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1.
Ulster Med J ; 85(3): 182-186, 2016 Sep.
Article in English | MEDLINE | ID: mdl-27698521

ABSTRACT

BACKGROUND: The Regulation and Quality Improvement Authority (RQIA) monitors the administration of electroconvulsive therapy (ECT) in Northern Ireland (NI). As part of their inspection methodology RQIA wished to include feedback from ECT patients. The aim of this report is to summarise the opinions of ECT patients over a 1-year period and to compare their feedback about treatment with the standards of best practice, as defined by the Electroconvulsive Therapy Accreditation Service (ECTAS). METHOD: RQIA was granted permission to use the ECTAS patient questionnaire. The questionnaire was distributed to all the ECT clinics in NI and staff were requested to give them to patients who had received a course of ECT. RESULTS: A total of 42 individuals returned questionnaires, 24 females (57.1%) and 18 (42.9%) males. The response rate was 26%. Almost half of respondents were detained under the Mental Health (Northern Ireland) Order 1986 (n=19, 45.2%), with one third receiving ECT as a day patient (n=14, 33.3%). Respondents reported having detailed information about ECT, with ECTAS standards 4.2 and 4.3 being affirmed in over 80% of cases. Eighty percent of respondents (n=34) believed they benefited from ECT. CONCLUSION: The results are mainly favourable towards ECT. The majority felt they benefited from treatment.


Subject(s)
Electroconvulsive Therapy/methods , Epilepsy/therapy , Patient Satisfaction , Quality Improvement , Surveys and Questionnaires , Adult , Aged , Female , Humans , Male , Middle Aged , Treatment Outcome
2.
J Anim Sci ; 92(12): 5757-61, 2014 Dec.
Article in English | MEDLINE | ID: mdl-25414113

ABSTRACT

Binding of IgG antibodies to Entodinium spp. in the rumen of sheep (Ovis aries) was investigated by adding IgG, purified from plasma, directly into the rumen. Plasma IgG was sourced from sheep that had or had not been immunized with a vaccine containing whole fixed Entodinium spp. cells. Ruminal fluid was sampled approximately 2 h after each antibody dosing. Binding of protozoa by a specific antibody was detected using an indirect fluorescent antibody test. An antibody titer in the ruminal fluid was determined by ELISA, and the concentration of ruminal fluid ammonia-N and ruminal pH were also determined. Entodinium spp. and total protozoa from IgG-infused sheep were enumerated by microscopic counts. Two-hourly additions of IgG maintained a low antibody titer in the rumen for 12 h and the binding of the antibody to the rumen protozoa was demonstrated. Increased ammonia-N concentrations and altered ruminal fluid pH patterns indicated that additional fermentation of protein was occurring in the rumen after addition of IgG. No reduction in numbers of Entodinium spp. was observed (P>0.05). Although binding of antibodies to protozoa has been demonstrated in the rumen, it is unclear how much cell death occurred. On the balance of probability, it would appear that the antibody was degraded or partially degraded, and the impact of this on protozoal populations and the measurement of a specific titer is also unclear.


Subject(s)
Antibodies, Protozoan/immunology , Ciliophora/immunology , Ciliophora/isolation & purification , Rumen/parasitology , Sheep/immunology , Ammonia/metabolism , Animals , Antibodies, Protozoan/blood , Fermentation , Hydrogen-Ion Concentration , Immunoglobulin G/blood , Immunoglobulin G/immunology , Male , Protozoan Vaccines/immunology , Sheep/blood , Time Factors
3.
Burns ; 38(1): 32-43, 2012 Feb.
Article in English | MEDLINE | ID: mdl-22079536

ABSTRACT

Burns can result in long term impairments, activity limitations and participation restrictions in a patients' life. The focus of current surgeries and therapy is to improve body functions and structures. However, often this does not translate to an improvement in activity and participation for the patient. Improvement in activity and participation is the ultimate goal of all therapy to enhance patient's quality of life. The incorporation of assessment measures at all levels of the International Classification of Functioning, Disability and Health (ICF) can assist in a holistic, patient centred approach to identify the complex impairments that impact on activity and participation, with a view to appropriately targeting future therapeutic interventions. This paper presents an example case of how implementing measures at all levels of the ICF can improve our understanding of a patient's body functions and structures, activity and participation. A number of the outcome measures utilised in this study are novel in the burns population, such that video footage supplements the methodology where relevant.


