Comprehensive assessment of memory function, inhibitory control, neural activity, and cortisol levels in late pregnancy.

A considerable proportion of women subjectively perceive a detriment to their cognitive capacity during pregnancy, with decreased memory functions being the most frequently self-reported concerns. However, objective investigation of these perceived cognitive deficits has yielded inconsistent results. This study focused on memory functions during late pregnancy using multiple tasks designed to assess various memory indices, for example, working memory, learning rate, immediate recall, proactive and retroactive interference, delayed recall, retrieval efficiency, visuospatial constructional ability, recognition, and executive function. Additionally, sustained attention and inhibitory control were examined using a combined recognition stop-signal task. Electrophysiological brain activity during this task was recorded using a 128-channel electroencephalographic-event-related potential system. Salivary cortisol levels were assessed both prior to and following the experimental session. In contrast to the widely held belief, results demonstrated that women in late pregnancy did not exhibit a decline in their performance across the various memory tests. In terms of accuracy, there was not a single task in which poorer performance was found for pregnant women. The quality of memory performance was comparable, and in some cases even superior, among women in the pregnancy group. On the stop-signal task, pregnant women exhibited significantly better performance, and their electrophysiological data revealed greater centrally distributed P300 amplitude to "stop" signs, which may signify an enhanced neural efficiency in the domains of inhibitory executive control. Endocrine results revealed that pregnant women exhibited significantly lower levels of salivary cortisol, suggesting an attenuation of hypothalamic-pituitary-adrenocortical axis activity, which may contribute to the optimization of fetal development and growth.

Physiological indicators of emotional arousal related to ANS activity in response to associative cards for psychotherapeutic PTSD treatment.

SEE FAR CBT is an integrative treatment protocol for PTSD and anxiety disorders which combines CBT, body-mind (somatic experience) and imagery-based (fantastic reality; FR) methods. FR is introduced using associative therapeutic cards (COPE cards) to represent both "a pleasant/safe place" and the re-narrating process of the traumatic story. Although some preliminary evidence exists regarding the impact of COPE cards integration in psychotherapy, further validation is needed as to whether these cards can induce distinct arousal-affective states in the observer. The aim of this study was to examine whether exposure to COPE cards evoke different emotional-psychophysiological states using objective physiological measures reflecting autonomic nervous system responses; hence, to further validate its use as a potentially effective tool within the context of SEE FAR CBT therapeutic process. Ninety-five healthy under-graduate participants were first exposed to high-arousal, negatively-valenced cards and asked to put themselves in a state of emotional/physical arousal. Afterwards, they were exposed to low-arousal, positively-valenced cards and were asked to try to calm and relax to the best of their ability. Heart rate, blood pressure and heart rate variability (HRV) were measured at baseline, at the arousal phase and finally at the relaxation phase. It was found that exposure to arousing negative cards resulted in significant increase in blood pressure and a decrease in HRV, while exposure to relaxing positive cards resulted in significant decrease in blood pressure and an increase in HRV. These findings support the efficacy and utility of associative COPE cards in affecting psychophysiological arousal.

Associations between red blood cell indices and iron status and neurocognitive function in young adults: Evidence from memory and executive function tests and event-related potentials.

Cognitive impairment has been associated with anemia and iron deficiency; however, brain electrophysiological studies correlating red blood cell (RBC) indices and iron status to cognition in adulthood are scarce. We aimed to assess neurocognitive function in young adults of the general population and its correlation with RBC indices and iron status. Neurocognitive function was investigated using scalp-recorded event-related potentials (ERPs) within the context of a task-switching paradigm. ERPs and test performance were also compared across groups of "high"/"low" RBC and iron indices. Working memory was examined using the digit span test, in which mean corpuscular hemoglobin (MCH), mean corpuscular volume (MCV), and ferritin were found to be significant predictors of test performance, with higher MCH/MCV/ferritin being associated with better test scores. In the switching task, MCH, MCV, and ferritin were found to be significant predictors of task performance, with higher MCH/MCV/ferritin levels associated with a lower percentage of errors. Electrophysiological results showed that MCH and MCV were significant predictors of ERPs amplitude, with lower MCH/MCV levels associated with greater amplitude, which may reflect compensatory processes. P1, N1, P2, and P3 were greater for the low MCH/MCV groups. This is the first evidence of association between levels of MCH/MCV and brain function while engaged in an executive function task; possibly reflecting brain iron availability.

