Prostate cancer tissue classification by multiphoton imaging, automated image analysis and machine learning.

Prostate carcinoma, a slow-growing and often indolent tumour, is the second most commonly diagnosed cancer among men worldwide. The prognosis is mainly based on the Gleason system through prostate biopsy analysis. However, new treatment and monitoring strategies depend on a more precise diagnosis. Here, we present results by multiphoton imaging for prostate tumour samples from 120 patients that allow to obtain quantitative parameters leading to specific tumour aggressiveness signatures. An automated image analysis was developed to recognise and quantify stromal fibre and neoplastic cell regions in each image. The set of metrics was able to distinguish between non-neoplastic tissue and carcinoma areas by linear discriminant analysis and random forest with accuracy of 89% ± 3%, but between Gleason groups of only 46% ± 6%. The reactive stroma analysis improved the accuracy to 65% ± 5%, clearly demonstrating that stromal parameters should be considered as additional criteria for a more accurate diagnosis.

Care to the dependent older person: motivations of caregivers in Brazil and Portugal / O cuidado ao idoso dependente: motivações de cuidadores do Brasil e Portugal / Cuidando a los dependientes mayores: motivaciones de cuidadores en Brasil y Portugal

In an attempt to fulfill care needs to the elderly, the family usually assumes this function. In order to understand the social representations of informal caregivers of Brazilian and Portuguese elderly people about the motivations for care, a qualitative, exploratory-descriptive research was carried out, based on the Social Representations Theory, in which 21 informal Brazilian caregivers and 11 informal Portuguese caregivers of elderly dependents were interviewed. Thematic Content Analysis and QSR NVivo® software were used in the treatment of the data, and the results showed care as the main motivation: family relationships, in which marriage, parenting, and the affections resulting from these relationships led to the function; the negative representation of Portuguese caregivers on the home, as a place of abandonment and action contrary to the cultural norm; and the representation of the elderly as frail and dependent, who, like children, constantly need care and attention.

Na tentativa de suprir as necessidades de cuidado destinado ao idoso, a família comumente é a instituição que assume tal função. Com o objetivo de compreender as representações sociais dos cuidadores informais de idosos brasileiros e portugueses acerca das motivações para o cuidado, realizou-se uma pesquisa qualitativa, exploratória e descritiva, fundamentada na Teoria das Representações Sociais, em que foram entrevistados 21 cuidadores informais brasileiros e 11 cuidadores informais portugueses de idosos dependentes. Utilizaram-se a análise de conteúdo temática e o software QSR NVivo® no tratamento dos dados, e os resultados evidenciaram como principais motivações o cuidado: as relações familiares, em que o matrimônio, a parentalidade e os afetos decorrentes dessas relações conduziram a função; a representação negativa dos cuidadores portugueses sobre o lar como local de abandono e ação contrária à norma cultural; e a representação da pessoa idosa como frágil e dependente, que, assim como as crianças, precisa constantemente de cuidados e atenção.

En un intento por atender las necesidades de cuidado de las personas mayores, la familia suele ser la institución que asume esta función. Para comprender las representaciones sociales de los cuidadores informales de ancianos brasileños y portugueses sobre las motivaciones del cuidado, se realizó una investigación cualitativa, exploratoria-descriptiva, basada en la Teoría de las Representaciones Sociales, en la que 21 cuidadores informales brasileños y 11 informales portugueses Se entrevistó a los cuidadores de ancianos dependientes. En el tratamiento de los datos se utilizó el software de Análisis de Contenido Temático y QSR NVivo® y los resultados se mostraron como las principales motivaciones: las relaciones familiares, en las que el matrimonio, la paternidad y los afectos resultantes de estas relaciones condujeron a la función; la representación negativa de los cuidadores portugueses en el hogar, como lugar de abandono y acción contraria a la norma cultural; y la representación de los ancianos como frágiles y dependientes, quienes, como los niños, necesitan cuidados y atenciones constantes.

Canine mammary cancer tumour behaviour and patient survival time are associated with collagen fibre characteristics.

Precise diagnosis and prognosis are key in prevention and reduction of morbidity and mortality in all types of cancers. Here we show that changes in the collagen fibres in the main histological subtypes of canine mammary gland carcinomas are directly associated with the tumour behaviour and the animal survival time and could become a useful tool in helping with diagnosis. Imaging by second harmonic generation and multiphoton excited fluorescence microscopy were performed to evaluate the collagen and cellular segment parameters in cancer biopsies. We present a retrospective study of 45 cases of canine mammary cancer analysing 836 biopsies regions including normal mammary gland tissue, benign mixed tumours, carcinoma in mixed tumour, carcinosarcoma, micropapillary carcinoma and solid carcinoma. The image analyses and the comparison between the tumour types allowed to assess the collagen fibre changes during tumour progression. We demonstrate that the collagen parameters correlate with the clinical and pathological data, the results show that in neoplastic tissues, the collagen fibres are more aligned and shorter as compared to the normal tissues. There is a clear association of the mean fibre length with the dogs survival times, the carcinomas presenting shorter collagen fibres indicate a worse survival rate.

Canine mammary cancer diagnosis from quantitative properties of nonlinear optical images.

