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1.
Tumour Biol ; 22(1): 19-26, 2001.
Article in English | MEDLINE | ID: mdl-11054023

ABSTRACT

The CYFRA 21-1 assay detects circulating fragments of cytokeratin 19, which is a sensitive marker for the diagnosis of lung cancers, particularly squamous cell carcinomas and adenocarcinomas. Epidermis-type proteins, such as cytokeratins 1, 2, 10/11 and 14 or filaggrin, are also expressed in squamous cell carcinomas. These could also be pertinent tumor markers, ideally as sensitive as CYFRA 21-1 and more specific for squamous cell lung cancer. To verify this hypothesis, using monoclonal antibodies produced in our laboratory, we developed immunoassays specific for these proteins. After optimization, the immunoassays were evaluated in sera from 91 controls and 138 patients with squamous cell lung cancer and compared to conventional tumor markers (CEA, SCC Ag and CYFRA 21-1). Less than 14% of the sera were above the lower limit of detection of the cytokeratin- and filaggrin-specific immunoassays. Moreover, part of these positive sera were induced by the presence of interfering heterophilic antibodies in sera. Thus, in patients with squamous cell lung cancer, we confirmed the high diagnostic sensitivity of CYFRA 21-1 (55.6%) but were unable to detect significant levels of epidermis-type cytokeratins or filaggrin.


Subject(s)
Antigens, Neoplasm/blood , Biomarkers, Tumor/blood , Carcinoma, Squamous Cell/blood , Intermediate Filament Proteins/blood , Keratins/blood , Lung Neoplasms/blood , Neoplasm Proteins/blood , Peptide Fragments/blood , Serpins , Antibodies, Monoclonal/immunology , Antibody Specificity , Antigens, Neoplasm/analysis , Antigens, Neoplasm/immunology , Biomarkers, Tumor/immunology , Calibration , Carcinoembryonic Antigen/analysis , Carcinoma, Squamous Cell/pathology , Cell Differentiation , Enzyme-Linked Immunosorbent Assay , Epidermis/chemistry , Filaggrin Proteins , Humans , Intermediate Filament Proteins/immunology , Keratin-19 , Keratins/immunology , Lung Diseases/blood , Lung Neoplasms/pathology , Neoplasm Proteins/immunology , Sensitivity and Specificity
3.
Br J Cancer ; 81(6): 1059-65, 1999 Nov.
Article in English | MEDLINE | ID: mdl-10576665

ABSTRACT

Carcinoembryonic antigen (CEA), carbohydrate antigens 15-3, 19-9 and 72-4 (CA 15-3, CA 19-9 and CA 72-4), cytokeratin 19 fragments (CYFRA 21-1), neuron-specific enolase (NSE) and squamous cell carcinoma antigen (SCC) were evaluated in pleural fluid for the diagnosis of malignant effusions. With a specificity of 99%, determined in a series of 121 benign effusions, the best individual diagnostic sensitivities in the whole series of 215 malignant effusions or in the subgroup of adenocarcinomas were observed with CEA, CA 15-3 and CA 72-4. As expected, a high sensitivity was obtained with SCC in squamous cell carcinomas and with NSE in small-cell lung carcinomas. CYFRA and/or CA 15-3 were frequently increased in mesotheliomas. Discriminant analysis showed that the optimal combination for diagnosis of non-lymphomatous malignant effusions was CEA + CA 15-3 + CYFRA + NSE: sensitivity of 94.4% with an overall specificity of 95%. In malignant effusions with a negative cytology, 83.9% were diagnosed using this association. The association CYFRA + NSE + SCC was able to discriminate adenocarcinomas from small-cell lung cancers. Regarding their sensitivity and their complementarity, CEA, CA 15-3, CYFRA 21-1, NSE and SCC appear to be very useful to improve the diagnosis of malignant pleural effusions.


Subject(s)
Biomarkers, Tumor/analysis , Pleural Effusion, Malignant/diagnosis , Adenocarcinoma/diagnosis , Adenocarcinoma/pathology , Adolescent , Adult , Aged , Aged, 80 and over , Carcinoma/diagnosis , Carcinoma/pathology , Carcinoma, Small Cell/diagnosis , Carcinoma, Small Cell/pathology , Carcinoma, Squamous Cell/diagnosis , Carcinoma, Squamous Cell/pathology , Child , Female , Humans , Lung Neoplasms/diagnosis , Lung Neoplasms/pathology , Male , Middle Aged , Pleural Effusion, Malignant/pathology , Retrospective Studies , Sensitivity and Specificity
4.
Ann Otolaryngol Chir Cervicofac ; 116(2): 78-84, 1999 May.
Article in French | MEDLINE | ID: mdl-10378036

ABSTRACT

Allergic aspergillar sinusitis is a very controversial clinical feature. We present results of a prospective study aimed at evaluating the reality of allergic aspergillar sinusitis in a nosologic and clinical point of vue. During a 5 months period, 31 patients underwent surgery: 21 sino-nasal polyposis, 5 chronic sinusitis without polyposis, 5 chronic sinusitis with radiologic images evocative of mycosis. The study was carried out using clinical criteria (per-operative discovery of glue-like, thickened and viscous aspect of the secretions), pathologic criteria (the presence of elements consitutive of allergic mucin), mycological criteria (direct examination and culture), and immunoallergic criteria (specific IgE for Aspergillus fumigatus, serology for Aspergillus fumigatus and Aspergillus flavus (IgM, IgG), skin tests for Aspergillus fumigatus). In three cases we suspect an allergic aspergillar sinusitis (one patient presenting a bilateral chronic sinusitis and two patients presenting a sinonasal polyposis). In two patients presenting a sinonasal polyposis, a allergic fungal sinusitis was suspected, fungal identification was not possible.


Subject(s)
Aspergillosis/complications , Aspergillus fumigatus/isolation & purification , Hypersensitivity/complications , Sinusitis/microbiology , Adult , Aged , Aged, 80 and over , Aspergillosis/diagnosis , Chronic Disease , Female , Humans , Hypersensitivity/diagnosis , Immunoglobulin G/immunology , Immunoglobulin M/immunology , Male , Middle Aged , Nasal Polyps/diagnosis , Nasal Polyps/microbiology , Prospective Studies , Severity of Illness Index , Sinusitis/diagnosis
5.
J Radiol ; 60(6-7): 443-5, 1979.
Article in French | MEDLINE | ID: mdl-501705

ABSTRACT

From one obstructive jaundice case examined, the Authors describe, radiologically, the result of a chronicle pancreatitis: pseudocyst with internal cysto-duodenal fistula and visualisation by radiographic examination; stenosis of second duodenum; aerobilia.


Subject(s)
Pancreatic Cyst/diagnostic imaging , Duodenal Diseases/diagnostic imaging , Humans , Intestinal Fistula/diagnostic imaging , Male , Middle Aged , Pancreatic Fistula/diagnostic imaging , Pancreatitis/diagnostic imaging , Radiography
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