Long-term grazing improved soil chemical properties and benefited community traits under climatic influence in an alpine typical steppe.

Grazing and climate change both contribute to diversity loss and productivity fluctuations. Sensitive climate conditions and long-term grazing activities have a profound influence on community change, particularly in high-altitude mountain grassland ecosystems. However, knowledge about the role of long-term continuous grazing management on diversity, productivity and the regulation mechanisms in fragile grassland ecosystems is still rudimentary. We conducted a long-term grazing experiment on an alpine typical steppe in the Qilian Mountains to assess effects of grazing intensity on soil, diversity, productivity and the regulation mechanisms. Plants and soil were sampled along grazing gradients at different distances from the pasture entrance (0, 0.3, 0.6, 0.9, 1.2 and 1.5 km) under the non-growing (WP) and the growing season grazing pasture (SAP). The results revealed that community diversity and biomass did not change significantly on a time scale, while the concentration of soil organic carbon and total phosphorus increased significantly. Heavy grazing (0-0.3 km) decreased community diversity and biomass. Grazing increased soil chemical properties in heavy grazed areas of WP, while the opposite was recorded in SAP. Soil chemical properties explained the largest variances in community diversity and community biomass. The prediction model indicates that grazing in WP mainly affects community diversity through soil chemical properties, and promotes a positive correlation between community diversity and community biomass; in SAP, the direct effect of grazing gradients on community diversity and biomass is the main pathway, but not eliminating the single positive relationship between diversity and biomass, which means that diversity can still be used as a potential resource to promote productivity improvement. Therefore, we should focus on the regulation of soil chemical properties in WP, such as the health and quality of soil, strengthening its ability to store water, sequester carbon and increase nutrients; focus on the management of livestock in SAP, including providing fertilizer and sowing to increase diversity and production in heavily grazed regions and reducing grazing pressure through regional rotational grazing. Ultimately, we call for strengthening the stability and sustainability of ecosystems through targeted and active human intervention in ecologically sensitive areas to cope with future grazing pressures and climate disturbances.

A Novel High-Sensitivity MEMS Pressure Sensor for Rock Mass Stress Sensing.

This paper proposes a novel high-sensitivity micro-electromechanical system (MEMS) piezoresistive pressure sensor that can be used for rock mass stress monitoring. The entire sensor consists of a cross, dual-cavity, and all-silicon bulk-type (CCSB) structure. Firstly, the theoretical analysis is carried out, and the relationship between the structural parameters of the sensor and the stress is analyzed by finite element simulation and curve-fitting prediction, and then the optimal structural parameters are also analyzed. The simulation results indicate that the sensor with the CCSB structure proposed in this article obtained a high sensitivity of 87.74 µV/V/MPA and a low nonlinearity error of 0.28% full-scale span (FSS) within the pressure range of 0-200 MPa. Compared with All-Si Bulk, grooved All-Si Bulk, Si-Glass Bulk, silicon diaphragm, resistance strain gauge, and Fiber Bragg grating structure pressure sensors, the designed sensor has a significant improvement in sensitivity and nonlinearity error. It can be used as a new sensor for rock disaster (such as collapse) monitoring and forecasting.

Development of biomaterials based on plasticized polylactic acid and tea polyphenols for active-packaging application.

Bioactive-packaging films based on polylactic acid (PLA), acetyl tributyl citrate (ATBC), and tea polyphenol (TP) were prepared by melt blending. Results of mechanical-property test revealed that adding ATBC and TP can significantly improve mechanical properties of PLA. The shift of CO to lower wavelengths in FTIR and the morphology of the films in SEM indicated physical or chemical interactions in the PLA/ATBC/TP films. The antioxidant, and antibacterial activities of the PLA/ATBC films increased dramatically (P<0.05) with increased TP amount. The antioxidant activity of the films with 1 % TP was equivalent to that of 300 mg/L l-ascorbic acid, whereas PLA/ATBC/TP films with 0.5 % and 1 % TP concentration were effective in inhibiting Staphylococcus aureus and Escherichia coli within almost 5 h (P<0.05). The PLA films changed from transparent to opaque and from yellow to red after combining with ATBC or TP, respectively. The overall migration of the films in 3 % acetic acid and 10 % ethanol did not exceed the overall migration limit. All these findings indicated potential of the PLA/ATBC/TP films in active-packaging application.

Grazing Intensity Alters Leaf and Spike Photosynthesis, Transpiration, and Related Parameters of Three Grass Species on an Alpine Steppe in the Qilian Mountains.