The relationship between individuals with fearful-avoidant adult attachment orientation and early neural responses to emotional content: An event-related potentials (ERPs) study.

OBJECTIVE: Adult attachment orientation affects the interactions of individuals with others. Fearful-avoidant individuals are characterized by both high anxiety and high avoidance. This study examines the response of individuals with fearful-avoidant attachment orientation to neutral, emotionally positive, or emotionally negative visual stimuli. METHOD: Participants included 119 undergraduate students (91 female), mean age 23.5 ± 3.6 years: 30 fearful-avoidant, 32 secure, 28 anxious-preoccupied, and 29 dismissive-avoidant. The response of individuals with fearful-avoidant attachment orientation to IAPS emotional and neutral pictures was measured using Event-Related Potential (ERP), and compared to individuals with dismissive-avoidance or anxious-preoccupied attachment orientations (with a secure attachment group as control). The study focused on early time reaction, as defined by the P1, N1, and P2 components (110-165, 165-215, and 215-280 ms poststimulus, respectively). RESULTS: We find that individuals with fearful-avoidant attachment orientation display a reaction to emotional (positive or negative) cues, at the P1 and P2 components, that is significantly different than that of individuals with anxious-preoccupied attachment, but is similar to that of individuals with dismissive-avoidant attachment. CONCLUSIONS: Our results suggest that the initial response to emotional stimuli of individuals with fearful-avoidant attachment is dominated by avoidance, rather than anxiety. (PsycINFO Database Record (c) 2020 APA, all rights reserved).

Cognitive, neural and endocrine functioning during late pregnancy: An Event-Related Potentials study.

This study investigated cognitive, neural and endocrine function during late pregnancy. One of the first to examine brain ERPs in pregnant women, the study is unique in its focus on response inhibition function. In the study, cognitive function was evaluated by a digit-symbol coding test, an arithmetic ability test, and a visual stop-signal task which places enhanced demands on impulse control and response inhibition, considered a hallmark of executive function. Brain activity was measured by scalp-recorded Event-Related Potentials (ERPs) during the stop-signal task. HPA axis reactivity was assessed by measuring salivary cortisol levels before and after experimental sessions. Test performance, ERPs and cortisol reactivity were compared across groups of 23 women in their third trimester of pregnancy and 22 non-pregnant controls. Pregnant women scored lower than the control group on the digit-symbol coding test. On the stop-signal task, both groups had similar error rates, but pregnant women had longer response times to Go trials. On the Stop condition of the task in which a response must be inhibited, pregnant women demonstrated significantly better performance. At the electrophysiological level, in response to Go stimuli pregnant women exhibited greater amplitude of P2 than controls. In response to Stop-signals, pregnant women had lesser amplitudes of P1 and N2 and greater amplitude of P3. Cortisol reactivity to the test session was significantly more pronounced in non-pregnant women with significant correlations found between cortisol reactivity and behavioral responses. The results suggest that response patterns of women in late pregnancy are less impulsive and more cautious and controlled.

Attention, response inhibition and brain event-related potential alterations in adults with beta-thalassaemia major.

We investigated neural correlates of cognitive function in adults with beta thalassaemia major (ß-TM) compared to healthy controls using scalp-recorded event-related potentials (ERPs). Event-related potential studies in the field of ß-TM are scarce and mostly limited to children. A stop-signal task was used to evaluate indices of attention and response inhibition function, considered to be the hallmark of executive control. Correlations between task performance, ERPs and haemoglobin were also examined. Results showed impaired cognitive performance in ß-TM patients, as indicated by longer response times than controls. Haemoglobin was negatively correlated with response times to Go stimuli. Electrophysiological results indicated significant ß-TM-related alterations in neuronal activity, reflected in greater peak amplitudes of several task-related ERP components. A possible interpretation of these ERP results is that ß-TM patients need to recruit additional brain resources when dealing with cognitive challenge. Significant correlations were found between levels of haemoglobin and amplitude of all ERP components; the lower the haemoglobin, the more pronounced the ERPs amplitude. The present study represents a novel investigation of cognitive function and related brain dynamics in ß-TM in adult. Integrating neuropsychological assessment and interventions into traditional disease management, may be imperative in achieving a better quality of life for these patients.

Response Patterns to Emotional Faces Among Adolescents Diagnosed With ADHD.

