Information fields of written texts protect cells from oxidative damage and accelerate repair.

OBJECTIVE: Written words demonstrated specific effects on biochemical measures and cell growth in cultured mammalian cells in previous study. We examined the protection and reparative effects of Chinese texts with positive meaning and the word "Buddha" on oxidative-damaged cells, with the goal of exploring the therapeutic effect of such characters. METHODS: The human embryonic kidney cell line HEK293T was utilized, with the oxidative- damaged cell model produced by hydrogen peroxide (H2O2). Double-blind experiments were set up to ensure all operators involved in the experiments did not know the contents of the texts in envelopes. Petri-dishes (9 plates) containing oxidative-damaged cells were selected randomly for three controls, three treatments by texts with positive meaning, and another three treatment by the word "Buddha". Cells were sub-sampled at 3 h and 24 h to examine growth and mitochondrial functions. All data are expressed relative to the controls. RESULTS: Compared with the control group, mitochondrial functions were enhanced as evidenced by increased adenosine triphosphate (ATP) levels, accompanied by the significant improvement in cell growth rate for the groups treated by positive texts and the word "Buddha". Reactive oxygen species (ROS) levels were decreased significantly in positive texts treatment, which suggested that the antioxidant capacity of cells was also improved. CONCLUSIONS: This study revealed that certain texts can potentially offer protection and accelerate the cellular repair for oxidative-damaged cells.

Effects of different music on HEK293T cell growth and mitochondrial functions.

BACKGROUND: Mitochondria are considered a portal to receive, process and integrate external energy and information to maintain cellular homeostasis. Previous studies demonstrated that mitochondrial function and antioxidant capacity of in-vitro cultured mammalian cells is modified by different energetic stimuli including electromagnetic energy, acoustic energy, external Qi and subtle energy emitted by written texts in relatively short time frames. Thus, individual cells can act as a rapid and sensitive biological sensor and act as a platform to evaluate and understand the subtle effect of different biophysical stimuli. Regarding acoustic energy, several different types of music have been reported to produce beneficial effects on human health. OBJECTIVE: To compare eastern and western music styles on cell function at the biochemical level to understand the underlying mechanisms involved. Methods In this study, we compared the effects of Chinese five-element music with two types of western music (heavy-metal and classical) on mitochondrial function, oxidative capacity and growth using HEK293T cells (human embryonic kidney cells). RESULTS: Unlike rock and classical music, exposing cells to five-element music produced several beneficial physiological effects, including statistically significant increases in the production of adenosine triphosphate (ATP) by 17%, glutathione (GSH) by 21% and cell growth rates (14%), as well as a significant reduction in the reactive oxygen species (ROS) by 13%. For the group treated with classical music, there was only a trend toward increased GSH (8%), although the increased growth rates (14%) did reach significance. In sharp contrast, the cells treated with heavy-metal music responded with an opposite and significant 16% increase in ROS and a significant 11% reduction in cell viability. CONCLUSIONS: This study revealed the dramatically different and even opposite effects of different styles of music on specific biochemical measures in cultured human cells. These results help explain the underlying biochemical mechanisms of the effects of the different types of music.

Effect of information fields from written texts on cell growth and mitochondrial functions in-vitro: An exploratory study.

OBJECTIVE: Mitochondria are considered a portal to receive, process and integrate external energy and information to maintain cellular homeostasis. We examined the effect of Chinese texts with positive and negative meaning on the growth and mitochondrial functions using a mouse kidney collecting duct cell line called M1 cells. METHODS: To avoid skewing the results due to differential handling of the cells or analyzing the results, we conducted experiments by keeping the texts and blanks covered in brown opaque envelopes, exposed the cells to randomly selected envelopes and examined the differences over time. All operators involved in the experiments did not know the contents of the envelopes until the end of the experiments, and all data are expressed relative to the controls. RESULTS: Cell growth rate was not affected for the group treated with positive information but was significantly reduced by 18% when treated with negative information. At the biochemical level, positive texts significantly increased whole cell adenosine triphosphate (ATP) and glutathione (GSH) by 22% and 21% respectively. CONCLUSIONS: This study for the first time demonstrated the effect of written words on specific biochemical measures in cultured mammalian cells.

Rate limiting factors for DNA transduction induced by weak electromagnetic field.

DNA transduction across aqueous solutions has been reported previously. In this study, we examined a few key factors affecting DNA transduction rate in an extremely low frequency electromagnetic field. These include: the chemical composition of the aqueous solutions, the type of experimental vessel, the dilution step, and the origin of the DNA fragments. The results indicate that partially introducing essential ingredients for DNA amplification (i.e. dNTPs and PCR buffer) to the aqueous solution enhanced the transduction rate greatly, and transduction vessels made of hydrophilic quartz yielded more favorable results than vessels made of hydrophobic plastic. In addition, performing a serial dilution to the transduction solution more than doubled the transduction rate compared to that without the dilution step. For the DNA fragments used in this study, there was one with a pathogenic origin and two with non-pathogenic origins. However, all three fragments achieved DNA transduction regardless of the difference in their origins. The experimental setup for eliminating the false positives caused by both biological and potentially physical contamination is also described.

A mechanistic model for the aging of human skin.

The microinflammatory model of skin aging is described. This model accounts for the loss of elasticity, resiliene and flexibility of the dermis, as well as for the appearance of wrinkles and for the thinning of the epidermis which are associated with aging. The lack of appropriate apparatuses for the correct measurements of skin hydration does not allow one to test this model for its predictive capability of the appearance of dry skin with age. The micro-inflammatory model of skin aging fails to predict the appearance of age spots on the surface of the skin.

Bioinformation within the biofield: beyond bioelectromagnetics.

This review article extends previous scientific definitions of the biofield (endogenous energy fields of the body) to include nonclassical and quantum energy fields. The biofield is defined further in terms of its functional property to act as a resonance target for external forms of energy used as treatment modalities in energy medicine. The functional role of the biofield in the body's innate self-healing mechanisms is hypothesized, based on the concept of bioinformation which, mediated by consciousness, functions globally at the quantum level to supply coherence, phase, spin, and pattern information to regulate and heal all physiologic processes. This model is used to explain a wide variety of anomalies reported in the scientific literature, which can not be explained by traditional biophysics and bioelectromagnetics.