Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 1 de 1
Filter
Add more filters










Database
Language
Publication year range
1.
J Immunol Methods ; 288(1-2): 91-8, 2004 May.
Article in English | MEDLINE | ID: mdl-15183088

ABSTRACT

We have developed a novel method of high-throughput Multiplexed Competitive Antibody Binning (MCAB). Using only a small amount of antibody and antigen, this method enables the sorting of a large, complex panel of monoclonal antibodies into different bins based on cross-competition for antigen binding. The MCAB assay builds on Luminex multiplexing bead-based technology to detect antibody competition. Because of its high sensitivity, the MCAB method is immediately applicable after identification of antigen-positive mAbs, providing information useful for advancing mAb candidates into further testing. The MCAB assay also can be used for sorting mAbs into binding groups after screening for functional activity.


Subject(s)
Antibodies, Monoclonal/analysis , Antibodies, Monoclonal/immunology , Binding, Competitive/immunology , Animals , Binding Sites, Antibody/immunology , Epitope Mapping , Epitopes/immunology , Humans , Mice
SELECTION OF CITATIONS
SEARCH DETAIL
...