ABSTRACT
Listeria monocytogenes is the third most deadly foodborne pathogen in the United States. The bacterium is found in soil and water, contaminating raw food products and the processing environment, where it can persist for an extended period. Currently, testing of food contact and non-food contact surfaces is performed using an array of sampling devices and endpoint technologies, offering various levels of sensitivity, cost, user skill, and time to detection. Paper-based microfluidic devices (µPADs) are a rapid detection platform amenable to low-cost, user-friendly, and portable diagnostics. In this study, we developed and evaluated a µPAD platform specific for the colorimetric detection of the Listeria genus following recovery from food contact and non-food contact surfaces. For detection, four colorimetric substrates specific for the detection of ß-glucosidase, two broths selective for the detection of Listeria spp., and a nonselective broth were evaluated to facilitate detection of Listeria spp. The limit of detection and time to detection were determined by using pure bacterial cultures. After 8 h enrichment, L. monocytogenes (102 Colony Forming Units (CFU)/coupon) was detected on every surface. After 18 h enrichment, L. monocytogenes (102 CFU/coupon) was detected on all surfaces with all swabbing devices. This study demonstrated the ability of the µPAD-based method to detect potentially stressed cells at low levels of environmental contamination.
ABSTRACT
A method for lead (Pb) detection in soil is presented. Pb is a dangerous environmental pollutant that is present in soils, posing a health risk to millions of people worldwide, and regular monitoring of Pb contamination in soils is essential to public health. Many sensitive methods for detection of heavy metals in solid matrices exist, but they cannot be performed on-site because they are costly (>$30 per sample), require trained personnel, and many classical sample preparation methods are not safe to bring into the field. We describe an alternative process, combining a safer sample preparation method with electrochemical analysis. The process requires minimal training, making it an attractive overall method for regular environmental screening of Pb in soils. Extract obtained from the soil is pH adjusted and analyzed using a stencil-printed carbon electrode and square wave anodic stripping voltammetry. In this work, a study of 15 neighborhood soils examining the concentration of Pb present post-extraction was performed to demonstrate the method. The limit of detection for the electrochemical analysis was calculated to be 16 ppb-well below the United States Environmental Protection Agency's action limit for Pb in soils (400 mg kg-1 or 4000 ppb)-and third party inductively coupled plasma-optical emission spectroscopy analysis validated the results obtained in this study to within ±17% on average.
Subject(s)
Metals, Heavy , Soil Pollutants , Environmental Monitoring , Environmental Pollution , Humans , Lead , Metals, Heavy/analysis , Soil , Soil Pollutants/analysisABSTRACT
The field of paper-based microfluidics has experienced rapid growth over the past decade. Microfluidic paper-based analytical devices (µPADs), originally developed for point-of-care medical diagnostics in resource-limited settings, are now being applied in new areas, such as environmental analyses. Low-cost paper sensors show great promise for on-site environmental analysis; the theme of ongoing research complements existing instrumental techniques by providing high spatial and temporal resolution for environmental monitoring. This review highlights recent applications of µPADs for environmental analysis along with technical advances that may enable µPADs to be more widely implemented in field testing.
Subject(s)
Chemistry Techniques, Analytical/methods , Environment , Paper , Animals , Chemistry Techniques, Analytical/instrumentation , HumansABSTRACT
This contribution describes the synthesis and characterization of a perylene diimide (PDI)-based n-type semiconductor and its application to organic photovoltaic (OPV) devices having inverted architecture. Films of N,N'-bis(3-trimethoxysilylpropyl)-1,6,7,12-tetrachloroperylene-3,4,9,10-tetracarboxyldiimide (Cl(4)PSi(2)) and blends of this material with various polymers are solution-deposited on tin-doped indium oxide (ITO) substrates as interfacial layers (IFLs). The organic IFL described in this work is based on the air- and light-stable PDI core, annealed at low temperatures compatible with flexible substrates, and crosslinks in air for compatibility with device fabrication. Morphological, optical, and electrochemical analysis of these IFL films demonstrate predominantly smooth surfaces and HOMO and LUMO energies of ~4.5 and 7.0 eV, respectively, which are ideal for accepting electrons and blocking holes in inverted devices. A cationic silane species is added to the Cl(4)PSi(2) at an optimum ~2-5 wt % to reduce IFL series resistance and enhance device performance. Also, a short light soaking procedure is necessary for completed devices to achieve high fill factors in current density-voltage analysis, a phenomenon previously only observed for inverted devices having an n-type inorganic IFL.