Subject(s)
Burns/classification , Disability Evaluation , International Classification of Diseases , Activities of Daily Living , Adult , Burns/physiopathology , Burns/rehabilitation , Child , Female , Humans , Male , Range of Motion, Articular , Surveys and Questionnaires
4.
Vaccine ; 22(29-30): 3976-85, 2004 Sep 28.
Article in English | MEDLINE | ID: mdl-15364447

ABSTRACT

This work was conducted to determine if methane emissions from sheep immunized with an anti-methanogen vaccine were significantly lower than methane emissions from non-immunized sheep, to test the effectiveness of two different vaccine formulations (VF) on methane abatement, and to compare methane emissions measured using a closed-circuit respiration chamber and the sulphur-hexafluoride (SF6) tracer technique. Thirty mature wether sheep were randomly allocated to three treatment groups (n = 10). One group received an immunization of adjuvant only on days 0 and 153 (control), a second group received an immunization with a 3-methanogen mix on days 0 and 153 (VF3 + 3), and a third group received an immunization of a 7-methanogen mix on day 0 followed by a 3-methanogen mix on day 153 (VF7 + 3). Four weeks post-secondary immunization, there was a significant 7.7% reduction in methane production per kg dry matter intake in the VF7 + 3 group compared to the controls (P = 0.051). However, methane emissions from sheep immunized with VF7 + 3 were not significantly different when compared to the sheep in the control group (P = 0.883). The average IgG and IgA antibody titres in both plasma and saliva of the VF3 + 3 immunized sheep were four to nine times higher than those immunized with VF7 + 3 (P< 0.001) at both 3 and 6 weeks post-secondary immunization. Data also revealed that SF6 methane estimates were consistently higher than the respiration chamber estimates and that there was no significant correlation between the SF6 methane estimates and the respiration chamber methane estimates (R2 = 0.11).


Subject(s)
Archaea/immunology , Archaea/metabolism , Methane/metabolism , Rumen/microbiology , Sheep/microbiology , Vaccines/immunology , Adjuvants, Immunologic , Animals , Antibodies, Archaeal/analysis , Immunization, Secondary , Immunoglobulin A/analysis , Immunoglobulin G/analysis , Kinetics , Methanobacterium/immunology , Methanobacterium/metabolism , Methanobrevibacter/immunology , Methanobrevibacter/metabolism , Methanomicrobiaceae/immunology , Methanomicrobiaceae/metabolism , Methanosarcina/immunology , Methanosarcina/metabolism , Rumen/immunology , Saliva/immunology , Sheep/immunology , Time Factors , Vaccines/administration & dosage
5.
J Mater Sci Mater Med ; 15(9): 997-1005, 2004 Sep.
Article in English | MEDLINE | ID: mdl-15448407

ABSTRACT

HAPEX trade mark (40 vol % hydroxyapatite in a high-density polyethylene matrix) and AWPEX (40 vol % glass-ceramic apatite-wollastonite in a high-density polyethylene matrix) are composites designed to provide bioactivity and to match the mechanical properties of human cortical bone. HAPEX trade mark has had clinical success in middle ear and orbital implants, and there is great potential for further orthopaedic applications of these materials. However, more detailed in vitro investigations must be performed to better understand the biological interactions of the composites. In this study, the bioactivity of each material was assessed. Specifically, the effects of controlled surface topography and ceramic filler composition on apatite layer formation in acellular simulated body fluid (SBF) with ion concentration similar to those of human blood plasma were examined. Samples were prepared as 1 x 10 x 10 mm(3) tiles with polished, roughened or parallel-grooved surface finishes, and were incubated in 20 ml of SBF at 36.5 degrees C for one, three, seven or 14 days. The formation of an apatite layer on the composite surface after immersion was demonstrated by thin-film X-ray diffraction, environmental scanning electron microscopy and energy dispersive X-ray analysis. Variations in sample weight and solution pH over the period of incubation were also recorded. Significant differences were found between the two materials tested, with greater bioactivity in AWPEX than HAPEX trade mark. Results also showed surface topography to be important, with rougher samples correlated to earlier apatite formation. Osteoblast-like cells proliferated favourably on both composite materials, with many filopodia connections, preferential attachment to ceramic particles and contact guidance effects evident.


