ABSTRACT
BACKGROUND: Cisplatin (Cis-Pt) is a widely used anticancer drug but its therapeutic efficiency is limited by hemato-, cardio-, hepato-, nephro- and neurotoxicity. Complexation of Cis-Pt with C60 fullerene nanoparticle will allow to enhance the antitumor activity of the drug and to reduce its side toxic effects. AIM: To estimate the antitumor effects of С60-Cis-Pt nanocomplex in Lewis lung carcinoma (LLC) and analyze hematological toxicity in tumor-bearing mice. MATERIALS AND METHODS: Complexation of C60 fullerene and Cis-Pt molecule was studied by computer simulation. С60-Cis-Pt nanocomplex was i.p. injected to LLC-bearing mice in a total dose of 7.5 mg/kg (C60:Cis-Pt as 3.75:3.75 mg/kg). The survival of tumor-bearing mice and the relative reduction of tumor weight was recorded. Blood indices were determined using the Particle Counter PCE-210 automatic hematology analyzer. RESULTS: Computer simulation demonstrated the formation of С60-Cis-Pt nanocomplex in physiological medium and its stability due to the hydrophobic interactions. Treatment with C60-Cis-Pt nanocomplex increased survival time of LLC-bearing mice by 32%, normalized hemoglobin content (up to 100 g/l), erythrocyte and platelet count as compared to the untreated LLC-bearing mice. Tumor weight decreased by 35.5%; the mitotic index of tumor cells decreased by 78%, and apoptotic index increased by 75%. The revealed effects of the C60-Cis-Pt nanocomplex were more pronounced than the effects of Cis-Pt or C60 fullerene alone in equivalent dose. CONCLUSION: Treatment with C60-Cis-Pt nanocomplex prolonged the survival of LLC-bearing mice and reduced anemia in LLC-bearing mice.
Subject(s)
Antineoplastic Agents/therapeutic use , Carcinoma, Lewis Lung/drug therapy , Cisplatin/therapeutic use , Computer Simulation , Fullerenes/therapeutic use , Animals , Carcinoma, Lewis Lung/pathology , Cell Line, Tumor , Cisplatin/chemistry , Fullerenes/chemistry , Male , Mice , Mice, Inbred C57BL , Nanoparticles/chemistryABSTRACT
UNLABELLED: It is proposed to use the novel paradigm of treating cancer with hyperthermic therapy using multi-walled carbon nanotubes (MWCNT) stimulated with near infrared (NIR) irradiation. AIM: To establish the capacity of MWCNT stimulated with NIR irradiation to destroy Erlich ascitic carcinoma (EAC) cells. MATERIALS AND METHODS: EAC cells suspension was irradiated with NIR heating lamp with a wavelength of 0.78-1.40 mm and power density of 3.5 W/cm2 over 1.5 min in the presence of MWCNT (0.1 mg/ml). The changes in the temperature of suspension with the NIR exposure time was measured using the differential cooper-constantan thermocouple. The viability of EAC cells was evaluated by trypan blue staining. RESULTS: The death of 95.2% of EAC cells in the presence of MWCNT was observed after 1.5 min of NIR light irradiation: thermal ablation temperature was approximately 50 degrees C. CONCLUSIONS: It was demonstrated that addition of MWCNT to EAC cell suspension results in the photo-ablative destruction of cells exposed to short time NIR irradiation.