OBJECTIVE: The present study investigated differences in emotional face processing between adolescents (age 15-18) with ADHD-Combined type (ADHD-CT) and typically developing controls. METHOD: Participants completed a visual emotional task in which they were asked to rate the degree of negativity/positivity of four facial expressions (taken from the NimStim face stimulus set). RESULTS: Participants' ratings, ratings' variability, response times (RTs), and RTs' variability were analyzed. Results showed a significant interaction between group and the type of presented stimuli. Adolescents with ADHD-CT discriminated less between positive and negative emotional expressions compared with those without ADHD. In addition, adolescents with ADHD-CT exhibited greater variability in their RTs and in their ratings of facial expressions when compared with controls. CONCLUSION: The present results lend further support to the existence of a specific deficit or alteration in the processing of emotional face stimuli among adolescents with ADHD-CT.

Executive function and neural activation in adults with ß-thalassemia major: an event-related potentials study.

In recent years, there has been growing interest in understanding the involvement of the nervous system and neurological complications in ß-thalassemia major (ß-TM). Several reports have demonstrated ß-TM-related neurological abnormalities, and these have been postulated to be responsible for impaired cognitive and neuropsychological functioning. We investigated neural correlates of cognitive function in adults with ß-TM and healthy controls using scalp-recorded event-related potentials (ERPs). To date, there have been no ERP studies in ß-TM adult patients. We identified ERP correlates of executive function by using a complex task-switching paradigm in which participants have to quickly and effectively switch between two different task sets. The results indicated poorer cognitive performance of ß-TM patients, resulting in overall higher error rates, longer response times, and increased switch costs compared with controls. Hemoglobin levels were negatively correlated with error rates and response times. Electrophysiological results indicated significant alterations in peak amplitudes of the ERP components P1, N1, and P2 in ß-TM patients relative to controls. P2 amplitude correlated with hemoglobin levels. This novel investigation of executive function and related brain mechanisms and dynamics in adults with ß-TM underscores the usefulness of ERP methodology as a sensitive measure for the study of neurocognitive processes in ß-TM.

Cognitive functions in adults with ß-thalassemia major: before and after blood transfusion and comparison with healthy controls.

While ß-thalassemia major (ß-TM)-related physiological complications have been well established, less is known about implications for neuropsychological and cognitive function. The few existing studies have focused almost exclusively on children. We evaluated cognitive function in adult ß-TM patients compared to healthy controls (study 1) and in ß-TM patients before and after blood transfusion (study 2). Performance intelligence quotient (IQ) was evaluated with four subtests from the Wechsler Adult Intelligence Scale (WAIS-III). Attention functions were evaluated using the online continuous performance test (OCPT). The results of study 1 revealed poorer performance of ß-TM patients on three of the four intelligence subtests, with significantly lower total performance IQ scores compared with controls. The percentage of participants with abnormal performance IQ (<85) was almost five times higher in the ß-TM group (58%) than in the control group (12%). In study 2, significant differences were found in OCPT performance as a function of blood transfusion. Before transfusion, patients had higher rates of omission and commission errors, slower response times (RTs), and lower RT consistency than after transfusion. As ß-TM patients' life expectancy is increasing, assessment and treatment of neurocognitive functions should become an integral part of appropriate follow-up to improve patients' quality of life.

Psychological and cortisol reactivity to experimentally induced stress in adults with ADHD.

Individuals with ADHD suffer from increased vulnerability to environmental and mental stressors and may be at increased risk for chronic stress in everyday life. The Hypothalamic-Pituitary-Adrenal (HPA) axis is a critical physiological system that mediates responses to stress. The present study seeks to examine test performance, test anxiety, self-reported psychological stress and cortisol reactivity to mental-cognitive stress in adults with ADHD when compared with healthy controls. Stress was induced by an arithmetic ability test. Psychological stress was assessed repeatedly throughout the experimental session. Salivary cortisol, an indicator of the HPA axis function, was evaluated immediately upon arrival, as well as 1 min and 20 min post-test completion. Results revealed higher levels of test anxiety and poorer performance on the test in the ADHD group. The ADHD and control groups showed no difference in base-line levels of subjective stress and in subjective stress levels 20 min after the test. In contrast, individuals with ADHD reported significantly higher levels of stress at the test anticipation phase and 1 min post-test completion. Cortisol response to stress differed according to group: in the ADHD group, 20 min post-test cortisol levels were significantly higher than base-line cortisol levels. This was not evident in the control group. These results suggest greater activation of the HPA axis in response to stress in adults with ADHD when compared with healthy controls. Adults with ADHD do not differ from controls in basal levels of subjective stress and cortisol, but do have stronger psychophysiological reactions in response to stressful challenges. The present findings are among the first to demonstrate significant alterations in cortisol reactivity to stress in adults with ADHD.