Subject(s)
Biocompatible Materials/chemistry , Ceramics/chemistry , Polymers/chemistry , Apatites , Blood/metabolism , Cell Line, Tumor , Cell Proliferation , Humans , Hydrogen-Ion Concentration , Ions , Microscopy, Electron, Scanning , Osteoblasts/cytology , Osteoblasts/metabolism , Polyethylene , Surface Properties , Time Factors , X-Ray Diffraction
6.
Biomaterials ; 25(18): 4503-12, 2004 Aug.
Article in English | MEDLINE | ID: mdl-15046941

ABSTRACT

Glass-ceramic apatite-wollastonite (A-W)/high-density polyethylene composite (AWPEX) materials have been designed to match the mechanical strength of human cortical bone and to provide favourable bioactivity, with potential use in many orthopaedic applications. To better understand AWPEX properties, the effects of surface finish and ceramic filler size and content on osteoblast-like cell attachment, proliferation, and differentiation were examined. Glass-ceramic content was tested at 30 and 50 vol% and median particle size at 4.5 and 7.7 microm. Samples were prepared as 1 x 10 x 10 mm(3) tiles with polished or rough surfaces, sterilized by gamma irradiation (2.5 Mrad), and characterized by scanning electron microscopy (SEM) and surface profilometry. Saos-2 human osteoblast-like cells were cultured on each surface at an initial concentration of 4500 cells/cm(2) for 1, 3, or 7 days. At each time point, adenosine triphosphate and alkaline phosphatase levels were measured to assess cell number and osteoblast differentiation. SEM imaging of cells on the composite surfaces showed preferential cell attachment to filler particles within the polymer matrix. Significant biochemical assay differences were found at 7 days, confirmed by ANOVA post-hoc testing using Bonferroni's correction. Overall, increased exposure of the glass-ceramic A-W phase in AWPEX through surface polishing, higher volume fraction and/or larger particle size was found to lead to an improved cell response.


Subject(s)
Apatites/chemistry , Bone Substitutes/chemistry , Ceramics/chemistry , Osteoblasts/cytology , Osteoblasts/physiology , Polyethylene/chemistry , Silicic Acid/chemistry , Cell Adhesion/physiology , Cell Differentiation/physiology , Cell Division/physiology , Cell Line , Humans , Manufactured Materials/analysis , Materials Testing , Surface Properties
7.
Scanning ; 25(4): 181-4, 2003.
Article in English | MEDLINE | ID: mdl-12926609

ABSTRACT

Human osteoblast-like (bone-forming) cells were imaged using environmental scanning electron microscopy (ESEM). The cells were hydrated, unfrozen, and uncoated. Specimens were cooled to 3 degrees C and imaged in water vapor, with partial pressures varying from saturated conditions to a humidity of approximately 50%, relative to pure water. The ESEM images show the presence of cell nuclei, nucleoli, and cytoplasmic membranes. Comparisons between chemically fixed and unfixed specimens (neither dried nor coated) show that cell morphologies are similar in both cases. These results are compared with a fixed, dried, carbon-coated specimen. Thermodynamic and kinetic arguments are used to show that humidities significantly lower than 100% correspond to metastable states suitable for stabilizing hydrated biological tissues and cells. The ability to perform observations with minimal specimen preparation is potentially useful for studying interactions between mammalian cells and biomaterials that are developed for tissue engineering. The methods employed are equally applicable to the study of specimens in the biological, materials, and physical sciences where careful control over specimen stability is required.


Subject(s)
Cytological Techniques/methods , Microscopy, Electron, Scanning/methods , Humans , Osteoblasts/cytology , Tumor Cells, Cultured
8.
FEMS Microbiol Lett ; 183(1): 81-8, 2000 Feb 01.
Article in English | MEDLINE | ID: mdl-10650206

ABSTRACT

16S rRNA clone library analysis was used to examine the biodiversity and community structure within the sediments of three hypersaline Antarctic lakes. Compared to sediment of low to moderate salinity Antarctic lakes the species richness of the hypersaline lake sediments was 2-20 times lower. The community of Deep Lake (32% salinity, average sediment temperature -15 degrees C) was made up almost entirely of halophilic Archaea. The sediment communities of two meromictic hypersaline lakes, Organic Lake (20% salinity, -7 degrees C) and Ekho Lake (15% salinity, 15 degrees C) were more complex, containing phylotypes clustering within the Proteobacteria and Cytophagales divisions and with algal chloroplasts. Many phylotypes of these lakes were related to taxa more adapted to marine-like salinity and perhaps derive from bacteria exported into the sediment from the lower salinity surface waters. The Ekho Lake clone library contained several major phylotypes related to the Haloanaerobiales, the growth of which appears to be promoted by the comparatively high in situ temperature of this lake.


