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1.
Placenta ; 36(1): 18-26, 2015 Jan.
Article in English | MEDLINE | ID: mdl-25468543

ABSTRACT

INTRODUCTION: Human term placenta has attracted increasing attention as an alternative source of stem cells for regenerative medicine since it is accessible without ethical objections. The amniotic membrane (AM) contains at least two stem cell types from different embryological origins: ectodermal amniotic epithelial stem cells, and mesodermal mesenchymal stromal cells. Among the second group we studied the characteristics of amniotic mesenchymal cells (AMC) versus the ones enriched for the commonly used surface marker c-Kit (amniotic progenitor/stem cells-ASC), a stem cell factor receptor with crucial functions in a variety of biological systems and presents in early progenitors of different origin, as been already demonstrated in the enriched chorionic stem cells. METHODS: After isolation, cells from the amniotic membranes (amniotic cells-AC) were selected for c-Kit (ASC) and compared these cells with c-Kit unselected (AMC), evaluating the expression of other stem cell markers (Oct-4, Tra-1-81, SSEA-4), CD271 and Slug. RESULTS: Immunofluorescence analysis showed that ASC cells exhibited greater stem cell marker expression and included more CD271 and Slug positive cells. This was consistent with the interpretation that c-Kit enriched AC show greater stemness capacity compared to c-Kit unselected AMC. DISCUSSION: AMC and ASC can both differentiate into various cell types including adipogenic, osteogenic, chondrogenic, neurogenic and hepatic lineages, but the enrichment in c-Kit improved stemness and differentiation potential of ASC.


Subject(s)
Amnion/cytology , Cell Differentiation/physiology , Mesenchymal Stem Cells/cytology , Proto-Oncogene Proteins c-kit/metabolism , Stem Cells/cytology , Amnion/metabolism , Epithelial Cells/cytology , Epithelial Cells/metabolism , Female , Humans , Mesenchymal Stem Cells/metabolism , Octamer Transcription Factor-3/metabolism , Placenta/cytology , Placenta/metabolism , Pregnancy , Stem Cells/metabolism
2.
Placenta ; 34(7): 526-35, 2013 Jul.
Article in English | MEDLINE | ID: mdl-23643069

ABSTRACT

OBJECTIVE: Human term placenta (HTP) has attracted increasing attention as an alternative source of stem cells for regenerative medicine since the amniochorionic membrane harbors stem cells populations that are easily accessible, abundantly available without ethical objections. In the chorionic side of HTP we found a progenitor perivascular "niche" in which rare cells co-express Oct-4 and c-Kit. We investigated the stem cell characteristics and differentiation potential of a chorionic derived population enriched in c-Kit(+) cells and compared this to the unenriched population. STUDY DESIGN: Cells, isolated from the chorion of HTP, were expanded and enriched in c-Kit(+) cells (Chorionic Stem Cells-CSC). Histological staining, immunofluorescence, Western blot and flow cytometry were used to verify the stem cells characteristics of the populations and to compare the differentiation capability towards mesodermal and neural lineages in vitro. RESULTS: The expression of the pluripotent marker Oct-4 was greater in the CSCs compared to the unselected cells (Chorionic Cell-CC) but both Oct-4 and c-Kit expression decreased during passages. After differentiation, CSC displayed stronger chondrogenic and osteogenic potential and a greater adipogenic forming capacity compared to unselected ones. CSC differentiated better into immature oligodendrocytes while CC showed a neuronal progenitor differentiation potential. Moreover, both populations were able to differentiate in hepatogenic lineage. CONCLUSION: CSC display improved Oct-4 expression and a high differentiation potential into mesodermal lineages and oligodendrocytes.


