1.
Anal Biochem
; 233(1): 31-5, 1996 Jan 01.
Article
in English
| MEDLINE
| ID: mdl-8789143
ABSTRACT
We report a sodium dodecyl sulfate-polyacrylamide gel electrophoresis protocol for the reliable separation, with high resolution, of myosin heavy chain isoforms in adult avian (chicken) and mammalian (mouse) skeletal muscles. The sample preparation time can be relatively short, thereby minimizing endogenous proteolytic activity which may otherwise result in dispersed and spurious bands. Inclusion of 2-mercaptoethanol in the upper electrode buffer greatly improves band resolution. Glycerol is commonly included in the reported protocols for myosin heavy chain separation and our results demonstrate that the concentration of glycerol employed can have a marked effect on the relative order of migration among myosin heavy chain isoforms.