The effect of grazing on leaf photosynthesis has been extensively studied. However, the influence of grazing on photosynthesis in other green tissues, especially spike, has remained poorly understood. This study investigated the impact of different grazing intensities (light grazing (LG), medium grazing (MG), and heavy grazing (HG)) on leaf and spike photosynthesis parameters and photosynthetic pigments of three grass species (Stipa purpurea, Achnatherum inebrians, and Leymus secalinus) on an alpine steppe in the Qilian Mountains. Grazing promoted leaf photosynthesis rate in S. purpurea and L. secalinus but reduced it in A. inebrians. Conversely, spike photosynthesis rate decreased in S. purpurea and L. secalinus under intense grazing, while there was no significant difference in spike photosynthesis rate in A. inebrians. The leaf and spike net photosynthetic rate (Pn) and transpiration rate (Tr) in S. purpurea were the greatest among the three species, while their organ temperatures were the lowest. On the other hand, grazing stimulated leaf chlorophyll biosynthesis in S. purpurea and L. secalinus but accelerated leaf chlorophyll degradation in A. inebrians. Furthermore, spike chlorophyll biosynthesis was inhibited in the three species under grazing, and only L. secalinus had the ability to recover from the impairment. Grazing had a positive effect on leaf photosynthesis parameters of S. purpurea and L. secalinus but a negative effect on those of A. inebrians. However, spike photosynthesis parameters were negatively influenced by grazing. Among the three species investigated, S. purpurea displayed the greatest ability for leaf and spike photosynthesis to withstand and acclimate to grazing stress. This study suggests that moderate grazing enhanced leaf photosynthetic capacity of S. purpurea and L. secalinus but reduced it in A. inebrians. However, spike photosynthetic capacity of three grass species decreased in response to grazing intensities.

Development of biobased multilayer films with improved compatibility between polylactic acid-chitosan as a function of transition coating of SiOx.

A SiOx coating prepared via plasma-enhanced chemical vapor deposition (PECVD) technique was used as a transition layer between polylactic acid (PLA) and chitosan (CS) to form a three-layer composite film of PLA/SiOx/CS. The effect of oxygen plasma irradiation time (0 s, 30s and 60s) on SiOx surface and PLA-CS interface was examined based on the contact angle, pull-off test and scanning electron microscope (SEM). The mechanical, barrier and antibacterial properties of PLA/SiOx/CS films were also investigated. The results showed that oxygen plasma irradiation improved the hydrophilicity of the SiOx surface and the adhesive strength between SiOx-CS of PLA/SiOx/CS films in a time-dependent manner. SEM examination revealed a gap between PLA/CS layers, but the interfacial separation among layers in PLA/SiOx/CS films disappeared as the transition layer of SiOx and oxygen plasma irradiation (60s) intensified. Notably, the oxygen barrier property and antibacterial activity of PLA/SiOx/CS films were dramatically enhanced. Additionally, moisture resistance was slightly decreased following the incorporation of the CS coating compared with the PLA film. The tensile strength of the composite film also increased with the number of layers, while elongation at break decreased. The prepared PLA/SiOx/CS films with multifunction are promising applied in food packaging as biobased materials.

Assessing the spatiotemporal variation in distribution, extent and NPP of terrestrial ecosystems in response to climate change from 1911 to 2000.

To assess the variation in distribution, extent, and NPP of global natural vegetation in response to climate change in the period 1911-2000 and to provide a feasible method for climate change research in regions where historical data is difficult to obtain. In this research, variations in spatiotemporal distributions of global potential natural vegetation (PNV) from 1911 to 2000 were analyzed with the comprehensive sequential classification system (CSCS) and net primary production (NPP) of different ecosystems was evaluated with the synthetic model to determine the effect of climate change on the terrestrial ecosystems. The results showed that consistently rising global temperature and altered precipitation patterns had exerted strong influence on spatiotemporal distribution and productivities of terrestrial ecosystems, especially in the mid/high latitudes. Ecosystems in temperate zones expanded and desert area decreased as a consequence of climate variations. The vegetation that decreased the most was cold desert (18.79%), while the maximum increase (10.31%) was recorded in savanna. Additionally, the area of tundra and alpine steppe reduced significantly (5.43%) and were forced northward due to significant ascending temperature in the northern hemisphere. The global terrestrial ecosystems productivities increased by 2.09%, most of which was attributed to savanna (6.04%), tropical forest (0.99%), and temperate forest (5.49%). Most NPP losses were found in cold desert (27.33%). NPP increases displayed a latitudinal distribution. The NPP of tropical zones amounted to more than a half of total NPP, with an estimated increase of 1.32%. The increase in northern temperate zone was the second highest with 3.55%. Global NPP showed a significant positive correlation with mean annual precipitation in comparison with mean annual temperature and biological temperature. In general, effects of climate change on terrestrial ecosystems were deep and profound in 1911-2000, especially in the latter half of the period.

Isolation and characterization of GoRAV, a novel gene encoding a RAV-type protein in Galegae orientalis.

RAV is a unique transcription factor in higher plants with AP2 and B3-like two distinct DNA-binding domains, but its roles in plant growth and development remains unknown. We have isolated a novel RAV family gene from Galegae orientalis, called GoRAV, which responds to cold induction. Sequence alignment showed that it shares high identity with other RAV family members in AP2 and B3 domain. Transient expression analysis using onion epidermal cells indicated that GoRAV protein is localized in the nucleus. Semi-quantitative RT-PCR (S-Q RT-PCR) analysis indicated that GoRAV is induced by cold, dehydration, high-salinity and abscisic acid, with the strongest induction in G. orientalis leaves during the early response to abiotic elicitors. GoRAV is more abundant in leaf than in stem, but is not expressed in root. This work adds a new member to the RAV family.

