ABSTRACT
Nocardia is an opportunistic pathogen that causes respiratory infections in immunocompromised patients. The aim of this study was to analyze the epidemiology, clinical significance and antimicrobial susceptibility of Nocardia species isolated from eight children with cystic fibrosis. The isolated species were identified as Nocardia farcinica, Nocardia transvalensis, Nocardia pneumoniae, Nocardia veterana and Nocardia wallacei. N. farcinica was isolated in three patients and all of them presented lung affectation with a chronic colonization and pneumonia. N. farcinica showed resistance against gentamicin, tobramycin, cefotaxime, but was susceptible to trimethoprim-sulfamethoxazole and amikacin. N. transvalensis, which was isolated from two patients, showed an association with chronic colonization. N. transvalensis was resistant to tobramycin and amikacin, but susceptible to ciprofloxacin, trimethoprim-sulfamethoxazole and cefotaxime. N. veterana, N. pneumoniae and N. wallacei were isolated from three different patients and appeared in transitory lung colonization. N. veterana and N. pneumoniae were susceptible to imipenem, trimethoprim-sulfamethoxazole, amikacin, tobramycin, and cefotaxime. N. wallacei was resistant to amikacin, tobramycin, imipenem, and trimethoprim-sulfamethoxazole and susceptible to ciprofloxacin and cefotaxime. All the isolates were identified up to species level by 16S rRNA gene sequencing. The presence of Nocardia in the sputum of patients with cystic fibrosis is not always an indication of an active infection; therefore, the need for a treatment should be evaluated on an individual basis. The detection of multidrug-resistant species needs molecular identification and susceptibility testing, and should be performed for all Nocardia infections.
Subject(s)
Cystic Fibrosis/complications , Nocardia Infections/microbiology , Nocardia/classification , Nocardia/drug effects , Opportunistic Infections , Adolescent , Anti-Bacterial Agents/pharmacology , Anti-Bacterial Agents/therapeutic use , Child , Cystic Fibrosis/drug therapy , Cystic Fibrosis/mortality , Female , Humans , Male , Microbial Sensitivity Tests , Nocardia/isolation & purification , Nocardia Infections/drug therapy , Nocardia Infections/mortality , Treatment OutcomeABSTRACT
Early and accurate diagnosis of invasive aspergillosis (IA) is one of the most critical steps needed to efficiently treat the infection and reduce the high mortality rates that can occur. We have previously found that the Aspergillus spp. secondary metabolite, bis(methylthio)gliotoxin (bmGT), can be detected in the serum from patients with possible/probable IA. Thus, it could be used as a diagnosis marker of the infection. However, there is no data available concerning the sensitivity, specificity and performance of bmGT to detect the infection. Here, we have performed a prospective study comparing bmGT detection with galactomannan (GM), the most frequently used and adopted approach for IA diagnosis, in 357 sera from 90 episodes of patients at risk of IA. Our results, involving 79 patients that finally met inclusion criteria, suggest that bmGT presents higher sensitivity and positive predictive value (PPV) than GM and similar specificity and negative predictive value (NPV). Importantly, the combination of GM and bmGT increased the PPV (100 %) and NPV (97.5 %) of the individual biomarkers, demonstrating its potential utility in empirical antifungal treatment guidance and withdrawal. These results indicate that bmGT could be a good biomarker candidate for IA diagnosis and, in combination with GM, could result in highly specific diagnosis of IA and management of patients at risk of infection.
Subject(s)
Biomarkers/blood , Gliotoxin/analogs & derivatives , Invasive Pulmonary Aspergillosis/diagnosis , Aged , Aged, 80 and over , Female , Galactose/analogs & derivatives , Gliotoxin/blood , Humans , Male , Mannans/blood , Middle Aged , Predictive Value of Tests , Prospective Studies , Sensitivity and SpecificityABSTRACT
Hypericin is a natural photosensitizer used in photodynamic therapy (PDT), which has shown in vitro antifungal effect against Candida spp. The aim of this study was to evaluate the in vitro fungicidal effect of hypericin-PDT on dermatophytes. Trichophyton rubrum and Trichophyton mentagrophytes strains were incubated with different concentrations of hypericin for different times and exposed to light-emitting diode lamp (602 ± 10 nm, 10.3 mW cm(-2), and fluence 37 J cm(-2)). Using the optimal incubation time, 60 min, a 3-log fungicidal effect was achieved with hypericin concentration ranges of 10-20 µM for T. rubrum and 20-50 µM for T. mentagrophytes (p = 0.95). Confocal fluorescence microscopy showed the localization of hypericin inside the dermatophytes diffusely distributed in the cytoplasm of conidia and hyphae and outside the nucleus. In conclusion, hypericin-PDT has a fungicidal effect in vitro on dermatophytes. Hypericin seems to be a promising photosensitizer to treat localized dermatophytic infections such as tinea pedis and onychomycosis.
Subject(s)
Antifungal Agents/pharmacology , Light , Perylene/analogs & derivatives , Photosensitizing Agents/pharmacology , Trichophyton/drug effects , Trichophyton/radiation effects , Anthracenes , Colony Count, Microbial , Humans , Microbial Viability/drug effects , Microbial Viability/radiation effects , Perylene/pharmacology , Time FactorsSubject(s)
Drug Resistance, Multiple, Bacterial/genetics , Mycobacterium tuberculosis/drug effects , Tuberculosis, Multidrug-Resistant/epidemiology , Tuberculosis, Multidrug-Resistant/microbiology , Genotype , Mycobacterium Infections/epidemiology , Mycobacterium tuberculosis/classification , Mycobacterium tuberculosis/genetics , Tuberculosis, Multidrug-Resistant/drug therapy , Venezuela/epidemiologySubject(s)
Abdominal Pain/blood , Fever/blood , Abdominal Pain/diagnosis , Adolescent , Animals , Antimalarials/therapeutic use , Azure Stains , Borrelia Infections/diagnosis , Borrelia Infections/drug therapy , Doxycycline/therapeutic use , Enzyme-Linked Immunosorbent Assay , Fever/diagnosis , Hematologic Tests , Humans , Male , Ornithodoros/microbiology , Senegal , SpainABSTRACT
No disponible
Subject(s)
Animals , Adolescent , Male , Humans , Spain , Senegal , Abdominal Pain , Ornithodoros , Borrelia Infections , Antimalarials , Azure Stains , Doxycycline , Fever , Enzyme-Linked Immunosorbent Assay , Hematologic TestsABSTRACT
No disponible
Subject(s)
Adult , Female , Humans , Urine , Urinary Tract Infections , Bacterial Typing Techniques , Down Syndrome , Corynebacterium , Corynebacterium InfectionsABSTRACT
No disponible