ABSTRACT
The aim of the study was to determine the prevalence of Treponema denticola in saliva of periodontally diseased and healthy patients and its relationship with the periodontal status. A 16S rRNA-based polymerase chain reaction detection method was used to determine the prevalence of T. denticola in whole saliva samples from patients with chronic periodontitis (CP, n = 37), aggressive periodontitis (AgP, n = 24), and healthy subjects (n = 28). The periodontal status of each subject was assessed by criteria based on probing depth, clinical attachment loss, and extent of periodontal breakdown. Risk factors were assessed individually and adjusted for confounding using a binary logistic regression model. The results showed that the prevalence of T. denticola in CP patients was significantly higher than those in healthy and AgP subjects (P < 0.05). Odds ratio analysis revealed a positive association for CP group/T. denticola-positive and smoking/T. denticola-positive subjects. Furthermore, all clinical measurements were significantly greater (P < 0.05) for T. denticola-positive subjects compared to T. denticola-negative subjects. After binary logistic regression analysis, both T. denticola and smoking were independently and strongly associated with development of CP. It was concluded that when used in conjunction with an optimized clinical examination protocol, this assay may offer a rapid, useful, and cost-effective tool for monitoring the presence of T. denticola in noninvasive clinical samples from both healthy and diseased patients and correlating it with the amount and extent of periodontal breakdown.
Subject(s)
Periodontitis/microbiology , Saliva/microbiology , Treponema denticola/isolation & purification , Adolescent , Adult , Aged , Chronic Disease , Colony Count, Microbial , Confounding Factors, Epidemiologic , Female , Humans , Male , Middle Aged , Periodontal Attachment Loss/microbiology , Periodontal Index , Periodontal Pocket/microbiology , Periodontium/microbiology , Polymerase Chain Reaction , RNA, Ribosomal, 16S/analysis , Risk Factors , Smoking , Treponema denticola/geneticsABSTRACT
Son diversos los factores de riesgo asociados con la aparición de cáncer bucal, entre ellos algunos hábitos como el consumo de cigarrillo y alcohol. Este estudio descriptivo retrospectivo evaluó diferentes variables como edad, sexo, consumo de bebidas alcohólicas y cigarrillos entre los pacientes con diangóstico de cáncer bucal en la Unidad de Cirugía Maxilofacial y Estomatología del HUSVP en Medellín, Colombia. Se encontró una mayor prevalencia de cáncer bucal en el sexo masculino, principalmente en mayores de 45 años con un aumento significativo después de los 60 años, siendo el principal tipo de cáncer el carcinoma escamocelular (88 por ciento) y la ubicación más frecuente en el borde lateral de lengua. El 36 por ciento de los pacientes consumía simultáneamente alcohol y cigarrillo y sólo el 23 por ciento eran alcohólicos puros. No es posible establecer relación causa-efecto entre consumo de licor y cáncer bucal, aunque se observa que es mayor el factor de riesgo cuando se consume simultáneamente alcohol y cigarrillo. Debe investigarse otro tipo de hábitos que puedan asociarse a la aparición de cáncer bucal debido al número alto de personas con la enfermedad y sin hábitos conocidos en nuestra población