ABSTRACT
Disordered nanohole arrays were formed in silver films by colloidal lithography techniques and characterized for their surface-plasmon activity. Careful control of the reagent concentration, deposition solution ionic strength, and assembly time allowed generation of a wide variety of nanohole densities. The fractional coverage of the nanospheres across the surface was varied from 0.05-0.36. Electrical sheet resistance measurements as a function of nanohole coverage fit well to percolation theory indicating that the electrical behavior of the films is determined by bulk silver characteristics. The transmission and reflection spectra were measured as a function of coverage and the results indicate that the optical behavior of the films is dominated by surface plasmon phenomena. Angle-resolved transmission and reflection spectra were measured, yielding insight into the nature of the excitations taking place on the metal films. The tunability of the colloidal lithography assembly method holds much promise as a means to generate customized transparent electrodes with high surface plasmon activity throughout the visible and NIR spectrum over large surface areas.
Subject(s)
Nanostructures/chemistry , Optical Phenomena , Silver/chemistry , Colloids , Electricity , Nanospheres/chemistry , Salts/chemistry , Spectrophotometry, Infrared , Time FactorsABSTRACT
In organic solar cells, the efficiency of the exciton transport and dissociation across donor-acceptor (D/A) interfaces is controlled by the nanoscale distribution of the donor and acceptor phases. The observation of photoluminescence quenching is often used as confirmation for efficient exciton dissociation but provides no information on the nanoscopic nature of the exciton transport. Here we demonstrate nanoscale imaging of the exciton transport in films consisting of the conjugated polymer poly(3-hexylthiophene) (P3HT, electron donor) blended with the C60 derivative 1-(3-methoxycarbonyl)-propyl-1-phenyl-(6,6)C61 (PCBM, electron acceptor) by a tunneling luminescence spectroscopy based on atomic force microscopy. The excitonic luminescence is significantly enhanced when the conjugated polymer is coupled to the plasmon excitation at the tip (tip-enhanced luminescence). This effect allows one to dramatically improve the detection efficiency of the excitonic luminescence and, consequently, resolve individual domains of the conjugated polymer in which the exciton will recombine before dissociation at the D/A interface. Under thermal annealing conditions promoting the segregation of the donor and acceptor phases, a clear increase of the luminescence is seen from polymer-rich regions, consistent with domains of dimensions much larger than the exciton diffusion length. The described scanning luminescence microscopy can thus be applied to the optimization of the blends used in solar cells.
ABSTRACT
The design and optimization of a vapor-phase analyte deposition method for limit of detection (LOD) studies on engineered surface-enhanced Raman scattering (SERS)-active substrates is presented. The vapor deposition method was designed to overcome current challenges in quantitative analysis of lithographically produced SERS substrates that are relatively small (hundreds of square micrometers). A custom-built flow cell was used to deposit benzenethiol from the vapor phase onto SERS-active Ag thin films, as the control substrates, and nanoaperture arrays that were generated by electron-beam lithography. The surface coverage of benzenethiol as a function of time was monitored using the ring stretching mode 1070-cm(-1) band and the trend was fit to Langmuir adsorption kinetics. The method was deemed reliable based on agreement between the LOD determined on the control substrates and previously reported values for those substrates. Application of the new method to a 20 x 20 microm(2) nanoaperture array yielded a LOD of 4.2 +/- 0.3 amol.