Behavioral and neural correlates of facial versus nonfacial stimuli processing in adults with ADHD: An ERP study.

OBJECTIVE: The present study was aimed at identifying behavioral and neural correlates of face processing in adults with attention-deficit/hyperactivity disorder (ADHD) using scalp-recorded event-related potentials (ERPs). METHOD: Behavioral and ERP responses were examined using a 4-stimulus visual oddball task, combining facial and nonfacial stimuli. Responses to target and nontarget stimuli were compared across groups of young adults with ADHD and comparison subjects. RESULTS: Participants with ADHD had poorer performance than controls on all indices of the oddball task. Higher rates of omissions and slower reaction times (RTs) of ADHD participants compared with controls had been found only in response to face targets but not in response to nonfacial targets. ERP results indicated that ADHD significantly interacted with the type of target stimuli. Participants with ADHD, compared with controls, showed a pronounced reduction in P3 and N3 amplitudes in response to face targets but not in response to nonfacial targets. CONCLUSIONS: The current results provide indication of modulation of face processing in adults with ADHD. ERP alterations, reflected in abnormally reduced P3 and N3 to face targets, may suggest ADHD-related abnormal recruitment of neural resources to process face stimuli. Behavioral and brain function measures of face processing may provide valuable additional tools for use in clinical assessment of ADHD in adulthood.

The relationships among ADHD, self-esteem, and test anxiety in young adults.

OBJECTIVE: The comorbidity of adult ADHD with test anxiety (TA) has not been previously reported. This comorbidity can potentially affect clinical and academic interventions among individuals with ADHD. The present study investigated the relationships among ADHD, self-esteem, and three subscales of TA among young adults: Cognitive Obstruction, Social Derogation, and Tenseness. METHOD: A total of 25 female participants diagnosed with ADHD and 30 female controls without ADHD of comparable age and education completed an Online Continuous Performance Test, an ADHD questionnaire, a self-esteem inventory, and a TA questionnaire. RESULTS: Participants with ADHD exhibited significantly higher levels of TA on all three subscales and lower levels of self-esteem compared with controls. Self-esteem served as a partial mediator between ADHD and cognitive obstruction TA and as a full mediator between ADHD and social derogation TA, but had no mediation effect in the relationships between ADHD and tenseness TA. CONCLUSION: The findings of this study suggest that TA, well known to affect success on tests, is correlated with ADHD. Therefore, interventions for ADHD should include components aimed at reducing TA.

Altered event-related potentials in adults with ADHD during emotional faces processing.

OBJECTIVE: This study investigated behavioral and neural correlates of emotional processing in adults with ADHD using scalp-recorded event-related potentials (ERPs). METHODS: We used a visual-emotional oddball paradigm, in which subjects were confronted with neutral and emotional faces (happy and angry). Responses to target and non-target stimuli were compared across groups of 17 adults with ADHD and 20 control subjects. RESULTS: Participants with ADHD had slower RTs than controls in response to happy but not to angry faces. ADHD participants, but not controls, responded faster to angry than to happy faces. ERP results indicated that group significantly interacted with the type of facial expression. P1 was significantly increased for the ADHD group compared with controls, but only to emotional (and not to neutral) faces. In the ADHD group, but not in controls, P1 was greater in response to emotional compared with neutral faces. N170 was more pronounced to angry than to happy faces in the ADHD group, while in the control group N170 was more pronounced to happy than to angry faces. Participants with ADHD showed a pronounced reduction in P3 to both emotional and neutral faces. CONCLUSIONS: The current results provide indication of altered behavioral responses as well as altered P1, N170 and P3 to emotional faces in adults with ADHD compared with healthy controls. SIGNIFICANCE: Behavioral and brain function measures of emotion processing may provide valuable additional tools for clinical assessment of ADHD in adulthood.

Neural correlates of emotional intelligence in a visual emotional oddball task: an ERP study.

