Subject(s)
Catecholamines/physiology , Chickens/physiology , Eggs , Animals , Brain Chemistry , Egg Yolk/analysis , Female , Ovalbumin/analysis , OvulationABSTRACT
Two experiments were conducted on White Leghorn hens to investigate the effect of different concentrations of LH on the frequency of induction of ovulation in vitro of ovarian follicles collected 18-20 h before the expected time of ovulation from birds of three different ages (7, 18 and 30 months) or of the same age but laying short (three to four) or long (more than seven) sequences of eggs. The ability of LH to induce ovulation was directly related to the age of the donors of the follicles. Follicles derived from hens laying short sequences of eggs were less responsive to the ovulation-inducing effects of LH than were follicles from birds laying long sequences. These observations suggest that the sensitivity of ovarian follicles to the ovulation-inducing effects of LH declines with age and is greater in hens laying long sequences of eggs than those laying short sequences.
Subject(s)
Luteinizing Hormone/pharmacology , Oviposition , Ovulation Induction , Age Factors , Animals , Chickens , Female , In Vitro Techniques , Ovarian Follicle/drug effectsSubject(s)
Chickens/physiology , Ovarian Follicle/physiology , Oviposition , Ovulation , Animals , Culture Media , Epinephrine/pharmacology , Female , Imidazoles/pharmacology , In Vitro Techniques , Luteinizing Hormone/pharmacology , Ovarian Follicle/drug effects , Ovulation/drug effects , Tolazoline/pharmacologyABSTRACT
Spermatozoal and seminal plasma concentrations of total lipids from 50 ejaculates and phospholipids and their fractions from 30 ejaculates were quantified in the semen of five Murrah buffalo bulls. Sperm lipid content ranged from 0.93 to 1.72 mg/10(9) cells with an overall average 1.32+/-0.03 mg/10(9) cells. Its concentration in seminal plasma varied from 1.39 to 2.22 mg/ml with overall average of 1.75+/-0.03 mg/ml. Spermatozoal total phospholipid content ranged from 0.44 to 0.94 mg/10(9) cells with overall mean being 0.64+/-0.02 mg/10(9) cells. The corresponding values for seminal plasma were 0.53 and 0.88 mg/ml with an overall mean of 0.69+/-0.02 mg/ml. Phosphatidyl choline constituted the major fraction both in the spermatozoa and and seminal plasma.
ABSTRACT
Dopamine (DA) concentrations in the largest ovarian follicle (F1) showed significant decrease at 12 and 8 h prior to ovulation. Nor-epinephrine (NE) and epinephrine (E) concentrations in the F1, sampled at different times of the ovulatory cycle, were found to increase with the approach of ovulation time. However, the concentration of epinephrine in the F1 was low just after oviposition compared with the immediate two previous stages investigated. The concentrations of DA, NE and E in the second largest follicles (F2) did not vary significantly during the ovulatory cycle. Average concentration of DA was low and that of NE and E high in the F1 compared with the respective concentrations in the F2.
Subject(s)
Catecholamines/metabolism , Chickens/physiology , Ovarian Follicle/metabolism , Ovulation , Animals , Dopamine/metabolism , Epinephrine/metabolism , Female , Norepinephrine/metabolism , OvipositionABSTRACT
Prolactin levels were quantified by double antibody radio-immunoassay in the blood sampled daily for 24 days, from 9 non-cyclic Murrah buffaloes during hot months (ambient temp.41 to 43 degrees C). All buffaloes were kept in open loose housing. Three buffaloes were sprinkled with water for half an hour twice daily between 1200 and 1430 h to overcome partly the effect of heat during the period of investigation. Mean prolactin levels of 6 unsprinkled buffaloes ranged on different days from 249 to 739 ng/ml serum. The range of averages in case of 3 sprinkled animals was from 152 to 342 ng/ml serum. Buffaloes subjected to sprinkling had significantly (P / 0.01) lower prolactin levels than control buffalo.
ABSTRACT
Circulating FSH levels of 13 Murrah buffaloes were investigated by a double-antibody radioimmunoassay using a homologous bovine system. The mean (+/- s.e.m.) peak FSH level on the day of estrus was if57.94 +/- 12.84 ng/ml of blood serum during hotter months (June-August) and 65.23+/-10.54 ng/ml during cooler months (October-December). These were much higher than the FSH concentrations recorded during the rest of the cycle post-mating. There were sizable fluctuations in FSH levels on different days of the estrous cycle within animals. However, the mean values did not exhibit much variation. The occurrence of the FSH peak and the LH peak coincided during the cycle.
ABSTRACT
The concentration of luteinizing hormone (LH) was measured in the serum of pre- and post-pubertal Murrah buffaloes, in addition to during pregnancy and lactation. The mean values varied between 0.6 +/- 0.11 (S.E.) and 1.4 +/- 0.18 ng/ml, but the differences observed were not significant (P>0.05).
Subject(s)
Catecholamines/pharmacology , Chickens/physiology , Luteinizing Hormone/pharmacology , Ovulation/drug effects , Receptors, Adrenergic, alpha/drug effects , Receptors, Adrenergic/drug effects , Animals , Dopamine/pharmacology , Epinephrine/pharmacology , Female , Norepinephrine/pharmacologySubject(s)
Prolactin/immunology , Animals , Cattle , Immune Sera , Rabbits/immunology , Radioimmunoassay/methodsABSTRACT
Circulating LH levels of 14 Murrah buffaloes were determined by a double-antibody radioimmunoassay using an homologous bovine system. At the onset of oestrus, the mean peak circulating LH level was 20.80 +/- 3.43 (s.e.m.) ng/ml in the hotter months (June-August) and 21.24 +/- 0.98 ng/ml during the cooler months (October-December), values remaining high for about 4 h. Basal levels throughout the rest of the cycle averaged 1-2 ng/ml. Conception occurred in 7 of the animals showing LH peaks (2/8 in June-August, 5/6 in October-December).
