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1.
Int. j. lepr. other mycobact. dis ; 69(1): 21-25, Mar., 2001. ilus
Article in English | Sec. Est. Saúde SP, HANSEN, Hanseníase Leprosy, SESSP-ILSLACERVO, Sec. Est. Saúde SP | ID: biblio-1226994

ABSTRACT

Polymerase chain reaction (PCR)-restriction fragment length polymorphism analysis (PRA) which relies on the amplification of a 439-bp portion of the hsp65 gene present in all mycobacteria, followed by two distinct digestions (with BstEII and HaeIII) of the PCR product, offers a rapid and easy alternative that allows identification of the species without the need for specialized equipment. Wild leprosy in the nine-banded armadillo (Dasypus novemcinctus) is characterized by the presence of multiple bacilli in internal organs such as lymph nodes, spleen and liver, as well as in nerves and skin. We could observe this in 9 out of 132 animals captured in Corrientes, Argentina, an area endemic for leprosy in humans. Mycobacterium leprae were recognized in those naturally infected animals through different techniques. Three samples of extracted DNA of the mycobacteria present in the spleen, liver and popliteal lymph node of a naturally infected animal during the Experimental Program in Armadillo (PEA) and three samples of human lepromas were processed by PRA. The patterns of the six samples analyzed were identical and were characteristic of M. leprae. These studies, made for the first time in Argentina, corroborate the initial discoveries in South America made by our investigative group on the detection of armadillos naturally infected with the Hansen bacillus.


Subject(s)
Animals , Mycobacterium leprae/physiology , Mycobacterium leprae/genetics , Mycobacterium leprae/immunology , Reverse Transcriptase Polymerase Chain Reaction/methods
2.
Rev. chil. anat ; 17(1): 27-31, 1999. ilus
Article in Spanish | LILACS | ID: lil-255241

ABSTRACT

Los orígenes de la arteria intercostal superior apartir de la arteria axilar o bien de la arteria cervucal profunda (ACP) descriras por CHAVEAU & ARLONG (1905 por ciento) como anomalías, no fueron observados en nuestra experiencia. El desplazamiento de la arteria escapular dorsal hacia el 3º espacio intercostal y de la arteria cervical profunda hacia el 2º espacio intercostal descritos por este autor, fueron observados en los casos examinados. A su vez, el origen en común de la arteria cervical superficial y arteria torácica externa no fue observado. Si bien la ATE puede originarse de la arteria torácica interna como describe CHAVEAU & ARLOING, en nuestras diseccionesfue observado como colateral de la arteria torácica interna. El comportamiento de la arteria costocervical y arteria cervical profunda cuanto a su origen, a partir de un tronco común, Coincide con las variaciones descritas por GETTY, (1982). Las aseveraciones efectuadas por BOSSI et al., (1903) sobre el mayor porcentaje de variaciones en la arteria costocervical y arteria torácica externa, no coinciden con nuestras descripciones. Existe coincidencia con BOSSI et al. En cuanto al uso del término <> y no el de arteria subclavia en perisodáctilos domésticos, ya que no existe una clavícula perfectamente desarrollada que justifique tal denominación. Los datos obtenidos del matrial procesado, indican variaciones a nivel de las colaterales intatóracicas, entre las que merecen especial mención las arterias costo cervical y cervical profunda, en las cuales fue observado el mayor número de variaciones, en su origen, trayecto y terminación. El conocimiento de sus variaciones es importante tanto en el campo de la Anatomía como el de la Cirugía , ya que permite evitar errores, a veces insalvables, durante el acto quirúrgico de patologías de la región torácica


Subject(s)
Animals , Equidae/anatomy & histology , Brachiocephalic Trunk/anatomy & histology , Axillary Artery/anatomy & histology , Mammary Arteries/anatomy & histology , Brachiocephalic Trunk/abnormalities , Vertebral Artery/anatomy & histology
3.
Bol. chil. parasitol ; 53(3/4): 70-2, jul.-dic. 1998. ilus, tab
Article in Spanish | LILACS | ID: lil-245374

ABSTRACT

A case of caecum nematodiasis is described in a guinea fowl (Numida melagris) from the Municipal Zoo, Presidencia Roque Saenz Peña (Chaco) Argentina. Nematodes obtained from the caecum were observed in optic microscopy. According to their morphometric characteristics and location in the definitive host, were identified as belonging to the family heterakidae, species Heterakis gallinarum, (Schrank, 1788) Maden, 1949


Subject(s)
Animals , Nematoda/isolation & purification , Poultry/parasitology , Argentina , Disease Vectors , Intestines/parasitology , Nematoda/anatomy & histology , Nematoda/pathogenicity , Nematode Infections
4.
Bol. chil. parasitol ; 52(3/4): 70-2, dic. 1997. ilus
Article in Spanish | LILACS | ID: lil-210469

ABSTRACT

A case of gastric nematodiasis is describes in a gineafowl (Numida meleagris) from the Municipal Zoo, Presidencia Roque Saenz Peña (Chaco) Argentina, nematodes obtained from the glandular stomach were observed in optic microscopy. According to their morphometric characteristies and location in the definitivo host, were identified as belonging to the family Acuariidae, subfamily Acuariinae, species Dispharynx nasuta Rudolphi, 1819


Subject(s)
Animals , Stomach, Avian/physiopathology , Nematoda/isolation & purification , Argentina , Crustacea/parasitology , Nematoda/anatomy & histology , Host-Parasite Interactions
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