Eâ ¡B Mutation Reduces the Pathogenicity of Listeria monocytogenes by Negatively Regulating Biofilm Formation Ability, Infective Capacity, and Virulence Gene Expression.

To explore the role of the membrane permease ⅡB (EⅡB) gene of Listeria pathogenicity island 4 (LIPI-4) in the virulence of Listeria monocytogenes, both an EⅡB deletion strain (∆EⅡB) and a complemented strain were constructed. In vitro experiments demonstrated that EⅡB deletion affected the biofilm formation ability of the wild-type strain (Lm928). Moreover, this deletion decreased the intracellular proliferation abilities of L. monocytogenes. Mice infected with ∆EⅡB survived longer and experienced less weight loss on days 1, 2, and 3 post-infection. The bacterial load in the liver tissue of ∆EⅡB-infected mice was significantly reduced, and a considerable decrease in the blood levels of inflammatory cytokines IL-ß, IL-6, IL-10, and TNF-α were observed. Following EⅡB deletion, 65% (13/20) of genes were downregulated, 25% (5/20) were upregulated, and 10% (2/20) showed no change. These findings suggest that EⅡB deletion may reduce both the in vivo and in vitro virulence levels as well as the biofilm formation ability of Lm928 by downregulating the transcription levels of genes associated with virulence and biofilm formation. These findings provide a foundation for further examining the pathogenic mechanisms of LIPI-4 and EⅡB in L. monocytogenes.

Application of physical training in conjunction with rehabilitation in male soccer injury / Aplicação de treinamento físico em conjunto à reabilitação de lesões no futebol masculino / Aplicación de entrenamiento físico junto con la rehabilitación de lesiones en el fútbol masculino

ABSTRACT Introduction: Soccer is one of the sports with the highest incidence of injuries, generated both by the high performance required on the field and by physical conflicts between players. A fast rehabilitation is essential for the player's performance. It has been empirically observed that an early recovery in patients' rehabilitation is associated with physical training compared to players who received only the traditional rehabilitation. Objective: Verify the physical training influence on rehabilitation sports injuries in male soccer players. Method: 180 cases of male adolescents with sports injuries admitted to a Taiyuan hospital were selected. A division into two groups was randomly computerized to avoid statistical differences in the intensity of the injuries. The control group (14.3±2.45 years old) was treated with the standard protocol, while the experimental group (15.3±2.95 years old) received an intervention with physical training. Interviews and questionnaires were conducted involving analysis of time and severity of the injury, laterality, and location. The control group received treatment based on this information. The recovery rate and intervention satisfaction rate were collected. SPSS22.0 Statistical Software was used for student's t-test and chi-square test. Results: Treatment efficiency was 82 (91.11%) in the control group versus 88 (97.78%) in the experimental group. The degree of dissatisfaction was 1 (2.11%) versus 8 (10%). The perceived overall satisfaction was 80 (89%) versus 87 (96.67%), (p <0.05). Conclusion: Rehabilitation associated with physical training intervention improved satisfaction and treatment efficiency. Evidence Level II; Therapeutic Studies - Investigating the results.

RESUMO Introdução: O futebol é um dos esportes com maior incidência de lesões, geradas tanto pelo alto desempenho exigido em campo quanto por conflitos físicos entre jogadores. Uma breve reabilitação é essencial para o rendimento do jogador. Percebe-se, de forma empírica, uma alta precoce em pacientes submetidos ao treinamento físico associado, quando comparados aos jogadores destinados à reabilitação tradicional. Objetivo: Verificar a influência do treinamento físico na reabilitação de lesões esportivas nos jogadores de futebol masculino. Método: Foram selecionados 180 casos de adolescentes masculinos com lesões esportivas internados neste hospital. A divisão em dois grupos foi efetuada aleatoriamente de forma computadorizada afim de evitar diferenças estatísticas na intensidade das lesões. O grupo controle (idade média 14,3±2,45 anos) foi tratado com o protocolo padrão enquanto ao experimental (idade média 15,3±2,95 anos) foi adicionada uma intervenção com treinamento físico. Foram efetuadas entrevistas e questionários envolvendo análise de tempo e gravidade da lesão, lateralidade e localização. O grupo controle recebeu o tratamento norteado nessas informações. Foram coletados a taxa de recuperação e a taxa de satisfação de intervenção. Foi utilizado o Software Estatístico SPSS22.0 para os testes t de student e qui-quadrado. Resultados: A eficiência do tratamento foi de 82 (91,11%) no grupo controle contra 88 (97,78%) no grupo experimental. O grau de insatisfação foi de 1 (2,11%) contra 8 (10%). A percepção de satisfação total foi de 80 (89%) contra 87 (96,67%), (p <0,05). Conclusão: A reabilitação associada à intervenção de treinamento físico demonstrou melhorar a taxa de satisfação e a eficiência do tratamento. Nível de evidência II; Estudos Terapêuticos - Investigação de Resultados.

