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Background The Sapindus mukorossi (SM) extract has been reported to possess antibacterial, antifungal, anti-inflammatory, and antioxidant characteristics. However, there is limited research demonstrating the effectiveness of SM in dissolving dental pulp tissue. Methods In an in vitro investigation, pulp tissue samples were extracted from human teeth, collectively weighing 144 mg. These samples were divided equally and activated by manual digital agitation (MDA) or ultrasonic (US) irrigation for three 30-second cycles with a resting period of 45 seconds between each activation. The samples in each group were sub-categorized into a set of three groups based on the treatment received as normal saline (NS), 5.25% sodium hypochlorite (Hypo), or Sapindus mukorossi (SM). Statistical tests, including the student t-test and one-way analysis of variance (ANOVA), were employed to compare the mean weight differences among the groups, with a significance level set at p ≤ 0.05 for all comparisons. Results The one-way analysis of variance (ANOVA) and independent t-test revealed significant intergroup differences (p<0.05). Turkey's post hoc analysis indicated significant distinctions, particularly when comparing Hypo with the other two irrigants, namely Hypo-NS (p<0.05) and Hypo-SM (p<0.05) when the MDA method was employed. Considering only the method adopted, the US technique was significantly superior (p=0.04) to the MDA. Conclusion Sapindus mukorossi (SM) demonstrated efficacy in dissolving pulp tissue but was not as effective as sodium hypochlorite (Hypo) which is the standard agent for root canal irrigation. SM to be used as an alternative to Hypo on clinical grounds needs further validation from research.
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AIM: The aim of this in vitro study was to evaluate the fracture resistance of anterior crown fragments using three different preparation designs and reattached using two different nanocomposites. MATERIALS AND METHODS: A total of 120 sound human permanent incisors were selected and divided into six groups. The teeth were sectioned and reattached using different preparatory designs and nanocomposites. Group A (simple reattachment with G-aenial Universal Flo), Group A2 (bevel preparation reattached with G-aenial Universal Flo), Group A3 (internal groove preparation reattached with G-aenial Universal Flo), Group B1 (simple reattachment with Polofil NHT Flow), Group B2 (bevel preparation reattached with Polofil NHT Flow), and Group B3 (internal groove preparation reattached with Polofil NHT Flow). The teeth were sectioned and reattached using different preparatory designs and nanocomposites. These teeth were then subjected to thermocycling, and fracture resistance of the reattached fragments was recorded using Instron machine. RESULTS: Group A3 showed the highest fracture resistance. The least fracture resistance was seen in Group B1. CONCLUSION: The fracture resistance of reattached incisor fragments depends mainly on the preparation design incorporated and also the material used to restore. Fragments where internal groove preparatory design was done and reattached with G-aenial Universal Flo, showed greater fracture resistance. Simple reattachment preparatory design showed the least fracture resistance, and hence must be avoided.
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BACKGROUND: The success in endodontic therapy is dependent largely on the absolute elimination of pulp tissue remnants and the maximum reduction of pathologic microbial load present within the root canal system. AIM: The aim of this in vitro study was to compare and evaluate the ability of pulp tissue dissolution with and without activation of 5.25% sodium hypochlorite (NaOCl). MATERIALS AND METHODS: Pulp tissue samples collected from intact, noncarious extracted third molars were standardized to 8 mg in each group. These samples were placed in conical Eppendorf tubes of 15 ml with 10 ml 5.25% NaOCl for 4 min and were activated suitably according to the group specifications. All the procedures in this study were carried out at room temperature. The preweighed pulp tissue samples were further weighed again after the dissolution phase, and residual weight loss in each group along with its percentage was obtained. The results were then statistically analyzed using one-way analysis of variance and Tukey post hoc test. RESULTS: The maximum amount of pulp tissue dissolution was found in the laser-assisted irrigation group, i.e., NaOCl with Er, Cr: YSGG (P < 0.05) and showed a statistically significant result in comparison with other groups. CONCLUSION: Within the limitations of the study, laser-assisted irrigation with a radial firing tip (NaOCl activated by Er, Cr: YSGG) was the most effective in dissolving the pulp tissue in comparison with other groups. The ultrasonic activation also showed a considerable amount of pulp tissue dissolution comparable to laser-activated irrigation in this study.