Subject(s)
Archaea/isolation & purification , Bacteria/isolation & purification , Ecosystem , Fresh Water/microbiology , Geologic Sediments/microbiology , Antarctic Regions , Archaea/genetics , Bacteria/genetics , DNA, Archaeal/analysis , DNA, Archaeal/genetics , DNA, Bacterial/analysis , DNA, Bacterial/genetics , Gene Library , Genes, rRNA , Phylogeny , RNA, Ribosomal, 16S/genetics , Sodium Chloride , Water Microbiology
9.
Environ Microbiol ; 2(2): 227-37, 2000 Apr.
Article in English | MEDLINE | ID: mdl-11220308

ABSTRACT

16S rDNA clone library analysis was used to examine the biodiversity and community structure within anoxic sediments of several marine-type salinity meromictic lakes and a coastal marine basin located in the Vestfolds Hills area of Eastern Antarctica. From 69 to 130 (555 total) 16S rDNA clones were analysed from each sediment sample, and restriction fragment length polymorphism (RFLP) and sequence analysis grouped the clones into 202 distinct phylotypes (a clone group with sequence similarity of >0.98). A number of phylotypes and phylotype groups predominated in all libraries, with a group of 10 phylotypes (31% of clones) forming a novel deep branch within the low G+C Gram-positive division. Other abundant phylotypes detected in several different clone libraries grouped with Prochlorococcus cyanobacteria, diatom chloroplasts, delta proteobacteria (Desulfosarcina group, Syntrophus and Geobacterl Pelobacter/Desulphuromonas group), order Chlamydiales (Parachlamydiaceae) and Spirochaetales (wall-less Antarctic spirochaetes). Most archaeal clones detected (3.1% of clones) belonged to a highly diverged group of Euryarchaeota clustering with clones previously detected in rice soil, aquifer sediments and hydrothermal vent material. Little similarity existed between the phylotypes detected in this study and other clone libraries based on marine sediment, suggesting that an enormous prokaryotic diversity occurs within marine and marine-derived sediments.


Subject(s)
Marine Biology , Phylogeny , Water Microbiology , Antarctic Regions , Bacteroidetes/genetics , Cyanobacteria/genetics , DNA/analysis , DNA, Bacterial/analysis , DNA, Plant/analysis , DNA, Ribosomal/analysis , Deltaproteobacteria/genetics , Gram-Positive Bacteria/genetics , Molecular Sequence Data , Polymorphism, Restriction Fragment Length , RNA, Bacterial/analysis , RNA, Plant/analysis , RNA, Ribosomal, 16S/analysis , Sequence Analysis
10.
Int J Syst Bacteriol ; 47(4): 1040-7, 1997 Oct.
Article in English | MEDLINE | ID: mdl-9336903

ABSTRACT

A polyphasic taxonomic study was performed to characterize dissimilatory iron-reducing strains mostly isolated from Antarctic sea ice. The strains were isolated from samples of congelated (land-fast) sea ice, grease ice, and ice algal biomass collected from the coastal areas of the Vestfold Hills in eastern Antarctica (68 degrees S 78 degrees E). The strains were facultatively anaerobic, motile, and rod shaped, were capable of anaerobic growth either by fermentation of carbohydrates or by anaerobic respiration, and utilized a variety of electron acceptors, including nitrate, ferric compounds, and trimethylamine N-oxide. A phylogenetic analysis performed with 16S rRNA sequences showed that the isolates formed two groups representing novel lineages in the genus Shewanella. The first novel group included seawater-requiring, psychrophilic, chitinolytic strains which had DNA G + C contents of 48 mol%. The members of the second strain group were psychrotrophic and did not require seawater but could tolerate up to 9% NaCl. The strains of this group were also unable to degrade polysaccharides but could utilize a number of monosaccharides and disaccharides and had G + C contents of 40 to 43 mol%. The whole-cell-derived fatty acid profiles of the sea ice isolates were found to be similar to the profiles obtained for other Shewanella species. The omega-3 polyunsaturated fatty acid eicosapentaenoic acid (EPA) (20:5 omega 3) was detected in all of the sea ice isolates at levels ranging from 2 to 16% of the total fatty acids. EPA was also found at high levels in Shewanella hanedai (19 to 22%) and Shewanella benthica (16 to 18%) but was absent in Shewanella alga and Shewanella putrefaciens. On the basis of polyphasic taxonomic data, the Antarctic iron-reducing strains are placed in two new species, Shewanella frigidimarina sp. nov. (type strain, ACAM 591) and Shewanella gelidimarina sp. nov. (type strain, ACAM 456).


Subject(s)
DNA, Bacterial/analysis , Gram-Negative Facultatively Anaerobic Rods/classification , Gram-Negative Facultatively Anaerobic Rods/genetics , Phylogeny , RNA, Ribosomal, 16S/analysis , Antarctic Regions , Base Composition , Eicosapentaenoic Acid/biosynthesis , Fatty Acids/analysis , Gram-Negative Facultatively Anaerobic Rods/chemistry , Gram-Negative Facultatively Anaerobic Rods/metabolism , Iron/chemistry , Molecular Sequence Data , Nucleic Acid Hybridization , Phenotype , Water Microbiology
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