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1.
J Pharm Biomed Anal ; 48(5): 1430-40, 2008 Dec 15.
Article in English | MEDLINE | ID: mdl-18947954

ABSTRACT

Several related substances (RS4-RS10) were detected in lopinavir drug substance at levels ranging from 0.03% to 0.1% by employing gradient RP-HPLC. The related substances were identified by LC-MS analysis. These related substances were isolated and characterized by Mass, (1)H NMR and FT-IR spectral data. The separation was achieved on a YMC Pack ODS-AQ (250 mm x 4.6 mm, 5 microm) column thermostated at 45 degrees C using 0.02 M KH(2)PO(4) (pH 2.5): acetonitrile as a mobile phase in gradient elution mode. A PDA detector set at 210 nm was used for detection. The investigated validation elements showed the method has acceptable specificity, accuracy, linearity, precision, robustness and high sensitivity with detection limits and quantitation limits ranging from 0.028 microg/ml to 0.063 microg/ml and 0.084 microg/ml to 0.192 microg/ml respectively. The method can be used for routine quality control analysis and stability testing of lopinavir drug substance.


Subject(s)
Drug Contamination , HIV Protease Inhibitors/chemistry , Pharmaceutical Preparations/chemistry , Pyrimidinones/chemistry , Buffers , Chromatography, High Pressure Liquid/instrumentation , Chromatography, High Pressure Liquid/methods , Drug Stability , Guidelines as Topic , HIV Protease Inhibitors/analysis , HIV Protease Inhibitors/chemical synthesis , Hydrogen-Ion Concentration , Lopinavir , Mass Spectrometry , Molecular Structure , Nuclear Magnetic Resonance, Biomolecular , Pharmaceutical Preparations/analysis , Phosphates/chemistry , Potassium Compounds/chemistry , Pyrimidinones/analysis , Pyrimidinones/chemical synthesis , Quality Control , Reference Standards , Reproducibility of Results , Sensitivity and Specificity , Spectrometry, Mass, Electrospray Ionization/methods , Spectroscopy, Fourier Transform Infrared , Temperature , Time Factors , Water/chemistry
2.
J Pharm Biomed Anal ; 43(4): 1476-82, 2007 Mar 12.
Article in English | MEDLINE | ID: mdl-17174507

ABSTRACT

Three unknown impurities in Cefdinir bulk drug at levels below 0.2% (ranging from 0.05 to 0.2%) have been detected by high performance liquid chromatography (HPLC). These impurities were isolated from crude sample of Cefdinir using preparative HPLC. Based on the spectral data (NMR, IR and MS) the structures of these impurities were characterized as (6R, 7R)-7-[(z)-2-(2-aminothiazol-4-yl)-2-hydroxyiminoacetamido]-8-oxo-3-vinyl-5-thia-1-azabicyclo [4.2.0] oct-2-ene-2-carboxylic acid-5-oxide (I). (6R, 7R)-7-[(z)-2-(2-aminothiazol-4-yl)-2-hydroxyiminoacetamido]-8-oxo-3-vinyl-5-thia-1-azabi-cyclo [4.2.0] oct-3-ene-2-carboxylic acid (II). (6R, 7R)-7-[(z)-2-(2-aminothiazol-4-yl)-2-hydroxyiminoacetamido]-8-oxo-3-methyl-5-thia-1-azabicyclo-[4.2.0]oct-2-ene-2-carboxylic acid (III), respectively. The origin and structural elucidation of all impurities have been discussed.


Subject(s)
Anti-Bacterial Agents/analysis , Cephalosporins/analysis , Drug Contamination , Anti-Bacterial Agents/chemistry , Anti-Bacterial Agents/standards , Cefdinir , Cephalosporins/chemistry , Cephalosporins/standards , Chromatography, High Pressure Liquid , Magnetic Resonance Spectroscopy , Mass Spectrometry , Molecular Structure , Spectroscopy, Fourier Transform Infrared
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