The structure of the capsid inner layer of reoviruses. Brief report.

It is shown that reovirus capsid inner layer consists of 132 capsomeres with a skewed icosahedral pattern of T = 13 (left). 780 structural units composing capsomeres are integrated into trimers. The boundary between capsomeres runs between the structural units forming trimers.

Light and electron microscope study of the neurotropism of Powassan virus strain P-40.

Brains of adult white mice inoculated with the P-40 strain of Powassan virus isolated in Primorsky Krai (U.S.S.R) from ticks were studied by light and electron microscopy. Accumulations of virus particles were found in neurons and their dendrites and axons, in glial cells, and in intercellular spaces. In the nerve cells, most prevalent were changes of the type of chromatolysis and formation of small vacuoles, associated with the alteration of the endoplasmic reticulum induced by virus morphogenesis. In virus-affected cells, multilayer dense membranes were found.

Experimental Hazara Virus infection in mice.

Reproduction of Hazara virus in intracerebrally (ic) inoculated newborn white mice was studied by virological, serological and histological methods, electron microscopy and the direct fluorescent antibody technique. In the course of infection the virus was detectable in the blood, brain and some viscera, the largest amounts of virus occurring in the brain and liver. Pathomorphological lesions were localized in the brain and consisted of foci of neuronal destruction and marked oedema of the tissue. Electron microscopy of ultrathin sections of tissues from various parts of brains of infected newborn white mice killed at the height of infection revealed virus particles in perikaryon of the brain cortex neurons; accumulations of particles were found in vesicles of the Golgi complex and the endoplasmic reticulum.