ABSTRACT
The antidiabetic effect of the total flavonoids fraction from leaves of Eriobotrya japonica (EJF) was evaluated through normal and streptozotocin-induced diabetic mice with graded oral doses of 150, 300, 450 mg/kg for 7 days or 14 days. The result showed that the dose of 300 mg/kg and 450 mg/kg resulted significant hypoglycemic effect on normal mice, the dose of 300 mg/kg induced significant decrease in plasma glucose concentration (PGC), glycosylated serum protein (GSP), total cholesterol (TC) and triglyceride (TG), and significant increase in superoxide dismutase (SOD) activity and serum insulin level in streptozotocin-diabetic mice. These results suggested that EJF has hypoglycemic potential.
Subject(s)
Diabetes Mellitus, Experimental/drug therapy , Eriobotrya/chemistry , Flavonoids/therapeutic use , Hypoglycemic Agents/analysis , Phytotherapy , Animals , Blood Glucose/drug effects , Blood Proteins/metabolism , Chromatography, High Pressure Liquid , Flavonoids/isolation & purification , Glycoproteins/metabolism , Insulin/blood , Lipids/blood , Male , Mice , Mice, Inbred ICR , Plant Extracts/chemistry , Plant Extracts/pharmacology , Plant Extracts/therapeutic use , Plant Leaves/chemistry , Spectrometry, Mass, Electrospray Ionization , Superoxide Dismutase/blood , Glycated Serum ProteinsABSTRACT
For seeking the good natural material to develop new agent to treat diabetes, the total triterpene acid (TTA) fraction extracted from Folium Eriobotryae [leaves of Eriobotrya japonica (Thunb.) Lindl.] was evaluated for its hypoglycemic and hypolipidemic potential through normal, alloxan and streptozotocin-induced diabetic mice administered with graded oral doses (100, 200, 300 mg/(kg day)) for 7 or 14 days. The results showed that a dose of 300 mg/kg of TTA is the most effective dose to cause significant (p<0.01) hypoglycemic and/or hypolipidemic effects on normal, alloxan and streptozotocin-induced diabetic mice. This dose also significantly (p<0.01) lowered the glycosylated serum protein (GSP), total cholesterol (TC) and triglyceride (TG) level in severely diabetic mice. Furthermore, TTA increased the superoxide dismutase activity (SOD) and the serum insulin level of diabetic mice. These evidences indicated that the total triperpene acid fraction from Folium Eriobotryae has a high anti-diabetic potential along with a good hypolipidemic profile.
Subject(s)
Diabetes Mellitus, Experimental/drug therapy , Eriobotrya/chemistry , Hypoglycemic Agents/therapeutic use , Hypolipidemic Agents/therapeutic use , Phytotherapy , Plant Extracts/therapeutic use , Triterpenes/therapeutic use , Animals , Blood Glucose , Blood Proteins , Cholesterol/blood , Dose-Response Relationship, Drug , Glycoproteins/blood , Hypoglycemic Agents/pharmacology , Hypolipidemic Agents/pharmacology , Insulin/blood , Male , Mice , Plant Extracts/pharmacology , Plant Leaves , Superoxide Dismutase/blood , Triglycerides/blood , Triterpenes/pharmacology , Glycated Serum ProteinsABSTRACT
Sesquiterpene glycoside, nerolidol-3-O-alpha-l-rhamnopyranosyl(1-->4)-alpha-l-rhamnopyranosyl(1-->2)-[alpha-l-rhamnopyranosyl(1-->6)]-beta-d-glucopyranoside was isolated from dried leaves of loquat [Eriobotrya japonica (Thunb.) Lindl., Rosaceae]. Hypoglycemic effects of this natural product were assessed in normal and alloxan-diabetic mice model. Animals received orally administration of the sesquiterpene glycoside in dose of 25 and 75 mg/kg. The anti-hyperglycemic effect was compared with gliclazide's. The dose of 25 and 75 mg/kg both exerted a significant (p<0.05) hypoglycemic effect in alloxan-diabetic mice throughout the test and a slight effect in normal mice.
