The effect of salinomycin on the immune response of chicks.

The effect of salinomycin drug on the immune system of chickens was investigated. By using the recommended dose 60 mg/kg feed salinomycin was given as anticoccidial drug continuously for 49 days. There was no noticeable significant effect on the humoral and cell-mediated immune responses to Newcastle Disease Virus (NDV) Vaccine. A drastic immunosuppressive effect was manifested by using a dose of 120 mg/kg and characterized by lowered titers of antibodies to NDV, decrease in the relative weight of lymphoid organs, lowered mitogenic response of peripheral blood lymphocytes (PBL) to phytohaemagglutinin (PHA) with reduction of heterophils percentage, as well as reduced protection rate to intramuscular challenge with the velogenic viscerotropic (VV) strain of NDV.

Infectious tenosynovitis in broilers and broiler breeders in Egypt.

Two infectious tenosynovitis-producing viruses were isolated from tendon sheaths and synovial fluids of 59 broilers and 15 broiler breeders obtained from different flocks in Egypt during June to October 1983. The viruses grew well on the chorioallantoic membrane of developing chicken embryos, produced small localized white pock lesions with oedematous swellings at the inoculation sites and death of most of the embryos 72 to 96 hours post-inoculation. They also induced cytopathic effect in chicken embryo rough, Vero and MS cell lines. The viruses were neutralized by reovirus S1133 antiserum, both in tissue culture and on the chorioallantoic membrane. Inoculation of the viruses into 2-day-old broiler chicks via the foot pad, intramuscular and oral routes reproduced the disease with the development of characteristic clinical, pathological and serological responses. The infection was transmitted to in-contact control chicks. This is the first report of the disease and of the isolation and identification of the causative virus in Eqypt.