We present nonlinear microscopy imaging results and analysis from canine mammary cancer biopsies. Second harmonic generation imaging allows information of the collagen structure in the extracellular matrix that together with the fluorescence of the cell regions of the biopsies form a base for comprehensive image analysis. We demonstrate an automated image analysis method to classify the histological type of canine mammary cancer using a range of parameters extracted from the images. The software developed for image processing and analysis allows for the extraction of the collagen fibre network and the cell regions of the images. Thus, the tissue properties are obtained after the segmentation of the image and the metrics are measured specifically for the collagen and the cell regions. A linear discriminant analysis including all the extracted metrics allowed to clearly separate between the healthy and cancerous tissue with a 91%-accuracy. Also, a 61%-accuracy was achieved for a comparison of healthy and three histological cancer subtypes studied.

Quality of life and associated factors for caregivers of functionally impaired elderly people.

BACKGROUND: Lifestyle changes, factors inherent to the caregiver's family and social environment, and physical and mental aspects determine a caregiver's quality of life (QOL). OBJECTIVE: To evaluate the QOL and associated factors for caregivers of functionally impaired elderly people. METHOD: This was a cross-sectional study that analyzed data from a home-based care survey. The study sites were the homes of elderly people enrolled in Family Health Centers (Unidades de Saúde da Família) in Jequié, Bahia, Brazil. The sample consisted of 40 caregivers of functionally impaired elderly people. The instrument consisted of demographic data, health status, the Perceived Family Support Inventory, the Social Support Satisfaction Scale, and the World Health Organization Quality of Life Assessment (WHOQOL-BREF). The data were analyzed using a descriptive analysis and the chi-squared test at a 5% significance level, performed in SPSS version 15.0. RESULTS: An association was found between the WHOQOL-BREF physical domain and the presence of sequelae (p=0.006), affective-cognitive inconsistency (p=0.009), and being the primary caregiver (p=0.002). The psychological domain showed an association with the presence of sequelae (p=0.006), the environment domain with the presence of diseases (p<0.001) and being the primary caregiver (p=0.033), and the social relations domain with the presence of diseases (p<0.001). CONCLUSION: The presence of sequelae or diseases, affective-cognitive inconsistency, and being the primary caregiver were related to decreases in their QOL.

Quality of life and associated factors for caregivers of functionally impaired elderly people / Quality of life and associated factors of caregivers for the elderly with impaired functional capacity

BACKGROUND: Lifestyle changes, factors inherent to the caregiver's family and social environment, and physical and mental aspects determine a caregiver's quality of life (QOL). OBJECTIVE: To evaluate the QOL and associated factors for caregivers of functionally impaired elderly people. METHOD: This was a cross-sectional study that analyzed data from a home-based care survey. The study sites were the homes of elderly people enrolled in Family Health Centers (Unidades de Saúde da Família) in Jequié, Bahia, Brazil. The sample consisted of 40 caregivers of functionally impaired elderly people. The instrument consisted of demographic data, health status, the Perceived Family Support Inventory, the Social Support Satisfaction Scale, and the World Health Organization Quality of Life Assessment (WHOQOL-BREF). The data were analyzed using a descriptive analysis and the chi-squared test at a 5% significance level, performed in SPSS version 15.0. RESULTS: An association was found between the WHOQOL-BREF physical domain and the presence of sequelae (p=0.006), affective-cognitive inconsistency (p=0.009), and being the primary caregiver (p=0.002). The psychological domain showed an association with the presence of sequelae (p=0.006), the environment domain with the presence of diseases (p<0.001) and being the primary caregiver (p=0.033), and the social relations domain with the presence of diseases (p<0.001). CONCLUSION: The presence of sequelae or diseases, affective-cognitive inconsistency, and being the primary caregiver were related to decreases in their QOL. .

CONTEXTUALIZAÇÃO: O rearranjo no modo de viver, acompanhado de fatores inerentes ao ambiente familiar e social do cuidador, além de aspectos físicos e mentais, irão se associar e serão fundamentais para a boa qualidade de vida (QV) da pessoa que cuida. OBJETIVO: Avaliar a QV e os fatores associados em cuidadores de idosos com comprometimento da capacidade funcional. MÉTODO: Trata-se de um estudo seccional que analisou dados de uma pesquisa de intervenção de base domiciliar. O local de estudo foram as residências dos idosos cadastrados em Unidades de Saúde da Família, no município de Jequié, BA, Brasil. A amostra foi constituída por 40 cuidadores de idosos com comprometimento da capacidade funcional. O instrumento foi constituído de informações sociodemográficas, condições de saúde, Inventário de Percepção do Suporte Familiar, Escala de Satisfação do Suporte Social e o WHOQOL-BREF. Os dados foram analisados no Programa Estatístico SPSS, versão 15.0, sendo realizada análise descritiva e aplicação do qui-quadrado de Pearson, com um nível de significância de 5%. RESULTADOS: Verificou-se associação entre o Domínio Físico do WHOQOL-BREF e as variáveis do estudo: presença de sequelas (p-valor=0,006), afetividade-consistência (p-valor=0,009) e ser cuidador principal (p-valor=0,002). O Domínio Psicológico apresentou associação com a presença de sequelas (p-valor=0,006) e o Domínio Meio Ambiente, com presença de doenças (p-valor<0,001) e ser o cuidador principal (p-valor=0,033) e o Domínio Relações Sociais com presença de doença (p-valor<0,001). CONCLUSÃO: Observou-se ...