The present study was aimed at identifying potential behavioral and neural correlates of Emotional Intelligence (EI) by using scalp-recorded Event-Related Potentials (ERPs). EI levels were defined according to both self-report questionnaire and a performance-based ability test. We identified ERP correlates of emotional processing by using a visual-emotional oddball paradigm, in which subjects were confronted with one frequent standard stimulus (a neutral face) and two deviant stimuli (a happy and an angry face). The effects of these faces were then compared across groups with low and high EI levels. The ERP results indicate that participants with high EI exhibited significantly greater mean amplitudes of the P1, P2, N2, and P3 ERP components in response to emotional and neutral faces, at frontal, posterior-parietal and occipital scalp locations. P1, P2 and N2 are considered indexes of attention-related processes and have been associated with early attention to emotional stimuli. The later P3 component has been thought to reflect more elaborative, top-down, emotional information processing including emotional evaluation and memory encoding and formation. These results may suggest greater recruitment of resources to process all emotional and non-emotional faces at early and late processing stages among individuals with higher EI. The present study underscores the usefulness of ERP methodology as a sensitive measure for the study of emotional stimuli processing in the research field of EI.

Reliability, validity and sensitivity of a computerized visual analog scale measuring state anxiety.

BACKGROUND AND OBJECTIVES: Assessment of state anxiety is frequently required in clinical and research settings, but its measurement using standard multi-item inventories entails practical challenges. Such inventories are increasingly complemented by paper-and-pencil, single-item visual analog scales measuring state anxiety (VAS-A), which allow rapid assessment of current anxiety states. Computerized versions of VAS-A offer additional advantages, including facilitated and accurate data collection and analysis, and applicability to computer-based protocols. Here, we establish the psychometric properties of a computerized VAS-A. METHODS: Experiment 1 assessed the reliability, convergent validity, and discriminant validity of the computerized VAS-A in a non-selected sample. Experiment 2 assessed its sensitivity to increase in state anxiety following social stress induction, in participants with high levels of social anxiety. RESULTS: Experiment 1 demonstrated the computerized VAS-A's test-retest reliability (r = .44, p < .001); convergent validity with the State-Trait Anxiety Inventory's state subscale (STAI-State; r = .60, p < .001); and discriminant validity as indicated by significantly lower correlations between VAS-A and different psychological measures relative to the correlation between VAS-A and STAI-State. Experiment 2 demonstrated the VAS-A's sensitivity to changes in state anxiety via a significant pre- to during-stressor rise in VAS-A scores (F(1,48) = 25.13, p < .001). LIMITATIONS: Set-order administration of measures, absence of clinically-anxious population, and gender-unbalanced samples. CONCLUSIONS: The adequate psychometric characteristics, combined with simple and rapid administration, make the computerized VAS-A a valuable self-rating tool for state anxiety. It may prove particularly useful for clinical and research settings where multi-item inventories are less applicable, including computer-based treatment and assessment protocols. The VAS-A is freely available: http://people.socsci.tau.ac.il/mu/anxietytrauma/visual-analog-scale/.

Reliability and validity of the Online Continuous Performance Test among children.

OBJECTIVE: The study assesses the reliability and validity of a new Online Continuous Performance Test (OCPT) for measuring sustained attention, response inhibition, and response time consistency among children. METHOD: The study sample comprised 73 children (6-13 years), 47 children with attention deficit hyperactivity disorder and 24 in the control group. The Diagnostic Interview Schedule for Children was administered to participants' parents to confirm group allocation. Children completed the OCPT in a laboratory setting, and a week later completed the OCPT at home. RESULTS: Split-half correlation coefficients reflected high levels of reliability in the laboratory and at home. Significant correlations were found between the laboratory- and home-based OCPT scores. Significant differences in OCPT performance were found between children with and without attention deficit hyperactivity disorder on the OCPT in the two settings. CONCLUSIONS: These results support the reliability and validity of the OCPT and suggest that it may serve as an effective tool for the assessment of children's attention function in naturalistic settings.

Behavioral and neural correlates of cognitive-affective function during late pregnancy: an Event-Related Potentials study.

