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Allergy ; 39(2): 125-33, 1984 Feb.
Article in English | MEDLINE | ID: mdl-6230948

ABSTRACT

Poodle and Alsatian dog dandruff extracts were characterized by crossed immunoelectrophoresis (CIE) and crossed radioimmunoelectrophoresis (CRIE) using sera from 24 individuals clinically sensitive to dogs. By using a system with intermediate gel in immunoelectrophoresis, the content of dander-specific and serum-specific allergens was established. 29 antigens (18 dander-specific and 11 serum-specific) were identified in the mixed breed Poodle/Alsation dandruff extract. Of these, 24 antigens were radiostained in CRIE. 16 allergens were dander specific and the remaining eight were serum specific. Positive dog dander RAST (e5 and Poodle/Alsatian dandruff extract) results were observed in the tested dog hypersensitive subjects. Our results suggest that the mixture of Poodle/Alsatian dandruff extract may be a suitable preparation for the diagnosis and treatment of dog allergy.


Subject(s)
Allergens/analysis , Dermatitis, Seborrheic/veterinary , Dog Diseases/immunology , Animals , Dermatitis, Seborrheic/immunology , Dogs , Immunoelectrophoresis/methods , Radioallergosorbent Test
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