in English | MEDLINE | ID: mdl-1680731

ABSTRACT

The workshop on "Correlations of Aging and Space Effects on Biosystems" was held to explore the possibility that research in the space environment would be useful to the understanding of some phenomena of aging on Earth and that life sciences in space would benefit from this collaboration. The genesis and purpose of this workshop and the rationale for the concept are presented below. The unique characteristics of the space environment are described as background for discussion of the utility of this approach and the author's belief that carefully thought out selected projects would be of mutual benefit to the space and aging research communities.


Subject(s)
Aging , Space Flight , Humans
5.
J Lipid Res ; 19(3): 350-8, 1978 Mar.
Article in English | MEDLINE | ID: mdl-206640

ABSTRACT

Human high density lipoprotein enriched in free cholesterol was obtained by exposing the lipoprotein to lipid dispersions having a free cholesterol/lecithin molar ratio greater than two. The metabolism of cholesterol was studied in tissue culture cells exposed to normal and cholesterol-enriched lipoproteins. Incubation of Fu5-AH rat hepatoma cells in medium containing cholesterol-enriched lipoprotein resulted in the accumulation of cellular cholesterol whereas normal high density lipoprotein produced no change in cellular content. The accumulated sterol was recovered primarily as esterified cholesterol and was derived almost entirely from lipoprotein free cholesterol. The esterification of incorporated free cholesterol and the cellular cholesterol content were directly related to the molar ratio of free cholesterol to phospholipid in the lipoprotein and to the concentration of lipoprotein in the culture medium. Isotopic experiments utilizing lipoprotein labeled with 125I or [4-14C]cholesteryl oleate demonstrated that a large fraction of the cholesterol incorporated from lipoprotein enriched in free cholesterol occurred by mechanisms that did not result in lipoprotein internalization and degradation. The response of other tissue culture cells to cholesterol/phospholipid dispersions is presented. The data indicate that the lipid composition of a lipoprotein can regulate free cholesterol uptake and esterification as well as cellular cholesterol content.


Subject(s)
Cholesterol Esters/biosynthesis , Cholesterol/metabolism , Lipoproteins, HDL/metabolism , Animals , Aorta/metabolism , Carcinoma, Hepatocellular/metabolism , Cells, Cultured , Fibroblasts/metabolism , Humans , Liver/metabolism , Liver Neoplasms , Phosphatidylcholines/metabolism , Rats
SELECTION OF CITATIONS
SEARCH DETAIL
...