Subject(s)
Ovum/cytology , Pollen/cytology , Sputum/cytology , Toxocara/isolation & purification , Animals , Humans , Male , Middle Aged , Sputum/parasitologySubject(s)
Feces/microbiology , Feces/parasitology , Inhalation Exposure , Lung Diseases/etiology , Mites/microbiology , Mites/parasitology , Animals , HumansSubject(s)
Daphnia , Sputum/parasitology , Animals , Humans , Incidental Findings , Specimen Handling , Sputum/cytology , Vaginal Smears/methodsABSTRACT
BACKGROUND: Pulmonary aspiration of vegetable matter may be a serious cause of lung damage, requiring, almost always, the help of a histopathologic examination. CASE: We report the case of a 55-year-old woman with a granulomatous pneumonitis due to repeated pulmonary aspiration of vegetable particles and affected by a syringomyelia. Histologic findings from a lobectomy section are shown and compared with those observed in different cytologic sputum samples. CONCLUSION: The recognition of uncommon morphologic structures, and their comparison with others already known, both in histologic sections and in cellular smears, may be useful when it comes to establishing a sure diagnosis.
Subject(s)
Foreign Bodies/pathology , Pneumonia, Aspiration/pathology , Sputum/cytology , Syringomyelia/pathology , Vegetables , Cytodiagnosis/methods , Female , Foreign Bodies/complications , Foreign Bodies/surgery , Granuloma/pathology , Granuloma/surgery , Humans , Lung/pathology , Lung/surgery , Middle Aged , Pneumonia, Aspiration/complications , Pneumonia, Aspiration/surgery , Syringomyelia/complicationsSubject(s)
Enterobius/cytology , Ovum/cytology , Papanicolaou Test , Vaginal Smears , Animals , Female , Humans , Middle AgedABSTRACT
The aim of this study was to pilot a simpler and more effective method for identifying dust mites in sputum. Dust mites and their allergens have been implicated in respiratory diseases, including pulmonary acariasis, and several studies have identified mites in sputum. Further research is dependent on the development of a faster and simpler diagnostic test. We have demonstrated that dust mites artificially introduced into sputa, could be identified after the sputa were liquefied with bleach, when the liquid sample was observed under the microscope. Liquefaction times for serous, mucous, purulent and hemoptoic sputa varied from 10 to 30 min (mean 17.5). The test had a sensitivity of 80% (95% CI 68.2-88.2%) as 46/60 mites were identified. This procedure can be performed quickly at room temperature, is simple, inexpensive, repeatable, and less labourious than previous methods.
Subject(s)
Lung Diseases/diagnosis , Mite Infestations/diagnosis , Pyroglyphidae , Sputum/parasitology , Animals , Sodium HypochloriteSubject(s)
Erythrocytes/pathology , Fungi/cytology , Lung Diseases, Fungal/microbiology , Lung Diseases, Fungal/pathology , Lung/microbiology , Lung/pathology , Artifacts , Biopsy, Fine-Needle , Cryptococcus neoformans/cytology , Diagnosis, Differential , Diagnostic Errors/prevention & control , Humans , Lung/physiopathology , Lung Diseases, Fungal/physiopathology , Pneumocystis carinii/cytology , Sputum/cytologyABSTRACT
The presence of worms in cytological smears is occasionally reported, although various other structures exist that may be confused with such parasites. We present eight worm-like artifacts observed in routine Papanicolaou smears. Recognition of these structures is important to avoid overvaluation or confusion with true worms.
Subject(s)
Artifacts , Diagnostic Errors/prevention & control , Helminthiasis/pathology , Helminths/cytology , Vaginal Smears , Animals , HumansSubject(s)
Amoeba/isolation & purification , Equipment Contamination , Sputum/parasitology , Water/parasitology , Animals , Humans , Water SupplySubject(s)
Diagnostic Errors/prevention & control , Helminthiasis/diagnosis , Helminths/embryology , Ovum/cytology , Sputum/cytology , Animals , Artifacts , Diatoms/cytology , Equipment Contamination/prevention & control , Fungi/cytology , Helminths/cytology , Humans , Pollen/cytology , Spores, Fungal/cytology , Vaginal Smears/methodsABSTRACT
Several types of contaminants have been reported in cytopathology. The purpose of this study was to show unusual contaminants in cytological smears, such as freshwater microorganisms and other arthropods. In different routine Papanicolaou smears (sputum, cervicovaginal, and voided urine) we mainly found fragments of mites and insects, caused by an intrinsic contamination of the samples or an extrinsic contamination of the smears.
Subject(s)
Arthropods , Papanicolaou Test , Sputum , Vaginal Smears , Animals , Female , HumansABSTRACT
The objective was to demonstrate the presence of airborne fungal spores, pollen grains, and vegetable cells in smears and establish their significance. Microscopic examination was of smears stained by the Papanicolaou technique. We found several types of airborne spores (Alternaria, Exserohilum, Aspergillus, Cladosporium, Epicoccum, Curvularia, and Ascosmycete), pollen grains (Lilium, bullrush, Pinus, Acacia, hazelnut, and oak), and several types of vegetable cells. The existence of these structures may be due to intrinsic or to extrinsic contamination of the sample. Anemophilous fungi spores and pollen grains have been shown to possess great allergenic capacity. Pollen grains and vegetable cells may be mistaken for certain microorganisms or malignant cells.
Subject(s)
Edible Grain/cytology , Pollen/cytology , Specimen Handling , Spores, Fungal/cytology , Vegetables/cytology , Cytodiagnosis , Diagnostic Errors , HumansABSTRACT
Planteamiento: Describir una serie de contaminantes a través de la observación microscópica de extendidos citológicos de esputos. Material y métodos: Extendidos de esputos coloreados mediante la técnica de Papanicolaou. Resultados: Hemos constatado, en 15.000 muestras de pacientes ingresados con diferentes patologías respiratorias durante los últimos 10 años, la presencia de diferentes tipos de microalgas, células vegetales, granos de polen y diversos cristales. Conclusiones: La existencia de contaminantes, en extendidos de esputos, es un hecho relativamente frecuente, teniendo un cierto significado biológico y debiéndose establecer en ocasiones su diagnóstico diferencial, dadas las similitudes con otras estructuras tales como hongos y diversos protozoos (AU)
