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Bing Du Xue Bao ; 32(6): 752-7, 2016 11.
Article in Chinese | MEDLINE | ID: mdl-30004648

ABSTRACT

We investigated the infection and molecular-epidemiologic characteristics of human astrovirus (HAstV) of hospitalized infants in Kunming City from the year 2013 to 2014.Infection and genotype of HAstV of 63 samples of diarrheal feces and 42 controls were analyzed by reverse transcription-polymerase chain reaction(RT-PCR).The complete genome sequence of a HAstV strain was amplified and sequenced. The positive rate of HAstV in 63 feces samples was 41.27%(26/63).The main circulating genotype of HAstV was HAstV1.Only 1sample was positive for HAstV in 42controls(2.38%).A complete genome sequence of the HAstV strain was identified as HAstV1 by phylogenetic analyses. These data provide an important theoretical basis for the control of viral diarrhea in infants in Kunming City.


Subject(s)
Astroviridae Infections/virology , Mamastrovirus/genetics , Mamastrovirus/isolation & purification , Astroviridae Infections/epidemiology , Astroviridae Infections/therapy , China/epidemiology , Diarrhea/epidemiology , Diarrhea/therapy , Diarrhea/virology , Feces/virology , Female , Genotype , Hospitalization/statistics & numerical data , Humans , Infant , Male , Mamastrovirus/classification , Molecular Epidemiology , Phylogeny
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