ABSTRACT
Thirty-nine bacterial strains, obtained from commercial starter cultures and commonly used by the meat industry in Spain, have been examined for their ability to produce bacteriocins. Fourteen (35%) of them showed antagonism against at least one of the indicator strains, by the agar spot test. The strains showing an inhibitory action against pathogenic indicator strains were identified as Pediococcus acidilactici, Lactobacillus curvatus, Lactobacillus pentosus and Lactobacillus plantarum, which showed an inhibitory action against a wide range of Gram-positive bacteria. The only strain which showed reliable inhibitory activity against pathogenic indicator strains, by the well diffusion assay, was P. acidilactici. This strain produces an inhibitory compound, which reaches its maximum activity at the beginning of the stationary phase of growth. This antimicrobial substance (bacteriocin) has a proteinaceous nature, is stable over a broad range of pH, resistant to heat and shows a bactericidal action.
Subject(s)
HIV Infections/transmission , HIV Seropositivity/epidemiology , Inpatients , Mental Disorders/epidemiology , Comorbidity , Cross-Sectional Studies , HIV Antibodies/analysis , Hospitals, Psychiatric , Humans , Sexual Behavior , Spain , Substance Abuse, Intravenous/complications , Time FactorsSubject(s)
Adenoviridae Infections/microbiology , Adenoviruses, Human/isolation & purification , Diarrhea/microbiology , Feces/microbiology , Latex Fixation Tests , Adenoviruses, Human/ultrastructure , Child , Child, Preschool , Diarrhea, Infantile/microbiology , Humans , Infant , Microscopy, Electron , Reproducibility of ResultsABSTRACT
Adenovirus (ADV) and rotavirus (RV) were screened by latex aglutination (LA) in 772 fecal samples from hospitalized and non hospitalized children under 7 years of age, those included 55 control samples. Reactive samples were confirmed by electron microscopy (ME) and enzimoimmunoassay (EIA). ADV were found in 20 (2.6%) samples and RV in 25 (3.2%). Coinfection by both viruses was not demonstrated. The greatest percentage of findings was observed in watery (23% ADV and 25% RV) and almost watery stools (8% ADV and 25% RV). We did not find any of those viruses in samples from control children. Concordance between LA and confirmation methods used was excellent. Thus the LA tests could provide a simple tool for the diagnosis of non hospitalized patients, increasing the possibilities of a rapid ethiological diagnostic in infectious diarrhoeas.
