ABSTRACT
Divertor detachment offers a promising solution to the challenge of plasma-wall interactions for steady-state operation of fusion reactors. Here, we demonstrate the excellent compatibility of actively controlled full divertor detachment with a high-performance (ßN ~ 3, H98 ~ 1.5) core plasma, using high-ßp (poloidal beta, ßp > 2) scenario characterized by a sustained core internal transport barrier (ITB) and a modest edge transport barrier (ETB) in DIII-D tokamak. The high-ßp high-confinement scenario facilitates divertor detachment which, in turn, promotes the development of an even stronger ITB at large radius with a weaker ETB. This self-organized synergy between ITB and ETB, leads to a net gain in energy confinement, in contrast to the net confinement loss caused by divertor detachment in standard H-modes. These results show the potential of integrating excellent core plasma performance with an efficient divertor solution, an essential step towards steady-state operation of reactor-grade plasmas.
ABSTRACT
The article "HOTTIP regulates progression of endometrial cancer via activating PI3K/AKT pathway, by Q. Guan, Q. Zhang, C. Zhang, Q. Liu, Q.-L. Ren, published in Eur Rev Med Pharmacol Sci 2018; 22(12): 3727-3733-DOI: 10.26355/eurrev_201806_15252-PMID: 29949146" has been withdrawn from the authors. The Publisher apologizes for any inconvenience this may cause. https://www.europeanreview.org/article/15252.
ABSTRACT
OBJECTIVE: To investigate the possible role of HOTTIP in the pathogenesis of endometrial cancer (EC) and its underlying mechanism. PATIENTS AND METHODS: 76 EC tissues and 76 adjacent normal tissues were collected in this study. HOTTIP expression was detected by qRT-PCR (quantitative Real-Time Polymerase Chain Reaction), and its relationship with clinical prognosis of EC patients was then analyzed. The effect of in vitro HOTTIP on proliferation, cell cycle, apoptosis, colony formation, and migration was examined, respectively. Furthermore, the impact of HOTTIP on PI3K/AKT pathway was explored. RESULTS: HOTTIP was remarkably overexpressed in EC patients. The survival rate of EC patients with high expression of HOTTIP was lower than that of patients with low expression, whereas the pathological grade and tumor size in high expression group were markedly higher than those of low expression group. After upregulation of HOTTIP by lentivirus transfection, the proliferation, colony formation, and migration of EC cells showed a remarkable increase, whereas cell apoptosis was remarkably inhibited. In addition, high expression of HOTTIP promoted the EC development by activating PI3K/AKT pathway. CONCLUSIONS: Overexpressed HOTTIP promotes the development of endometrial cancer via activating PI3K/AKT pathway.
Subject(s)
Disease Progression , Endometrial Neoplasms/metabolism , Phosphatidylinositol 3-Kinases/metabolism , Proto-Oncogene Proteins c-akt/metabolism , RNA, Long Noncoding/biosynthesis , Signal Transduction/physiology , Adult , Apoptosis/physiology , Cell Proliferation/physiology , Endometrial Neoplasms/genetics , Endometrial Neoplasms/pathology , Female , Gene Expression Regulation, Neoplastic , Humans , Middle Aged , Phosphatidylinositol 3-Kinases/genetics , Proto-Oncogene Proteins c-akt/genetics , RNA, Long Noncoding/geneticsABSTRACT
A simple and direct reversed-phase high performance liquid chromatography (RP-HPLC) method with UV detection was developed and validated for the determination of mono- and di-D-α-tocopherol polyethylene glycol 1000 succinate (TPGS 1000) in TPGS mixture. Before the HPLC analysis, mono- and di-TPGS 1000 were separated by simulated moving bed (SMB) chromatography system and characterized by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). The mass spectrometric results confirmed that the molar mass distribution of TPGS prepared in our laboratory was very close to that of the product of Eastman Chemical Company with similar n¯ (average polymerization degree), M(n)¯ (number-average molecular weight) and M(w)¯ (weight-average molecular weight). The HPLC analysis was carried out on a C30 analytical column with mobile phases comprised of acetonitrile (A) and isopropanol (B) in gradient conditions. Validation of the analytical method was done on the following parameters: system suitability, linearity, limits of detection and quantification, accuracy and precision, method robustness and solution stability. The linearity of the calibration curves for mono- and di-TPGS 1000 from both sources was found to be good (r(2)>0.9996). The recovery values were from 94.6% to 103.3% for mono-TPGS, and 93.5% to 103.3% for di-TPGS. This method could be successfully used in the direct quantification of mono- and di-TPGS in TPGS 1000 mixture using TPGS standards with similar molecular mass distributions although derived from different sources.
