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Preparation of Mouse Embryonic Fibroblasts as Feeders for iPSC Generation and Maintenance.
Brehm, Jennifer L; Miles, Molly M; Remondini, Katie S; Ludwig, Tenneille E.
Methods Mol Biol ; 2239: 213-234, 2021.
Article in English | MEDLINE | ID: mdl-33226622

ABSTRACT

Mouse embryonic fibroblasts (MEFs) can be used in co-culture to support generation of induced pluripotent stem cells (iPSCs) and the normal growth and proliferation of human pluripotent stem cells (hPSCs). Here, we describe the necessary steps to derive, expand, harvest, inactivate, plate, and use MEFs as feeders for iPSC generation and maintenance.


Subject(s)
Cell Culture Techniques/methods , Embryo, Mammalian/cytology , Feeder Cells/radiation effects , Fibroblasts/radiation effects , Animals , Cells, Cultured , Coculture Techniques/methods , Cryopreservation , Feeder Cells/cytology , Feeder Cells/physiology , Fibroblasts/cytology , Fibroblasts/physiology , Induced Pluripotent Stem Cells/physiology , Mice
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