ABSTRACT
Clinical and cytological studies in 41 hematological patients aged 19-70 years showed appreciable shifts in the immune status and nonspecific reactivity virtually in all examinees. Layers of cellular epithelium of late (V-VI) and early (III-IV) stages were more often detected in smears impressions than individual cells. Cells of stages I-II were scarce and never formed layers. In patients with leukemia, signs of dissociation of epitheliocyte nucleus and cytoplasm maturation were observed ("mature" cytoplasm and "young" nuclei). These specific shifts should be borne in mind when rendering dental care to hematological patients.
Subject(s)
Dental Care for Chronically Ill/methods , Hematologic Diseases/therapy , Mouth Diseases/therapy , Tooth Diseases/therapy , Acute Disease , Adult , Aged , Chronic Disease , Dental Care for Chronically Ill/statistics & numerical data , Female , Hematologic Diseases/complications , Hematologic Diseases/diagnosis , Humans , Male , Middle Aged , Mouth Diseases/diagnosis , Mouth Diseases/etiology , Oral Health , Tooth Diseases/diagnosis , Tooth Diseases/etiologyABSTRACT
Human intrathymic precursors are characterized by mitogenic reaction on thymic hormone, lacking the cortical and medullar thymic membrane markers (CD3, CD4 and/or CD8) and expressing of the common T-cell CD7 and CD5 antigens. Fraction 1.062 g/cm3 of peripheral white blood cells contains cells with analogous characteristics. The cell fraction of blood from men participated in amelioration after the Chernobyl accident is enriched with hemopoietic precursors (CD4+), pre-T-cells, reacting to thymosin (CD5+CD3-), and CD3+CD4-CD8- T-cells in a greater extent than the cell fraction from donors blood. Defects of T-cell and hemopoietic differentiation in children migrated from the Chernobyl area to Moscow Region were more substantial than in "ameliorators" and correlated with drop of thymic serum activity level. Research substantiates the approach to analysis of radiation-induced defects in early stages of lympho- and hemopoiesis.
Subject(s)
Hematopoietic Stem Cells/immunology , Power Plants , Radiation Injuries/immunology , Radioactive Hazard Release , T-Lymphocytes/immunology , Adolescent , Adult , Antibodies, Monoclonal , CD4-CD8 Ratio/radiation effects , Child , Hematopoietic Stem Cells/radiation effects , Humans , Immunophenotyping , Middle Aged , T-Lymphocytes/radiation effects , Time Factors , UkraineABSTRACT
Migration of FITC-labeled mouse bone marrow cells into the thymus was measured by flow cytometric analysis 3 hours after intravenous injection of cells into irradiated mice. The percentage of cells reaching the thymus diminished when the dose of injected cells increased. The dependence of the number or labeled cells in the thymus on the dose of injected cells was not linear. Pretreatment of cells with anti-SC-I serum, peanut lectin or H-2 incompatibility antigen abolished thymus migration, while treatment with anti-Thy-I serum, soybean lectin, trypsin or Thy-I-incompatibility antigen diminished cellular migration and treatment with neuraminidase enhanced it. It was concluded that the main type of migrating cells is SC-1+ precursors of T-lymphocytes. Penetration of these cells through the blood-thymus barrier is based on the recognition of their partly sialized surface glycoprotein receptors by membrane lectins of the blood-thymus barrier cells.
