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1.
Cancer Res ; 71(6): 2140-51, 2011 Mar 15.
Article in English | MEDLINE | ID: mdl-21257709

ABSTRACT

Alterations in the ErbB family of growth factor receptors, their signaling components, and mutational activation of Ras proteins are major contributors to malignant transformation. Recently, mutant Ras was shown to be capable of activating ErbB receptors in a ligand-independent manner. Furthermore, it was observed that nucleolin, a transcriptional regulator and ribosome biogenesis factor, can bind both K-Ras and the cytoplasmic tail of ErbB receptors to enhance ErbB receptor activation. However, the functional significance of these interactions to cancer pathogenesis has not been probed. Here, we show that endogenous nucleolin interacts simultaneously in vivo with endogenous Ras and ErbB1 (EGFR) in cancer cells. The C-terminal 212 amino acids of nucleolin were determined to be sufficient to interact with ErbB1 and all Ras protein isoforms (H-, N-, and K-Ras). Nucleolin partially colocalizes with Ras at the plasma membrane. Moreover, activated but not wild-type Ras facilitates nucleolin interaction with ErbB1 and stabilizes ErbB1 receptor levels. Most importantly, these three oncogenes synergistically facilitate anchorage-independent cell growth in vitro and tumor growth in vivo. Our findings suggest strategies to target nucleolin as a general approach to inhibiting ErbB- and Ras-driven cancers.


Subject(s)
ErbB Receptors/metabolism , Mutant Proteins/metabolism , Phosphoproteins/metabolism , RNA-Binding Proteins/metabolism , ras Proteins/metabolism , Animals , Cell Line , Cell Line, Tumor , Cell Membrane/metabolism , Cell Transformation, Neoplastic/genetics , ErbB Receptors/genetics , Green Fluorescent Proteins/genetics , Green Fluorescent Proteins/metabolism , HEK293 Cells , Humans , Immunoblotting , Immunoprecipitation , Mice , Mice, Nude , Microscopy, Confocal , Mutant Proteins/genetics , Mutation , Neoplasms, Experimental/genetics , Neoplasms, Experimental/metabolism , Neoplasms, Experimental/pathology , Phosphoproteins/genetics , Protein Binding , RNA-Binding Proteins/genetics , Transplantation, Heterologous , ras Proteins/genetics , Nucleolin
2.
Exp Cell Res ; 312(20): 4056-69, 2006 Dec 10.
Article in English | MEDLINE | ID: mdl-17056038

ABSTRACT

During hematogenous cancer metastasis, tumor cells separate from a primary mass, enter the bloodstream, disperse throughout the body, migrate across vessel walls, and generate distant colonies. The later steps of metastasis superficially resemble leukocyte extravasation, a process initiated by selectin-mediated cell tethering to the blood vessel wall followed by integrin-mediated arrest and transendothelial migration. Some cancer cells express P-selectin ligands and attach to immobilized P-selectin, suggesting that these cells can arrest in blood vessels using sequential selectin- and integrin-mediated adhesion, as do leukocytes. We hypothesize that selectin binding may regulate subsequent integrin-mediated steps in metastasis. Using a model system of cultured Colo 320 human colon adenocarcinoma cells incubated with soluble P-selectin-IgG chimeric protein, we have found that P-selectin can stimulate activation of the alpha(5)beta(1) integrin resulting in a specific increase of adhesion and spreading of these cells on fibronectin substrates. P-selectin binding also induced activation of p38 mitogen-activated protein kinase (p38 MAPK) and phosphatidylinositol 3-kinase (PI3-K). PI3-K inhibitors blocked P-selectin-mediated integrin activation, cell attachment, and cell spreading. Inhibition of p38 MAPK activation blocked cell spreading, but not cell attachment. P-selectin binding also resulted in formation of a signaling complex containing PI3-K and p38 MAPK. These results suggest that P-selectin binding to tumor cells can activate alpha(5)beta(1) integrin via PI3-K and p38 MAPK signaling pathways leading to increased cell adhesion. We propose that P-selectin ligands are important tumor cell signaling molecules that modulate integrin-mediated cell adhesion in the metastatic process.


Subject(s)
Adenocarcinoma/metabolism , Colonic Neoplasms/metabolism , Fibronectins/metabolism , Integrin alpha5beta1/metabolism , P-Selectin/pharmacology , Cell Adhesion , Cell Line, Tumor , Enzyme Activation , Humans , Integrin alpha5beta1/physiology , MAP Kinase Signaling System , Phosphatidylinositol 3-Kinases/metabolism , Protein Binding , p38 Mitogen-Activated Protein Kinases/metabolism
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