[Food nourishment utilization efficiency of different month aged sheep].

Studies on the food nourishment utilization efficiency of different month aged sheep showed that under same food nourishment condition, the ingested nourishment, weight increase, and nourishment depletion per unit weight increase of sheep were increased with the ingestion of dry matter, and the protein content of 34 months old sheep was significantly higher than that of 22 months old sheep (P < 0.01), and the latter was significantly higher than that of 10 months old sheep (P < 0.01). There was a significant difference of utilization efficiency of the ingested nourishment between 34 months old and 22 months old sheep (P < 0.05) and between 10 months old and 22 months old sheep (P < 0.01). The weight increase was not significantly different (P > 0.05) between 34 months old and 22 months old sheep and between 10 months old and 22 months old sheep, but it was significantly different between 34 months old and 10 months old sheep (P < 0.05). The feed conversion rate among them was not different. All the results showed that 10-22 months old sheep had high production efficiency, and could produce high quality mutton, and hence, this section of age was the suitable age of sheep to be butchered.

[Landscape pattern and productivity characteristics of the oasis landscape ecosystem in Linze, Gansu, China].

Oasis landscape ecosystem is composed of 10 landscape elements, i.e., residence land, cultivated land, grassland, forestland, water area, water system, road, rocky desert, sandy desert, and gravel desert. Among the elements, cultivated land formed by human being production covers the most of the area, is most connected, and hence, is the matrix of the oasis landscape ecosystem. Residence land, grassland, forestland, water area, rocky desert, sandy desert, and gravel desert are patches. Residence land and forestland generate from human being production, while rocky desert, gravel desert and sandy desert are the remnant with the human being disturbance. Water region and grassland are the environmental resources remnant after natural disturbance. Water system and road are corridors. Cultivated land dominated in plant production should be utilized with more productive layers through developing animal production other than expanding used-area to maintain the landscape heterogeneity and diversity of the oasis landscape ecosystem. For remnant and environmental resource patches, it should be profitable in preserving and stabilizing landscape heterogeneity and diversity, exploiting the functions of water and soil conservation, tourism, windbreak and sand fixation. For landscape elements remnant only, it should be fruitful in avoiding degeneration of the landscape pattern to explore their preceding plant production with moderate plant production.

[Landscape ecological mechanism on system coupling of the meta-ecosystem consisted of mountain, desert and oasis in Hexi corridor, Gansu, China].

The fundament of system coupling is heterogeneity, and the basic prerequisite is that there are connective corridors in the same type between ecosystems. The landscape ecological mechanism of system coupling is the spatial difference of non-biotic environment and the heterogeneity caused by disturbances. The force or energy of system coupling is disturbances. From in the view of landscape ecology, system coupling is the merging process of different landscape elements between different landscape ecosystems followed by the process of character changing of landscape elements with scale changing. Based on the essence of disturbances, system coupling can be divided into two types as natural system coupling and artificial system coupling. Natural system coupling is the base of artificial system coupling, and hence, the enhance of eco-productivity of coupling system is based on the optimization of artificial system coupling.

[Research progress and trend on grassland agroecology].

The connotation, progress, research frontiers and developmental trend of grassland agroecology are discussed in this paper. The interface theory, structure and function, coupling and discordance, and health assessment of grassland agroecosystems were recognized as the four research frontiers of the discipline. There exist three primary interfaces in a grassland agroecosystem, i.e., vegetation-site, grassland-animal and production-management. Research into a series of the ecological processes that occurred at these interfaces is the key to revealing the features of the system behavior. There are four sections in a grassland agroecosystem, i.e., pre-plant, plant, animal and post-biotic sections. System coupling and discordance are the two important concepts to describe interactions among the production sections. System coupling among the sections can lead to system improvement by exerting the potential of system capacity. Health of an ecosystem is a reflection of its structure and function, and health assessment is a measurement of its orderliness and service value.

[Bio-geological temporal zonation and its agricultural significance].

Bio-geological temporal zonation can be defined as a subsistent spatial-temporal style that the organisms clip from a geographical zone to break through its limit, and extend their survival extent with the strategy of excluding the environmental period of time which is not suitable to live and intercepting it which is suitable. It is the consequence that organisms temporally adapt the heat distribution pattern caused by the rotation and revolution of the Earth. Bio-geological temporal zonation relies on spatial zone, and the zonation spectrum changes in different spatial zones and has different basal heat zone. It appears as spiral loop based on its own spatial zone, and implies temporally seasonal change of the heat. Similarly, bio-geological temporal zonation relies on the distribution process of water and heat under spatial and temporal scale. The component, range and hierarchy of zonation spectrum change with spatial and temporal scale, and organisms adapt this kind of change with different ecological strategy depended on different ecological strategy. Human being can tune up bio-geological temporal zonation only in small scale.