The present study was aimed at identifying potential behavioral and neural correlates of cognitive and emotional processing during pregnancy using scalp-recorded Event-Related Potentials (ERPs). We used a 4-stimulus visual oddball task, combining emotional and non-emotional stimuli. Responses to target and non-target stimuli were compared across groups of 17 pregnant women on their third trimester and 19 non-pregnant women. Participants also completed a non-emotional test of sustained attention and response inhibition; the Online Continuous Performance Test (OCPT). Pregnant women had poorer performance than controls on most indices of the OCPT and the oddball task. ERP results indicated that pregnancy significantly interacted with the type of target stimuli. Results of the P3 component have demonstrated a comparative reduction in P3 amplitude in pregnant women in response to the target emotional faces but not in response to the target shapes. Moreover, among pregnant women, P3 amplitude was greater for the target shapes than for the target faces, while in non-pregnant women P3 amplitude was greater for the target faces than for the target shapes. Results of the N170 component showed that N170 to faces, but not to shapes, was more pronounced in pregnant women compared with non-pregnant women. The current results provide indication of modulation of cognitive-affective function during pregnancy. ERP alterations may suggest changes in the recruitment of neural resources to process emotional stimuli and alterations in attention allocation and evaluation of emotional stimuli among pregnant women.

Reliability and validity of the online continuous performance test among young adults.

Continuous Performance Tests (CPTs) are used in research and clinical contexts to measure sustained attention and response inhibition. Reliability and validity of a new Online Continuous Performance Test (OCPT) was assessed. The OCPT is designed for delivery over the Internet, thereby opening new opportunities for research and clinical application in naturalistic settings. In Study 1, participants completed the OCPT twice over a 1-week period. One test was taken at home and one in the laboratory. Construct validity was assessed against a gold standard CPT measure. Results indicate acceptable reliability between the home- and laboratory-administered tests. Modest to high correlations were observed between the OCPT scales and the corresponding scales of the gold standard CPT. Study 2 examined whether the OCPT may discriminate participants with attention deficit hyperactivity disorder from healthy controls. Results revealed significantly higher rates of omission and commission errors and greater response time variability in participants with attention deficit hyperactivity disorder relative to healthy controls. These results support the reliability and validity of the OCPT and suggest that it may serve as an effective tool for the assessment of attention function in naturalistic settings.

Behavioral and neural correlates of emotional intelligence: an event-related potentials (ERP) study.

The present study was aimed at identifying potential behavioral and neural correlates of emotional intelligence (EI) by using scalp-recorded Event-Related Potentials (ERPs). EI levels were defined according to both self-report questionnaire and a performance-based test. We identified ERP correlates of emotional processing by comparing ERPs elicited in trials using pleasant, neutral and unpleasant pictures. The effects of these emotion-inducing pictures were then compared across groups with low and high EI levels. Behavioral results revealed a significant valence×EI group interaction effect since valence ratings were lower for unpleasant pictures and higher for pleasant pictures in the high EI group compared with the low EI group. The groups did not differ with respect to neutral picture ratings. The ERP results indicate that participants with high EI exhibited significantly greater mean amplitudes of the P2 (200-300ms post-stimulus) and P3 (310-450ms post-stimulus) ERP components in response to emotional and neutral pictures, at posterior-parietal as well as at frontal scalp locations. This may suggest greater recruitment of resources to process all emotional and non-emotional stimuli at early and late processing stages among individuals with higher EI. The present study also underscores the usefulness of ERP methodology as a sensitive measure for the study of emotional stimuli processing in the research field of EI.

Ameliorative effects of brief daily periods of social interaction on isolation-induced behavioral and hormonal alterations.

The present study investigated the effects of brief daily periods of social interaction on social-isolation-induced behavioral and hormonal alterations and deficits. Adult male Wistar rats were allocated to one of three housing conditions: 1) social housing (two per cage); 2) social isolation (one per cage); or partial social isolation (one per cage with access to another male rat for 60 min/day). After 14 days in these different housing conditions, the animals were subjected to various behavioral tests, including sucrose preference test, acoustic startle response, two-way active shuttle avoidance, pre-pulse inhibition, open field, cooperation learning task, and levels of corticosterone. Results revealed that social isolation had a substantial impact on rats' performance on most behavioral tests as well as on their corticosterone levels. Importantly, however, the results clearly demonstrate that allowing otherwise isolated animals to have a brief (60 min) daily social contact with another rat to a great extent abolishes or ameliorates most of the isolation-induced behavioral and hormonal alterations. Hence, providing isolated animals with brief daily periods of social contact may be used as a "preventive treatment" in order to protect them from the deleterious effects of isolation.