Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 7 de 7
Filter
1.
J Clin Periodontol ; 27(9): 698-704, 2000 Sep.
Article in English | MEDLINE | ID: mdl-10983604

ABSTRACT

BACKGROUND: Transforming growth factor-beta (TGF-beta) represents a family of growth-modulating proteins with fundamental roles in connective tissue and bone development. The objective of this study was to evaluate the potential for regeneration of alveolar bone and cementum following surgical implantation of recombinant human TGF-beta 1 (rhTGF-beta 1). METHOD: Bilateral, critical size, supra-alveolar periodontal defects in 5 beagle dogs were surgically implanted with rhTGF-beta 1 in a calcium carbonate carrier (CaCO3) or with carrier alone. The animals were euthanized at 4 weeks postsurgery and block-biopsies of the defects were processed for histologic and histometric analysis. RESULTS: Surgical implantation of rhTGF-beta 1 resulted in minimal, if any, stimulation of alveolar bone or cementum regeneration. Linear bone and cementum regeneration in rhTGF-beta 1-treated defects was 1.2+/-0.6 and 0.01+0.01 mm, respectively. Corresponding values for the controls were 1.0+/-0.6 and 0.01+/-0.03 mm. CONCLUSIONS: The results suggest that, under the conditions (dose, carrier, defect type) evaluated here, treatment of periodontal defects in beagle dogs with rhTGF-beta 1 may be of limited clinical benefit.


Subject(s)
Alveolar Process/drug effects , Bone Regeneration/drug effects , Dental Cementum/drug effects , Periodontal Diseases/drug therapy , Transforming Growth Factor beta/administration & dosage , Alveolar Process/pathology , Alveolar Process/physiopathology , Animals , Bone Regeneration/physiology , Dental Cementum/pathology , Dental Cementum/physiopathology , Disease Models, Animal , Dogs , Drug Evaluation, Preclinical , Drug Implants , Humans , Male , Periodontal Diseases/pathology , Periodontal Diseases/physiopathology , Recombinant Proteins/administration & dosage
3.
J Clin Periodontol ; 25(6): 475-81, 1998 Jun.
Article in English | MEDLINE | ID: mdl-9667481

ABSTRACT

This study evaluated alveolar bone and cementum regeneration following surgical implantation of recombinant human transforming growth factor-beta1 (rhTGF-beta1) in conjunction with guided tissue regeneration (GTR). Supraalveolar, critical size, periodontal defects were surgically created around the 3rd and 4th mandibular premolar teeth in right and left jaw quadrants in 5 beagle dogs. Alternate jaw quadrants in consecutive animals received rhTGF-beta1, in a CaCO3/hydroxyethyl starch carrier with GTR, or carrier with GTR alone (control). 20 microg of rhTGF-beta1 in buffer solution was incorporated into approximately 0.8 ml of carrier for each defect scheduled to receive rhTGF-beta1. Animals were sacrificed at week 4 postsurgery and tissue blocks were harvested and processed for histometric analysis. Clinical healing was generally uneventful. Minor membrane exposures were observed. Defects with membrane exposure displayed an inflammatory infiltrate underneath the membrane. Bone regeneration of trabecular nature, apparent in all animals, was generally limited to the very apical aspect of the defects. Cementum regeneration was limited without obvious differences between experimental conditions. Comparing rhTGF-beta1, to control defects, statistically significant differences were found for area (1.8+/-0.4 and 1.3+/-0.6 mm2, respectively; p<0.05) and density (0.3+/-0.1 and 0.2+/-0.03, respectively; p<0.05) of alveolar bone regeneration. Observed differences are small and represent a clinically insignificant potential for enhanced regeneration in this preclinical model. Within the limitations of study, it may be concluded that rhTGF-beta1 has a restricted potential to enhance alveolar bone regeneration in conjunction with GTR.


Subject(s)
Guided Tissue Regeneration, Periodontal/methods , Transforming Growth Factor beta/therapeutic use , Alveolar Bone Loss/surgery , Alveolar Process/pathology , Animals , Bicuspid , Bone Regeneration , Calcium Carbonate , Dental Cementum/pathology , Dogs , Drug Carriers , Furcation Defects/surgery , Humans , Hydroxyethyl Starch Derivatives , Membranes, Artificial , Periodontal Diseases/surgery , Polytetrafluoroethylene , Recombinant Proteins , Regeneration , Transforming Growth Factor beta/administration & dosage
4.
J Urol ; 158(2): 519-21, 1997 Aug.
Article in English | MEDLINE | ID: mdl-9224337

