ABSTRACT
Genomic DNA was extracted from 1 038 peripheral blood samples from HIV-infected individuals in Henan Province. One-step single-tube nested PCR was performed to amplify the 529 bp repeating sequences of Toxoplasma gondii. Of the 1 038 samples ï¼762 from males and 276 from femalesï¼, 66 showed positive PCR results, with a positive rate of 6.4%. The PCR positive rate in males and females was 6.3% ï¼48/762ï¼ and 6.5% ï¼18/276ï¼ respectively. The PCR positive rate in the married HIV individuals was 4.9%ï¼25/508ï¼, and that in unmarried, divorced and widowed HIV individuals was 7.7% ï¼41/530ï¼ï¼χ2 = 3.451, P> 0.05ï¼. The PCR positive rate in HIV individuals with a high-school educational level or above was 6.9%ï¼34/489ï¼, and that in those below the high-school level was 5.8% ï¼32/549ï¼ï¼χ2 = 0.545, P> 0.05ï¼. The highest infection rate was in the age group of 20-40 yearsï¼7.6%, 31/410ï¼. In addition, the Toxoplasma infection rate in those with and without a history of venereal diseases, and those with an unknown history was 8.0%ï¼9/113ï¼, 6.5%ï¼50/773ï¼ and 4.6%ï¼7/152ï¼ respectively ï¼χ2 = 0.355, P> 0.05ï¼.
