Impact of sleep status on lung adenocarcinoma risk: a prospective cohort study.

OBJECTIVE: The association between sleep status and lung adenocarcinoma risk was analyzed using long-term follow-up data from 60,443 patients over the period 2016-2022 to provide a reference for exploring the association between sleep status and lung adenocarcinoma development. PATIENTS AND METHODS: Based on long-term follow-up data, a total of 60,443 people were included. Sleep data collected for the study included insomnia symptoms, lunch break habits, and sleep duration. A sleep score (0-3) was constructed based on difficulty falling asleep, premature awakening and sleep duration. Proportional risk regression models were used to analyze the association between each sleep factor, sleep score and lung cancer risk. RESULTS: The study population was followed up for 9.9 ± 4.8 years and a total of 307 cases of lung adenocarcinoma were first recorded during the follow-up period. After controlling for potential confounders, the risk ratios (HR) for lung adenocarcinoma in those with difficulties going asleep or waking up too early were 1.12 (95% CI: 1.02-1.14) and 1.07 (95% CI: 1.01-1.11), respectively, compared to those without symptoms of insomnia. The HR for lung adenocarcinoma in those with less than 7 h of sleep [HR = 1.17 (95% CI: 1.05-1.21)] was compared to those with ≥ 7 h of sleep per day. Compared to those with a sleep score of 3 (highest quality sleep), those with a sleep score of 2, 1 and 0 corresponded to HR of 1.06 (95% CI: 1.01-1.12), 1.11 (95% CI: 1.09-1.18) and 1.15 (95% CI: 1.01-1.32) respectively. CONCLUSIONS: Patients who suffer from insomnia or have a short sleep schedule are at increased risk of developing lung cell cancer. Sleep has an important impact on health and improving sleep conditions can reduce the incidence of lung cancer.

Effect of dietary energy restriction and subsequent compensatory feeding on testicular transcriptome in developing rams.

Nutritional intake and reproductive allocation are strongly associated and dietary energy restriction (ER) or surpluses can affect reproductive capacity. The objective of this study was to investigate the effect of energy levels on sheep testicular development. Three-month old Hu sheep were assigned to four groups, and fed diets containing different levels of energy (Control, maintenance energy; ER1, 85% maintenance energy; ER2, 70% maintenance energy; ER3, 55% maintenance energy). Two months later, half the sheep in each group were euthanized, whereas the remaining sheep were euthanized after a further 3 months feeding on a compensatory energy diet. The testicular weight and reproductive hormone levels of the Hu sheep were investigated. Differences in the testes of ER3 and control group sheep were investigated at the transcriptional level using high-throughput sequencing. The results showed that the testicular weights had decreased in the energy-restricted rams compared with the controls, and that the testosterone concentration in ER3 group rams was significantly lower than that in other compared groups (P < 0.05). After the period of compensatory feeding, however, ER3 sheep testicular weight and testosterone concentrations were similar to those of the control group sheep. In addition, the RNA sequencing results revealed that 81 genes were upregulated and 180 genes were downregulated in the ER3 group compared with the control group. Moreover, based on the enriched steroidogenesis, meiosis and kinases pathways, a number of candidate genes potentially involved in the regulation of testicular development or reproduction of Hu sheep, including CYP11A1, ALDH3B1, FDFT1, WNT2, PGR and INSR, were screened. Quantitative real-time polymerase chain reaction analysis results correlated well with the sequencing data. Taken together, this study provides a first insight into the development of the testis with dietary energy restriction in sheep and shows that these changes are associated with alterations in transcriptomic. The sheep testis mRNA database were extended in this study will provides novel candidate regulators for future genetic and molecular studies on sheep testicular development associated with energy restriction, which will contribute to improving the reproductive performance of sheep.

[Effect of vitamin C (V.C) on thermal sensitivity of Lewis tumor].

In this paper, the effect of V.C on thermal sensitivity of the tumor and normal tissues was studied. Average peak reaction and RD50 (50% reaction dosage) were used to evaluate the effect of V.C on thermal sensitivity of the normal tissue. Tumor cure rate and TCD50 (50% tumor cure dosage) were used to evaluate the effect of V.C on that of the tumor tissue. In all mice injected by V.C locally, the average peak reaction markedly increased, RD50 decreased by 1-2 fold; the tumor cure rate was obviously elevated, TCD50 was significantly reduced except those treated by 20' hyperthermia. TGF (therapeutic gain factor) was 1.76. These results indicate that V.C can increase the thermal sensitivity of tumor and normal tissues but the effect on tumor tissue is selective.

[Radiotherapy of chordoma--a report of 10 patients].

A consecutive series of 10 patients with chordoma was treated in our hospital from 1956 to 1982. Six were male and four were female. The age ranged 19 approximately 52 years. Eight lesions occurred in the base of sphenoid and two in the sacrum. All were treated by postoperative radiotherapy with a medium dose of 4,500 approximately 6,000 rad. Recurrent patients were given a hyperfractionated radiotherapy (100 rad, 4/D, at intervals of 3 hours, total dose 2000 rad). The 5 and 10 year survival rates were 75% and 50%. No complications were found after radiation. In this paper, literature on chordoma and radiotherapy for chordoma are reviewed. An optimal schedule is proposed: operation be the choice of treatment, which be as thorough as possible; two months after the operation, radiotherapy be given at a suitable dose of 5000 approximately 6000 rad; the recurrent lesions be treated by hyperfractionated radiotherapy.