ABSTRACT
OBJECTIVE: To establish initial processing technology of Corydalis yanhusuo. METHODS: Investigated the effect of the factors such as slice method, dry method, drying temperature on the content of water-extract, ethanol-extract, effective component in Corydalis yanhusuo pieces. Compared the quality with that of the traditional initial processing samples. RESULTS: The best initial process method was: cut fresh Corydalis yanhusuo into 4 - 5 mm thick slices, dry at 70 - 80 degrees C or microwave dry. CONCLUSION: The study provides theoretical base for modifying the initial processing.
Subject(s)
Alkaloids/analysis , Berberine Alkaloids/analysis , Corydalis/chemistry , Desiccation/methods , Plants, Medicinal/chemistry , Chromatography, High Pressure Liquid/methods , Ethanol , Microwaves , Plant Extracts/analysis , Plant Extracts/chemistry , Quality Control , Reproducibility of Results , Rhizome/chemistry , Technology, Pharmaceutical/methodsABSTRACT
OBJECTIVE: To choose the best harvest time and initial processing method of Rhizoma Corydalis. METHODS: Taking the content of tetrahydropalmatine and dry rates as major indices, different samples from different harvest time and different processing methods were investigated by HPLC. The C18 column (250 mm x 4.6 mm, 5 microm) was used with mobile phase of methanol-0.1% phosphoric acid solution (pH = 6.0, adjusted with triethylamine) (58: 42). The mobile phase flow rate was 1.0 mL/min. The detection wavelength was 280 nm. RESULTS: The contents of tetrahydropalmatine were similar although the harvest time was different. The dry rate on May 21 was higher than the others. The content of tetrahydropalmatine was the highest by steaming and reached 0.11%. CONCLUSION: The best harvest time for Rhizoma Corydalis is around 10 days after the above-ground plant wilted, and the best initial processing method for Rhizoma Corydalis is steaming.
Subject(s)
Berberine Alkaloids/analysis , Corydalis/chemistry , Drugs, Chinese Herbal/chemistry , Plants, Medicinal/chemistry , Technology, Pharmaceutical/methods , Berberine Alkaloids/isolation & purification , Chromatography, High Pressure Liquid/methods , Corydalis/growth & development , Drugs, Chinese Herbal/isolation & purification , Hot Temperature , Plants, Medicinal/growth & development , Reproducibility of Results , Rhizome/chemistry , Seasons , SteamABSTRACT
The experiment of cutting propagation of Tripterygium wilfordii Hook. f. was carried out. It showed a better result when stems were instantly planted after being moistened or soaked by 500 - 1000 ppm IBA or NAA. A remarkably higher survival rate was achieved while plasticfilm and shade were accompanied. September and October or rainy June are prime moments for operation. It' s recommendable to choose those newly corking twigs for cuttage.
