ABSTRACT
INTRODUCTION: Sterilisation using peracetic acid (PAA) has been advocated for orthodontic elastic bands. However, cane-loaded elastomeric ligatures can also become contaminated during processing, packaging, and manipulation before placement in the oral cavity and are therefore susceptible, and possible causes, of cross-contamination. AIM: To test the hypothesis that 0.25% peracetic acid (PAA), following the sterilisation of elastomers, influences the cytotoxicity of elastomeric ligatures on L929 cell lines. MATERIALS AND METHODS: Four hundred and eighty silver elastomeric ligatures were divided into 4 groups of 120 ligatures to produce, Group TP (latex natural, bulk pack, TP Orthodontics), Group M1 (Polyurethane, bulk pack, Morelli), Group M2 (Polyurethane, cane-loaded, Morelli) and Group U (Polyurethane, cane-loaded, Uniden). Of the 120 ligatures in each group, 100 were sterilised in 0.25% PAA at time intervals (N = 20) of 1 hour, 2 hours, 3 hours, 4 hours and 5 hours. The 20 remaining elastomeric ligatures in each group were not sterilised and served as controls. Cytotoxicity was assessed using L929 cell lines and a dye-uptake method. Analysis of variance (ANOVA), followed by the Tukey post hoc test (p < 0.05) determined statistical relevance. RESULTS: There was a significant difference between TP, Morelli and Uniden elastomerics (p < 0.05), but no difference between the two types of Morelli elastomerics at the 1 hour time interval. In addition, there was a significant difference between Group CC and the other groups assessed, except between Groups CC and TP at the 1 hour time interval. The non-sterilised elastomeric ligatures showed similar cell viability to that observed after 1 hour of standard sterilisation. CONCLUSION: PAA did not significantly influence the cytotoxicity of elastomeric ligatures after a sterilisation time of 1 hour and is therefore recommended for clinical use.
Subject(s)
Dental Disinfectants/therapeutic use , Elastomers/toxicity , Orthodontic Appliances , Peracetic Acid/therapeutic use , Sterilization/methods , Animals , Cell Culture Techniques , Cell Line , Cell Survival/drug effects , Coloring Agents , Dental Disinfectants/chemistry , Elastomers/chemistry , Fibroblasts/drug effects , Latex/chemistry , Latex/toxicity , Materials Testing , Neutral Red , Peracetic Acid/chemistry , Polyurethanes/chemistry , Polyurethanes/toxicity , Rats , Time FactorsABSTRACT
INTRODUCTION: The purposes of this study were to determine the ideal sites for placement of orthodontic mini-implants in mandibular interradicular spaces by using computed tomography (CT) and to suggest length, diameter, and angulation of the mini-implants. METHODS: CT scans were performed on 15 dry human mandibles with 1-mm tomography slices. Measurements were made at 3, 5, 7, 9, and 11 mm heights from the bone crest. Bone thickness was obtained for the buccolingual, lingual cortex, and buccal cortex areas. The mesiodistal interradicular distance and the distance from the bone crest to the mental foramen were also measured. Simulated placement of 1.5 x 9 mm mini-implants was performed in the tomographic images at angulations 10 degrees , 20 degrees , and 30 degrees . Twenty-four 1.5 x 9 mm mini-implants were then placed in the mandibles, and a new set of CT scans was obtained. Mandibles with implants were sectioned, enabling direct observation. RESULTS: Based on 3000 measurements, means and standard deviations were obtained. The thickness of the mandibular alveolar bone in the cortical buccal and lingual areas, and the interradicular distances increased from the cervical toward the apical aspects. In descending order, the widest spaces were found between the first and second molars, the second premolars and the first molars, and the first and second premolars. Between the premolars, caution should be exercised starting at 9 mm from the bone crest because of the mental foramen. Between the incisors, the placement of interradicular mini-implants is not feasible. Between the first premolars and the canines, no appropriate region was found. Between the lateral incisor and the canine, at a height of 11 mm, a device can be placed but only with utmost care. CONCLUSIONS: The most convenient site for implant placement in a mandible was between the first and second molars, with a 10 degrees to 20 degrees inclination, but orthodontic mini-implants should not exceed 1.5 mm in diameter and 6 mm in length.
