Effects of dry period length on milk production, body condition, metabolites, and hepatic glucose metabolism in dairy cows.

Dry period (DP) length affects energy metabolism around calving in dairy cows as well as milk production in the subsequent lactation. The aim of the study was to investigate milk production, body condition, metabolic adaptation, and hepatic gene expression of gluconeogenic enzymes in Holstein cows (>10,000 kg milk/305 d) with 28- (n=18), 56- (n=18), and 90-d DP (n=22) length (treatment groups) in a commercial farm. Cows were fed total mixed rations ad libitum adjusted for far-off (not for 28-d DP) and close-up DP and lactation. Milk yield was recorded daily and body condition score (BCS), back fat thickness (BFT), and body weight (BW) were determined at dry off, 1 wk before expected and after calving, and on wk 2, 4, and 8 postpartum (pp). Blood samples were taken on d -56, -28, -7, 1, 7, 14, 28, and 56 relative to calving to measure plasma concentrations of metabolites and hormones. Liver biopsies (n=11 per treatment) were taken on d -10 and 10 relative to calving to determine glycogen and total liver fat concentration (LFC) and to quantify mRNA levels of pyruvate carboxylase (PC), cytosolic phosphoenolpyruvate carboxykinase, and glucose-6-phosphatase. Time course of milk yield during first 8 wk in lactation differed among treatment. Milk protein content was higher in 28-d than in 90-d DP cows. Milk fat to protein ratio was highest and milk urea was lowest in 90-d DP cows. Differences in BW, BFT, and BCS were predominantly seen before calving with greatest BW, BFT, and BCS in 90-d DP cows. Plasma concentrations of NEFA and BHBA were elevated during the transition period in all cows, and the greatest increase pp was seen in 90-d DP cows. Plasma glucose concentration decreased around calving and was greater in 28-d than in 90-d DP cows. Dry period length also affected plasma concentrations of urea, cholesterol, aspartate transaminase, and glutamate dehydrogenase. Plasma insulin concentration decreased around calving in all cows, but insulin concentration pp was greater in 28-d than in 56-d DP cows. Hepatic glycogen concentration decreased and LFC increased after calving in all cows, and LFC was greater pp in 90-d DP than in 28-d DP cows. Hepatic PC mRNA abundance pp tended to increase most in 90-d DP cows. Changes on glucose metabolism were more balanced in cows with a reduced DP, whereas cows with extended DP and elevated body condition indicated greatest metabolic changes according to lipid and glucose metabolism during the transition period.

[Comparative studies of superovulation in high-producing cows]. / Vergleichende Untersuchungen zur Superovulation bei Hochleistungskühen.

PMSG-PG (1), PMSG-PG-anti-PMSG (2), and FSH-PG (3) were used under production conditions for superovulation treatment of high-yielding cows. An analysis of results has shown the FSH variant (3) to be superior with significance to the other two regimes. It yielded positive ovarian reactions (greater than or equal to 3 corpora lutea) in 92.0 percent of all cases, with 7.0 +/- 6.3 transferable embryos being obtained. Regime 1 gave only 75.5 percent as well as 3.2 +/- 2.97, while 85.5 percent and 4.3 +/- 3.9 were recorded from regime 2. Significance amounted to P less than 0.01; 0.001. Regimes 2 and 3 in standardised form are recommended for embryo transfer with cattle in breeding practice.