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AIM: To determine the degree of association between visual and tactile methods of caries removal compared with caries detector dye and laser fluorescence device (DIAGNOdent), which detects the degree of demineralization; to determine the presence of Streptococcus mutans via culture and polymerase chain reaction (PCR) techniques; and to find a suitable method for caries removal. MATERIALS AND METHODS: A total of 75 patients were divided into three groups: visual and tactile (Group A), visual and tactile with caries detector dye (Group B), and visual and tactile with caries detector dye along with laser florescence readings (Group C). Caries removal was carried out using visual and tactile methods, caries detector dye, and laser fluorescence, and the samples obtained were subjected to culture and PCR. The data obtained were statistically analyzed using Pearson's chi-square test, analysis of variance (ANOVA), and Tukey's post hoc test. RESULTS: Visual and tactile along with caries detector dye and laser florescence (Group C) is the most efficient method for caries removal. CONCLUSION: Caries detector dye along with visual, tactile examination and laser fluorescence is a valuable and superior tool for clinicians that aids in better caries removal and can prevent the overzealous removal of tooth structure.
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AIM: To evaluate the shear bond strength and failure mode of total-etch and self-etch bonding agents on human dentin with and without application of 2% chlorhexidine gluconate (CHX). MATERIALS AND METHODS: Eighty extracted premolars were chosen and stored in 10% formalin until use. Samples were mounted in cold cure acrylic resin and the occlusal enamel perpendicular to long axis of each tooth was removed using a low-speed diamond saw under water coolant. The prepared teeth were randomly divided into four groups of 20 samples each according to the adhesive system used. Groups 1 and 2 were the control group in which total-etch and self-etch adhesives were applied as per manufacturer's instructions, Groups 3 and 4 were the experimental groups in which 2% CHX was applied and blot dried prior to the application of total-etch and self-etch adhesives. A custom-designed rig was fabricated to place composite on samples. The customized rig comprised a cylindrical mold with height of 3 mm and internal diameter of 2.5 mm. Resin was placed in increments of 1 mm and was cured after each increments. After the composite placements, samples were placed in distilled water at 37°C for 24 h. The samples were then thermocycled between 5°C and 55°C in water with a dwell time of 30 s in each temperature to a total of 10,000 cycles. The shear test was performed using universal testing machine and fracture modes were evaluated using stereomicroscope. RESULTS: Both the experimental total-etch and self-etch groups showed better shear bond strength than the control groups, which was statistically significant, and also the least mode of failure at the adhesive interface was observed in both the experimental groups. CONCLUSION: 2% Chlorhexidine gluconate effectively improved the shear bond strength and helped maintaining the durability of adhesive interface in both total-etch and self-etch adhesives.
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AIM: The aim is to recycle mercury from dental amalgam scrap using the vacuum distillation method. MATERIALS AND METHODS: A total of 150 g of dental amalgam scrap was taken in a round bottom flask and was subjected to vacuum distillation at 398°C. The vapor of mercury was collected in another round bottom flask. OBSERVATION: The procedure is carried out for mercury recovery using vacuum distillation apparatus, and mercury vapor are collected in a round bottom flask, and the silver is recovered using sucrose as reducing agent. Using 150 g of dental amalgam scrap 50%-80% of silver are recovered, and silver has a purity of 70%-80%. However, the total time required in the reduction process ranged between 303 and 600 min. CONCLUSION: Mercury could be recycled from dental amalgam scrap through vacuum distillation method at 398°C and its implication of dental amalgam scrap in an Indian perspective.
