ABSTRACT
Culture supernatants of splenocytes from C57BL/6 mice were exposed to 0.3, 1.0, and 3.0 microg/ml cordycepin plus 3.0 microg/ml lipopolysaccharide (LPS) to investigate the effects of cordycepin (3'-deoxyadenosine) on the production of inflammatory cytokines. Co-administration of 3.0 microg/ml cordycepin with LPS in cultured murine spleen cells significantly diminished the expression of the inflammatory cytokines tumor necrosis factor-alpha and interleukin-6 (IL-6) in a time-dependent manner. Expression of the inflammatory cytokine IL-17A was substantially down-regulated in a time- dependent manner at all cordycepin concentrations. These findings suggest that cordycepin down-regulates the immediate hypersensitivity reaction stimulated by LPS.
Subject(s)
Deoxyadenosines/metabolism , Immunosuppressive Agents/metabolism , Interleukin-17/biosynthesis , Interleukin-6/biosynthesis , Tumor Necrosis Factor-alpha/biosynthesis , Animals , Cells, Cultured , Leukocytes, Mononuclear/drug effects , Leukocytes, Mononuclear/immunology , Lipopolysaccharides/immunology , Mice , Mice, Inbred C57BL , Spleen/immunologyABSTRACT
We conducted a time course experiment on mycelial cultures of Laetiporus sulphureus var. miniatus. The strain showed significant survival in an initial pH range of 2.0 to 7.0 for 24 days, during which time oxalic acid was accumulated. A structural analysis of purified exopolysaccharide suggested that it contained 96.1% glucose, and the mode of linkage was mainly â 4-Glcp-(1 â units, with branches at the C-6 position consisting of a Glcp-(1 â 4) linked side chain. An exopolysaccharide purified from the acidophilic strain was added to cultured U937 cells, resulting in significantly increased transcription levels of p53 and p21 genes.
