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Folia Microbiol (Praha) ; 48(4): 549-53, 2003.
Article in English | MEDLINE | ID: mdl-14533489

ABSTRACT

Enzyme immunoassay (EIA) using recombinant antigens for the detection of Treponema pallidum-specific antibodies in sera of syphilis patients was developed. Four low-molar-mass Treponema antigens (Tp15, Tp17, TmpA, Tp47) were investigated; 17- and 47-kDa proteins were demonstrated as immunodominant as they permitted to obtain the most sensitive EIA. Using a mixture of these proteins a 3rd-generation-EIA kit Dia-Syph was constructed, its sensitivity being 99.4% during tests of 165 sera of syphilitic patients. No false result was obtained on the commercial panel PSS01 (BBI, USA). The specificity of the elaborated test system (99.7%) was determined on 295 sera.


Subject(s)
Antibodies, Bacterial/blood , Antigens, Bacterial/immunology , Enzyme-Linked Immunosorbent Assay , Syphilis Serodiagnosis , Treponema pallidum/immunology , Antigens, Bacterial/genetics , Antigens, Bacterial/isolation & purification , Humans , Recombinant Proteins/immunology , Recombinant Proteins/isolation & purification , Sensitivity and Specificity , Treponema pallidum/genetics , Treponema pallidum/metabolism
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