ABSTRACT
Monoparacoumarylputrescinium chloride (pCPH), supplied to detached leaves of Gomphrena globosa via the petiole, induced interference with tomato bushy stunt virus infection by reducing the number and size of the necrotic local lesions. The phenolic compound neither inactivated directly the virus in vitro, nor induced interference when supplied just after virus inoculation, all this indicating an effect on cell metabolism. The interference was positively correlated to both pCPH concentration and time of induction (viz., the intervals between pCPH supply and inoculation). Coumaric acid did not, whereas putrescine, but not spermidine or spermine, did induce interference. Disc electrophoresis in polyacrylamide gels revealed no changes in the soluble protein constitution between pCPH-treated and control leaves.