RESUMEN Introducción: El fútbol es uno de los deportes con mayor incidencia de lesiones, generadas tanto por el alto rendimiento requerido en el campo como por los conflictos físicos entre los jugadores. Una pronta rehabilitación es esencial para el rendimiento del jugador. Se ha observado empíricamente que una recuperación temprana en la rehabilitación de los pacientes se asocia con la adición del entrenamiento físico en comparación con los jugadores que sólo recibieron la rehabilitación tradicional. Objetivo: Verificar la influencia del entrenamiento físico en la rehabilitación de lesiones deportivas en jugadores de fútbol masculinos. Métodos: Se seleccionaron 180 casos de adolescentes masculinos con lesiones deportivas ingresados en un hospital de Taiyuan. Se realizó una división en dos grupos de forma aleatoria para evitar diferencias estadísticas en la intensidad de las lesiones. El grupo de control (14,3±2,45 años) fue tratado con el protocolo estándar, mientras que el grupo experimental (15,3±2,95 años) recibió una intervención con entrenamiento físico. Se realizaron entrevistas y cuestionarios que incluían el análisis del tiempo y la gravedad de la lesión, la lateralidad y la localización. El grupo de control recibió un tratamiento basado en esta información. Se recogió el índice de recuperación y el índice de satisfacción de la intervención. Se utilizó el software estadístico SPSS22.0 para la prueba t de student y la prueba chi-cuadrado. Resultados: La eficacia del tratamiento fue de 82 (91,11%) en el grupo de control frente a 88 (97,78%) en el grupo experimental. El grado de insatisfacción fue de 1 (2,11%) frente a 8 (10%). La satisfacción global percibida fue de 80 (89%) frente a 87 (96,67%), (p <0,05). Conclusión: La rehabilitación asociada a la intervención de entrenamiento físico mejoró la satisfacción y la eficacia del tratamiento. Nivel de evidencia II; Estudios terapéuticos - Investigación de resultados.

Chinese patent medicine Bailing capsule for treating lupus nephritis: A protocol for systematic review and meta-analysis.

BACKGROUND: Treatment of lupus nephritis (LN) remains challenging. LN remains the primary source of morbidity and mortality in patients with systemic lupus erythematosus. Chinese patent medicine Bailing capsule is commonly used for the treatment of LN. However, there is no consensus on the difference in clinical efficacy compared with routine treatment. Therefore, we plan to perform a systematic review and meta-analysis to review the clinical efficacy and safety of Bailing capsule for LN. METHODS: Cochrane Central Register of Controlled Trials, PubMed, EMBASE, Chinese National Knowledge Infrastructure, Chinese Biomedical Database, Wanfang database, and VIP information database will be searched from their inception until January 2019. The meta-analysis will be conducted with Review Manager 5.3 software to systematically review the clinical efficacy and safety of Bailing capsule for LN. The primary outcome will include clinical effective rate, and the secondary outcomes will include Systemic Lupus Erythematosus Disease Activity Index, serum creatinine, 24-hour urine protein quantity, complement 3, and adverse effects. RESULTS: This analysis will provide useful information about clinical efficacy and safety of Bailing capsule for LN. CONCLUSIONS: Our study will generate strong evidence of Bailing capsule for patients with LN and provide suggestions for clinical practice.