Subject(s)
Cell Differentiation , Chorion/cytology , Embryonic Stem Cells/metabolism , Octamer Transcription Factor-3/biosynthesis , Proto-Oncogene Proteins c-kit/biosynthesis , Adult , Cell Lineage , Chorion/metabolism , Embryonic Stem Cells/cytology , Female , Humans , Mesoderm/cytology , Nervous System/embryology , Placenta/cytology , Pregnancy
3.
Life Sci ; 92(20-21): 993-1003, 2013 May 30.
Article in English | MEDLINE | ID: mdl-23583571

ABSTRACT

AIMS: The phytoestrogen Ferutinin plays an important role in prevention of osteoporosis caused by ovariectomy-induced estrogen deficiency in rats, but there is no evidence of its effect on osteoblastic differentiation in vitro. In this study we investigated the effect of Ferutinin on proliferation and osteoblastic differentiation of two different human stem cells populations, one derived from the amniotic fluid (AFSCs) and the other from the dental pulp (DPSCs). MAIN METHODS: AFSCs and DPSCs were cultured in a differentiation medium for 14 or 21days with or without the addition of Ferutinin at a concentration ranging from 10(-11) to 10(-4)M. 17ß-Estradiol was used as a positive drug at 10(-8)M. Cell proliferation and expression of specific osteoblast phenotype markers were analyzed. KEY FINDINGS: MTT assay revealed that Ferutinin, at concentrations of 10(-8) and 10(-9)M, enhanced proliferation of both AFSCs and DPSCs after 72h of exposure. Moreover, in both stem cell populations, Ferutinin treatment induced greater expression of the osteoblast phenotype markers osteocalcin (OCN), osteopontin (OPN), collagen I, RUNX-2 and osterix (OSX), increased calcium deposition and osteocalcin secretion in the culture medium compared to controls. These effects were more pronounced after 14days of culture in both populations. SIGNIFICANCE: The enhancing capabilities on proliferation and osteoblastic differentiation displayed by the phytoestrogen Ferutinin make this compound an interesting candidate to promote bone formation in vivo.


Subject(s)
Amniotic Fluid , Benzoates/pharmacology , Cell Differentiation/drug effects , Cell Proliferation/drug effects , Cycloheptanes/pharmacology , Dental Pulp/drug effects , Osteoblasts/drug effects , Sesquiterpenes/pharmacology , Stem Cells/drug effects , Biomarkers/metabolism , Blotting, Western , Bridged Bicyclo Compounds/pharmacology , Cells, Cultured , Culture Media , Dental Pulp/cytology , Fluorescent Antibody Technique , Humans , Microscopy, Confocal , Osteoblasts/cytology , Stem Cells/cytology
4.
Eur J Histochem ; 54(4): e46, 2010 Nov 10.
Article in English | MEDLINE | ID: mdl-21263745

ABSTRACT

The aim of this study was to characterize the in vitro osteogenic differentiation of dental pulp stem cells (DPSCs) in 2D cultures and 3D biomaterials. DPSCs, separated from dental pulp by enzymatic digestion, and isolated by magnetic cell sorting were differentiated toward osteogenic lineage on 2D surface by using an osteogenic medium. During the differentiation process, DPSCs express specific bone proteins like Runx-2, Osx, OPN and OCN with a sequential expression, analogous to those occurring during osteoblast differentiation, and produce extracellular calcium deposits. In order to differentiate cells in a 3D space that mimes the physiological environment, DPSCs were cultured in two distinct bioscaffolds, Matrigel™ and Collagen sponge. With the addition of a third dimension, osteogenic differentiation and mineralized extracellular matrix production significantly improved. In particular, in Matrigel™ DPSCs differentiated with osteoblast/osteocyte characteristics and connected by gap junction, and therefore formed calcified nodules with a 3D intercellular network. Furthermore, DPSCs differentiated in collagen sponge actively secrete human type I collagen micro-fibrils and form calcified matrix containing trabecular-like structures. These neo-formed DPSCs-scaffold devices may be used in regenerative surgical applications in order to resolve pathologies and traumas characterized by critical size bone defects.


Subject(s)
Calcification, Physiologic/physiology , Dental Pulp/cytology , Extracellular Matrix/metabolism , Osteogenesis/physiology , Stem Cells/physiology , Adolescent , Adult , Blotting, Western , Cell Culture Techniques , Cell Differentiation , Cells, Cultured , Fluorescent Antibody Technique , Humans , Immunoenzyme Techniques , Young Adult
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