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Synthesis, cloning and expression in Escherichia coli of the gene coding for the trypsin inhibitor from Cucurbita pepo.

Rempoøa, B; Wilusz, T; Markiewicz, W; Fikus, M.

Acta Biochim Pol ; 42(1): 109-14, 1995.

Article in English | MEDLINE | ID: mdl-7653151

ABSTRACT

A chemically synthesized gene coding for the serine proteinase inhibitor CPTI II was cloned in E. coli and its expression was investigated in cytoplasmic and secretion systems. Under all conditions investigated the biologically active form of the inhibitor was found only in the latter system, although the yield was rather low.

Subject(s)

Escherichia coli/metabolism , Plant Proteins/biosynthesis , Serine Proteinase Inhibitors/biosynthesis , Trypsin Inhibitors/biosynthesis , Vegetables/genetics , Amino Acid Sequence , Bacterial Outer Membrane Proteins/genetics , Base Sequence , Cloning, Molecular , Escherichia coli/genetics , Genes, Synthetic , Genetic Vectors , Isopropyl Thiogalactoside/pharmacology , Molecular Sequence Data , Plant Proteins/genetics , Protein Sorting Signals , Restriction Mapping , Serine Proteinase Inhibitors/genetics , Temperature , Time Factors , Trypsin/metabolism , Trypsin Inhibitors/genetics

ABSTRACT

Subject(s)

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