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1.
J Clin Invest ; 116(6): 1514-24, 2006 Jun.
Article in English | MEDLINE | ID: mdl-16691294

ABSTRACT

HIV infection selectively targets CD4+ effector memory T (T EM) cells, resulting in dramatic depletion of CD4+ T cells in mucosal effector sites in early infection. Regeneration of the T EM cell compartment is slow and incomplete, even when viral replication is controlled by antiretroviral therapy (ART). Here, we demonstrate that IL-15 dramatically increases in vivo proliferation of rhesus macaque (RM) CD4+ and CD8+ T EM cells with little effect on the naive or central memory T (T CM) cell subsets, a response pattern that is quite distinct from that of either IL-2 or IL-7. T EM cells produced in response to IL-15 did not accumulate in blood. Rather, 5-bromo-2'-deoxyuridine (BrdU) labeling studies suggest that many of these cells rapidly disperse to extralymphoid effector sites, where they manifest (slow) decay kinetics indistinguishable from that of untreated controls. In RMs with uncontrolled SIV infection and highly activated immune systems, IL-15 did not significantly increase CD4+ T EM cell proliferation, but with virologic control and concomitant reduction in immune activation by ART, IL-15 responsiveness was again observed. These data suggest that therapeutic use of IL-15 in the setting of ART might facilitate specific restoration of the CD4 + T cell compartment that is the primary target of HIV with less risk of exhausting precursor T cell compartments or generating potentially deleterious regulatory subsets.


Subject(s)
CD4-Positive T-Lymphocytes/immunology , Cell Movement/physiology , Immunologic Memory , Interleukin-15/immunology , T-Lymphocyte Subsets/immunology , Animals , Anti-Retroviral Agents/immunology , Anti-Retroviral Agents/therapeutic use , CD28 Antigens/immunology , CD8-Positive T-Lymphocytes/immunology , Cell Proliferation , HIV Infections/immunology , Humans , Interleukin-15/therapeutic use , Interleukin-2/immunology , Interleukin-7/immunology , Lymphocyte Activation , Macaca mulatta , Male , Receptors, CCR7 , Receptors, Chemokine/immunology , Simian Acquired Immunodeficiency Syndrome/drug therapy , Simian Acquired Immunodeficiency Syndrome/immunology , Viral Load , Virus Replication
2.
J Exp Med ; 200(10): 1299-314, 2004 Nov 15.
Article in English | MEDLINE | ID: mdl-15545355

ABSTRACT

The mechanisms linking human immunodeficiency virus replication to the progressive immunodeficiency of acquired immune deficiency syndrome are controversial, particularly the relative contribution of CD4+ T cell destruction. Here, we used the simian immunodeficiency virus (SIV) model to investigate the relationship between systemic CD4+ T cell dynamics and rapid disease progression. Of 18 rhesus macaques (RMs) infected with CCR5-tropic SIVmac239 (n=14) or CXCR4-tropic SIVmac155T3 (n=4), 4 of the former group manifested end-stage SIV disease by 200 d after infection. In SIVmac155T3 infections, naive CD4+ T cells were dramatically depleted, but this population was spared by SIVmac239, even in rapid progressors. In contrast, all SIVmac239-infected RMs demonstrated substantial systemic depletion of CD4+ memory T cells by day 28 after infection. Surprisingly, the extent of CD4+ memory T cell depletion was not, by itself, a strong predictor of rapid progression. However, in all RMs destined for stable infection, this depletion was countered by a striking increase in production of short-lived CD4+ memory T cells, many of which rapidly migrated to tissue. In all rapid progressors (P <0.0001), production of these cells initiated but failed by day 42 of infection, and tissue delivery of new CD4+ memory T cells ceased. Thus, although profound depletion of tissue CD4+ memory T cells appeared to be a prerequisite for early pathogenesis, it was the inability to respond to this depletion with sustained production of tissue-homing CD4+ memory T cells that best distinguished rapid progressors, suggesting that mechanisms of the CD4+ memory T cell generation play a crucial role in maintaining immune homeostasis in stable SIV infection.


Subject(s)
CD4-Positive T-Lymphocytes/immunology , Immunologic Memory , Simian Acquired Immunodeficiency Syndrome/immunology , Simian Acquired Immunodeficiency Syndrome/physiopathology , Simian Immunodeficiency Virus/pathogenicity , Analysis of Variance , Animals , Bromodeoxyuridine , CD4 Lymphocyte Count , CD4-Positive T-Lymphocytes/metabolism , Disease Progression , Flow Cytometry , Fluorescent Antibody Technique , Macaca mulatta , Male , Species Specificity
3.
Blood Cells Mol Dis ; 30(2): 177-83, 2003.
Article in English | MEDLINE | ID: mdl-12732181

ABSTRACT

The t(8;21) is one of the most frequent chromosomal translocations associated with acute leukemia. The translocation fuses the DNA binding domain of AML1 to nearly all of the ETO co-repressor. ETO associates with the mSin3 and N-CoR co-repressors as well as histone deacetylases 1, 2, and 3. Although this is one of the most frequent chromosomal translocations in acute leukemia, accounting for 10-15% of the cases of acute myeloid leukemia (AML), the direct targets for transcriptional regulation that stimulate leukemogenesis are unknown. We found that AML1-ETO repressed the promoter of p14(ARF) tumor suppressor in transient transfection assays and reduced endogenous levels of p14(ARF) expression in multiple cell types. Chromatin immunoprecipitation assays demonstrated that AML1-ETO bound to the p14(ARF) promoter. In acute myeloid leukemia samples containing the t(8;21), levels of p14(ARF) mRNA were markedly lower when compared to other acute myeloid leukemias. Therefore, p14(ARF) is a direct transcriptional target of AML1-ETO.


Subject(s)
Chromosomes, Human, Pair 21 , Chromosomes, Human, Pair 8 , Gene Expression Regulation, Neoplastic , Histone Deacetylases/genetics , Transcription, Genetic , Translocation, Genetic , Tumor Suppressor Protein p14ARF/genetics , Acute Disease , Apoptosis , Cell Division , Humans , Leukemia, Myeloid/genetics , Leukemia, Myeloid/pathology , Oncogenes , Repressor Proteins/genetics
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