Significant Shifts in Microbial Communities Associated with Scleractinian Corals in Response to Algae Overgrowth.

Microbes play a key role in reef dynamics, mediating the competition between scleractinian corals and benthic algae; however, major shifts in bacterial communities among coral species in response to increases in the abundance of algae are not well understood. We investigated the taxonomic composition of coral-associated microbial communities under algae-overgrowth conditions using 16S rRNA gene sequencing. The results showed that non-algal (i.e., healthy) tissue (HH) had lower bacterial abundance and diversity than tissue collected from the coral-algae interface boundary (HA) and areas of algae growth (AA). Specifically, the HA and AA samples had higher relative abundances of Saprospiraceae, Rhodobacteraceae, and Alteromonadaceae. Compared with Platygyra sp. and Montipora sp., the physiological response of Pocillopora sp. was more intense under algae-induced stress based on microbial gene function prediction. Our results indicate that algal pressure can significantly alter the microbial community structure and function of coral ecosystems. Our data thus provide new insight into the relationship between corals and their microbiome under environmental stress.

Selective Modification of Halloysite Nanotubes with 1-Pyrenylboronic Acid: A Novel Fluorescence Probe with Highly Selective and Sensitive Response to Hyperoxide.

A novel fluorescence probe based on modified halloysite nanotubes (HNTs) by using 1-pyrenylboronic acid selectively grafted onto the inner surface of lumen was successfully achieved. The solid-state nuclear magnetic resonance ((13)C and (11)B), X-ray photoelectron spectroscopy (XPS) and Fourier transform infrared (FTIR) confirmed that the boronic acid group only binds to alumina at the tube lumen and does not bind the tube's outer siloxane surface. The modified HNTs (HNTs-PY) inherit the spectroscopic properties relating to the pyrene units. Interestingly, the established Al-O-B linkage gives the H2O2-sensitivity to pyrene grafted tubes. HNTs-PY exhibits a highly specific "turn-off" response for hyperoxide over other reactive oxygen species (ROS) and oxidative ions owing to their chemoselective boronate-to-phenol switch. The "turn-off" response can even be tracked when the additional amount of H2O2 was limited to 1 × 10(-6) mol. Thus, the selective modification method under mild conditions for the design of novel organic-inorganic hybrid fluorescence probe may open up a broader application as well as for identification and diagnosis.