The EU Interreg Project "GEREMIA" on waste management for the improvement of port waters: results on monitoring the health status of fish as bioindicator.

Highly anthropized areas as ports represent complex scenarios that require accurate monitoring plans aimed to address the environmental status. In this context, the activities of the EU Interreg Project "GEstione dei REflui per il MIglioramento delle Acque portuali (GEREMIA)" were focused on comparing sites differently affected by human presence, as the Port of Genoa and the natural area of the S'Ena Arrubia fishpond: a panel of analyses was carried out on Mugilidae fish sampled in these two areas, aimed to address trace metal accumulation in the liver, gills, and muscle, as well as cytochrome P450 (CYP450) induction in liver and biliary polycyclic aromatic hydrocarbon (PAH) metabolites, and histopathological alterations in the liver and gills. Chemical analyses in the liver, gills, and muscle of specimens collected in the port area showed an overall higher degree of trace metal contamination compared to the natural fishpond, and similar results were obtained in terms of CYP450 induction and biliary PAH metabolites, suggesting a higher exposure to organic compounds. In addition, histopathological analyses revealed a significant alteration and then a loss of functionality of liver and gill tissue in individuals from the port. Overall, this study describes the complex environmental pollution scenario in the Port of Genoa, confirming the importance of using multidisciplinary approaches and different types of analyses to address both the presence and the effects of contaminants in marine environments.

Mugilidae fish as bioindicator for monitoring plastic pollution: Comparison between a commercial port and a fishpond (north-western Mediterranean Sea).

In the last decade, interest in monitoring and managing plastic pollution has greatly increased. This study compared levels of microplastic contamination in stomachs of Mugilidae fish, suggesting this family as a target for plastic pollution monitoring in areas with different degrees of anthropisation. Two sites characterised by low and high anthropic impact, a fishpond (S'Ena Arrubia, Italy) and a port (Genoa, Italy), respectively, were compared. This study highlighted a stronger microplastic contamination in the port, with a higher percentage of fish showing the presence of microplastics and a larger polymeric variability compared to the fishpond. The microplastic number in fish from the port was higher than in the literature, but it was not significantly different from S'Ena Arrubia in terms of the microplastic percentage found in single individuals. Biomonitoring of microplastic contamination in Mugilidae fish resulted in a valid tool for the investigation of areas differently affected by human activity.

Microplastics in the abyss: a first investigation into sediments at 2443-m depth (Toulon, France).

Plastic and microplastic pollutions are known to be widespread across the planet in all types of environments. However, relatively little about microplastic quantities in the deeper areas of the oceans is known, due to the difficulty to reach these environments. In this work, we present an investigation of microplastic (<5 mm) distribution performed in the bottom sediments of the abyssal plain off the coast and the canyon of Toulon (France). Four samples of deep-sea sediment were collected at the depth of 2443 m during the sea operations carried out by the French oceanographic cruises for the KM3NeT project. The chemical and physical characterisation of the sediment was carried out, and items were extracted from sediments by density separation and analysed by optical microscope and µRaman spectroscopy. Results show microplastics in the deep-sea sediments with a concentration of about 80 particles L-1, confirming the hypothesis of microplastics spread to abyssal sediments in the Mediterranean Sea.

Considerations on salts used for density separation in the extraction of microplastics from sediments.

Environmental contamination by plastics and microplastics is a recognised problem worldwide, and it is the focus of many research teams. In the quantification of microplastics in the environment (plastic items with dimensions between 1 µm and 5 mm), the search for shared and universally recognised protocols and methodologies is still ongoing. In this study, the use of a method for extracting microplastics from marine sediments based on density separation has been considered. Fifty studies were analysed to investigate the scenario of salts exploited during microplastic extraction. The most commonly used salts are NaCl (45.6%), ZnCl2 (19.3%) and NaI (17.5%). Considerations related to cost, availability, hazards of the salts and thus the repeatability of the related extraction method are reported. In light of the findings, NaCl remains the most readily usable, economical and effective salt for the extraction of microplastics from marine sediments.

Correction to: Microplastics in seawater: sampling strategies, laboratory methodologies, and identification techniques applied to port environment.

The article Microplastics in seawater: sampling strategies, laboratory methodologies, and identification techniques applied to port environment.

Microplastics in seawater: sampling strategies, laboratory methodologies, and identification techniques applied to port environment.

The European Interreg Italy-France 2014-2020 Maritime Project SPlasH! (Stop to Plastics in H2O!) focused on the study of microplastics (MPs) in the marine port environment to evaluate their presence, abundance, and mechanisms of diffusion to the open sea. In the framework of this project, a worldwide review of 74 studies was carried out, providing an overview of MP investigation techniques, focusing on sampling strategies, laboratory methodologies, and identification of MPs collected in seawater, and specifically evaluating their applicability to the marine port environment. Nets were the most commonly used device for MP surface sampling, but their use can be difficult in narrow spaces within the port basins, and they must be coupled to discrete sampling devices to cover all port basins. In the laboratory, density separation (NaCl, ZnCl2, NaI, sodium lauryl sulfate (SLS)), filtration (polycarbonate, polyamide, glass, cellulose, ANOPORE inorganic membrane filters), sieving, visual sorting, and digestion methods (acidic, enzymatic, alkaline, oxidative) were used to separate MPs from seawater. Digestion becomes essential with water samples with great inorganic and organic loads as deriving from a port. Although many studies are based only on visual MP identification under a microscope, analytical identification techniques unequivocally determine the particle nature and the identity of the plastic polymers and are necessary to validate the visual sorting of MPs. Fourier-transform infrared spectroscopy (FTIR) is the most used analytical identification technique.

The sponge fauna of the Seno Magdalena and Puyuhuapi Fjord (Chile), with a description of two new species.

The presence of fjords, islands and channels originating from glacial erosion and ice cap retreat, makes the Chilean benthic biodiversity difficult to explore and study. Our survey of this region allowed the identification of 29 Demospongiae species in total. Two of them are new to science and here described: Biemna lutea sp. nov., and Hamigera cleistochela sp. nov.. Two species (Clathria (Clathria) microxa and Lissodendoryx (Ectyodoryx) patagonica,) are new for the region and the Chilean fjords. Lissodendoryx (Ectyodoryx) patagonica was found for the second time after the original description by Ridley Dendy, 132 years ago. These results - considering the small number of species identified on the whole - are promising and confirm that the marine biodiversity of Chilean fjords is remarkable but not well known yet.

Baseline evaluation of metal contamination in teleost fishes of the Gulf of Tigullio (north-western Italy): Histopathology and chemical analysis.

Metals, whether essential (Cu, Zn, Cr, Fe, Mn) or non-essential (Al, As, Cd, Ni, Pb, Hg) for organism metabolism, occur naturally in the marine environment and their abundance can increase due to the presence of human activities. In this study, fish were used as bio-indicators, to determine a correlation between the bio-accumulation of metals in muscle and gill tissues and the health status of fish. The study area was the Gulf of Tigullio (north-western Italy), which is impacted by various sources of metal contamination. Histopathology served as a significant tool to investigate possible alterations in gills, one of the main organs involved in fish physiology. Results highlighted some correlations between certain metals (e.g. Pb, Ni) and gill alterations (e.g. epithelial hyperplasia, epithelial lifting), providing baseline data from a pool of different fish species, which can be used for comparison purposes in further studies.