ABSTRACT

PURPOSE: We attempted to determine whether an introducer tip catheter reduces urinary tract infection in spinal cord injured patients on intermittent catheterization. MATERIALS AND METHODS: The introducer tip catheter bypasses the colonized 1.5 cm. of the distal urethra. Enrolled patients were prospectively entered into the study in alternate groups depending on whether they reflex voided: group 1--on intermittent catheterization with the introducer tip catheter but not voiding spontaneously or wearing an external urinary catheter, group 2--same as group 1 but using a nonintroducer tip catheter; group 3--on intermittent catheterization with the introducer tip catheter, voiding by reflex and wearing an external urinary catheter, and group 4--same as group 3 but using a nonintroducer tip catheter. RESULTS: Statistical significance was shown when comparing patients using versus not using the introducer tip catheter regardless of whether an external urinary catheter was worn (p = 0.0121). A greater difference was noted between patients using and not using the introducer tip catheter in the intermittent catheterization only group (p = 0.0093). CONCLUSIONS: The introducer tip catheter decreased urinary tract infections in hospitalized men with spinal cord injury on intermittent catheterization.


Subject(s)
Spinal Cord Injuries/complications , Urinary Catheterization/adverse effects , Urinary Catheterization/instrumentation , Urinary Tract Infections/etiology , Urinary Tract Infections/prevention & control , Adolescent , Adult , Equipment Design , Humans , Incidence , Prospective Studies , Urinary Bladder/microbiology , Urinary Tract Infections/epidemiology
5.
J Spinal Cord Med ; 19(3): 197-200, 1996 Jul.
Article in English | MEDLINE | ID: mdl-8819030

ABSTRACT

Lower urinary tract reconstruction has been performed on the spinal injured population at our institution since 1988. Careful pre-operative evaluation including a detailed history, physical examination and radiographic and/or urodynamic studies are usually obtained to determine which type of procedure would be most beneficial for each individual patient. Typically, patients receive either a cutaneous (i.e., Kock or Indiana) diversion or undergo a form of bladder augmentation (usually ileocystoplasty). Pre-operative findings and the patient's history are carefully considered prior to choosing the appropriate procedure. Occasionally, a particular finding influences the decision. We describe a patient who received a hemi-Kock ileocystoplasty with a continent abdominal stoma who, in retrospect, would have benefitted from a supravesical diversion.


Subject(s)
Spinal Cord Injuries/surgery , Urinary Bladder, Neurogenic/surgery , Urinary Diversion/methods , Urinary Reservoirs, Continent/methods , Adult , Fournier Gangrene/surgery , Humans , Male , Postoperative Complications/surgery , Reoperation , Retrospective Studies , Suture Techniques , Treatment Outcome , Urodynamics/physiology
6.
Urology ; 46(6): 801-3, 1995 Dec.
Article in English | MEDLINE | ID: mdl-7502419

ABSTRACT

OBJECTIVES: Since 1986, we have offered the option of lower urinary tract reconstruction with the Kock ileal-urethral reservoir in selected male patients requiring diversion. This study provides insight into the functional characteristics of the Kock ileal-urethral reservoir and its effect on continence. METHODS: Twenty-four of the initial 225 male patients undergoing this procedure at our institution were evaluated by fluorourodynamics within 2 years of neobladder construction. Information regarding continence was also obtained by means of a patient interview and questionnaire. RESULTS: The average resting neobladder pressure was 8.5 cm H2O (range, 0 to 18). Reservoir capacity averaged 741 cc (range, 225 to 1400). Afferent nipple failure with bilateral grade II vesicoureteral reflux was noted in 1 patient (4%). Unsatisfactory daytime continence was seen in 2 patients (8%). Unsatisfactory nighttime continence was seen in 6 patients (25%). Patient satisfaction was high with an average rating of 8.6 on a scale of 1 to 10. CONCLUSIONS: Fluorourdynamic data demonstrate a low-pressure, high-capacity reservoir with a low incidence of reflux. The rate of continence is acceptable and patient satisfaction is excellent. The Kock ilealurethral reservoir is an excellent alternative to standard diversion for the male patient undergoing cystectomy.


Subject(s)
Urinary Reservoirs, Continent , Urodynamics , Adult , Aged , Aged, 80 and over , Fluoroscopy , Humans , Male , Middle Aged , Pressure , Treatment Outcome , Urinary Reservoirs, Continent/adverse effects , Urination , Vesico-Ureteral Reflux/etiology , Video Recording
7.
Funct Dev Morphol ; 1(2): 53-4, 1991.
Article in English | MEDLINE | ID: mdl-1790342

ABSTRACT

The corpuscles of Stannius of Botia lohachata are made up of numerous large lobules, each of which is bounded by a septum and contains two types of cells. The secretory granules are PAS- and AF-positive, indicating that 1,2 glycol groups and non-sulphated mucoid substances are present. Detached cells in the lobule lumen are suggestive of holocrine secretion. The possibility of the existence of more than one secretory product is discussed.


Subject(s)
Cypriniformes/anatomy & histology , Kidney/anatomy & histology , Animals
SELECTION OF CITATIONS
SEARCH DETAIL
...